Abstract:

Objective To elucidate the role of mutual regulation of IRE1a and miR ⁃346 in maintaining ERS strength，and it′s role in cisplatin resistance of cervical cancer cells. Methods We screened cisplatin⁃resistant cervical cancer cells via gradually increasing the concentration of cisplatin，and detected the expression of IRE1a and miR⁃346 via gene array assay，quality PCR assay，flow cytometry assay and dual⁃luciferase reporter assay. We further validated the mutual regulation between IRE1a and miR⁃346，and its role in cispaltin resistance of cervical cancer cells. Results In contrast with the parental cervical cancer cells，there was a basic ERS in cisplatin resistant cells，and ERS enhanced the expression of miR⁃346 in IRE1a dependent manner which took part in the cisplatin resistant of cervical cancer cells. MiR⁃346 down⁃regulated the expression of IRE1a，which inhibited the degrada⁃ tion of pri⁃miR⁃125b，maintained the ERS strength in cisplatin⁃resistant cell and promoted cellular survival under cispaltin treatment. Conclusion Mutual regulation of IRE1a and miR⁃346 promotes cispaltin resistance of cervical cancer cells through maintaining ERS strength of cisplatin⁃resistant cells.

Key words:

cervical cancer, edoplasmic reticulum stress, IRE1a, miR?346, cisplatin resistance