Abstract:

Objective To assess the protective effect of adipose⁃derived mesenchymal stem cells（ADSCs） combined with dexmedetomidine on acute ischemia⁃reperfusion injury（IRI）in rats. Methods Fortyadult healthy SD male rats were randomly divided into sham⁃operated group，ischemia reperfusion injury group（IR group）， dexmedetomidine⁃treated group（IR+DEX group），ADSCs⁃treated group（IR+ADSCs group）and dexmedetomidine combined with ADSCs⁃treated group（IR+DEX+ADSCs group）. Isolated ADSCs were cultured in suitable incuba⁃ tion conditions and the IRI model in rats was established. The rats received cell suspension of ADSCs（1 × 106） through tail vein or/and received intraperitoneal injection of dexmedetomidine 25 μ g/kg in different groups. The plasma samples of each group were harvested for determining the level of serum creatinine（Scr）and blood urea nitrogen（BUN）at 0，24 h and 96 h. Urine samples of rats of each group were collected at the same time for detecting urine protein（UP）and urine creatinine（UC），and calculating the ratio of urine protein/ creatinine （UP/UC）. The rats were euthanized after anesthetization and kidney specimens were collected for HE staining at 96 h after the experimental treatment，and the histopathology injury of kidney were scored. Results When compared with that in IR group，the level of Scr，BUN and UP/UC decreased significantly in IR+DEX group and IR+ADSCs group（P < 0.05），meanwhile the injury of kidney was also significantly reduced in two groups. When compared with that in IR+DEX group and IR+ADSCs group，the above indicators in IR+DEX+ADSCs group further decreased and was statistically significant（P < 0.05）. Conclusion In rat model of acute renal ischemia⁃reperfusion injury， the combination of ADSCs with dexmedetomidine has better renal protection effect when compared with single ADSCs or dexmedetomidine treatment. 

Key words:

kidney ischechemia?reperfusion, dexmedetomidine, adiposed?derived mesenchymal stem cells, renal function