抗阻运动调节Piezo1对老年小鼠骨骼肌成肌细胞的影响

doi:10.3969/j.issn.1006-5725.2025.23.002

Abstract

Abstract:

Objective To investigate the regulatory effects of resistance exercise on Piezo1 in myoblasts within skeletal muscles of aged mice. Methods In animal experiments， thirty 20-month-old male C57BL/6 mice were randomly assigned to one of three groups： an aging control group， an aging exercise group， and an aging exercise inhibitor group. Additionally， ten 3-month-old young mice served as a young control group. The aging exercise group underwent 8 weeks of resistance exercise training， whereas the aging exercise inhibitor group received both 8 weeks of resistance exercise and intraperitoneal injections of GsMTx4. Exercise capacity was evaluated using grip strength tests and rotarod performance assays. Skeletal muscle cross-sectional area was assessed via hematoxylin-eosin staining， fibrosis was examined using Sirius red staining， and the proportion of p16-positive cells was determined by immunohistochemistry. In cellular experiments， senescence in myoblasts was induced with D-galactose （D-gal）， followed by activation of Piezo1 using Yoda1. The percentage of senescent cells was measured by SA-β-Gal staining， while protein expression levels of senescence markers （p16 and p21） and atrophy markers （Atrogin-1 and MuRF1） were quantified by Western blot analysis. Cell immunofluorescence staining was employed to assess the average fluorescence intensity of p21. Results （1）Compared with the aging control group， the aging + exercise group exhibited significant improvements in exercise capacity， quadriceps femoris mass index， skeletal muscle cross-sectional area， reduction in skeletal muscle fibrosis， and a lower p16-positive cell percentage （P < 0.05）；（2）These beneficial effects of exercise on aged skeletal muscle were attenuated following treatment with a Piezo1 inhibitor （P < 0.05）；（3）In vitro experiments demonstrated that activation of Piezo1 reduced the SA-β-Gal positive cell rate in myoblasts， downregulated the expression of senescence markers （p16 and p21） and atrophy-related markers （Atrogin-1 and MuRF1）， and decreased the average fluorescence intensity of p21 （P < 0.05）. Conclusion Resistance exercise suppresses myocyte aging through activation of Piezo1， thereby ameliorating age-related muscle atrophy in the elderly.

Key words: annual muscular atrophy, resistance exercise, Piezo1, aging, skeletal muscles

CLC Number:

R459.9

Rong LIN,Gang. LIU. The effect of resistance exercise regulation of Piezo1 on myoblasts in skeletal muscles in elderly mice[J]. The Journal of Practical Medicine, 2025, 41(23): 3638-3644.

Figures/Tables 12

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References 34

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组别	抓力/N	转棒力竭时间/s
青年对照组	1.802 ± 0.089	248.100 ± 46.900
衰老对照组	0.982 ± 0.145^△△△	107.400 ± 66.730^△△△
衰老+运动组	1.570 ± 0.218^▲▲	204.200 ± 73.690^▲▲▲
衰老+运动+抑制剂组	0.988 ± 0.115^○○○	88.500 ± 18.230^○○○
F值	77.28	18.88
P值	< 0.001	< 0.001

组别	SA-β-Gal阳性/%	p21平均荧光强度/a.u.
空白对照组	0.051 ± 0.026	0.242 ± 0.086
D-gal组	0.319 ± 0.116^△△△	0.547 ± 0.115^△△△
D-gal + Yoda1组	0.104 ± 0.041^▲▲▲	0.190 ± 0.074^▲▲▲
F值	34.36	38.44
P值	< 0.001	< 0.001

组别	p16	p21
空白对照组	0.472 ± 0.121	0.242 ± 0.086
D-gal组	1.316 ± 0.139^△△△	0.547 ± 0.115^△△△
D-gal + Yoda1组	0.396 ± 0.130^▲▲▲	0.190 ± 0.074^▲▲▲
F值	138.7	38.44
P值	< 0.001	< 0.001

组别	Atrogin-1	MuRF-1
空白对照组	0.630 ± 0.122	0.566 ± 0.111
D-gal组	1.293 ± 0.133^△△△	1.293 ± 0.187^△△△
D-gal + Yoda1组	0.828 ± 0.108^▲▲▲	0.733 ± 0.123^▲▲▲
F值	70.72	63.04
P值	< 0.001	< 0.001

The effect of resistance exercise regulation of Piezo1 on myoblasts in skeletal muscles in elderly mice

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