miR-223通过调控Keap1/Nrf2/ARE信号通路对前列腺癌细胞损伤的影响

doi:10.3969/j.issn.1006-5725.2024.17.004

Abstract

Abstract:

Objective To investigate the effect of microRNA-223 （miR-223） on prostate cancer cell damage by regulating the Kelch?like epichlorohydrin related protein 1 （Keap1）/nuclear factor E2 related factor 2 （Nrf2）/antioxidant response element （ARE） signaling pathway. Methods The prostate cancer cell line PC3 was cultured and randomly divided into control， down-regulated miR-223， and up-regulated miR-223 groups. Changes in miR-223 expression， cell proliferation rate， cell migration number， cell invasion number， apoptosis rate， and expression level of Keap1/Nrf2/ARE signaling pathway were explored. Results Compared with the control group， the cell invasion number， cell migration number， cell proliferation rate， Nrf2 and ARE expression increased at 24， 48 and 72 h in down-regulated miR-223 group， while the expressions of miR-223， Keap1 and apoptosis rate decreased （P < 0.05）. Compared with the down-regulated miR-223 group， 24， 48 and 72 h cell proliferation rate， cell invasion number， cell migration number， ARE and Nrf2 expression decreased in the up-regulated miR-223 group， while miR-223， apoptosis rate and Keap1 expression increased （P < 0.05）. Conclusion Regulation of miR-223 effectively ameliorates prostate cell injury， and the mechanism may be related to the Keap1/Nrf2/ARE signaling pathway.

Key words: prostate cancer, microRNA-223, Kelch-like epichlorohydrin associated protein 1/nuclear factor E2 related factor 2/antioxidant response element, cell injury

CLC Number:

R361⁺.3

Zhishi WANG,Guiling LI,Jingguo CHEN,Hong. WANG. Effects of miR⁃223 on prostate cancer cell damage by regulating Keap1/Nrf2/ARE signaling pathway[J]. The Journal of Practical Medicine, 2024, 40(17): 2375-2380.

Figures/Tables 8

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References 26

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组别	miR-223
对照组	0.76 ± 0.10^a
下调miR-223组	0.45 ± 0.06
上调miR-223组	0.62 ± 0.07^ab
F值	9.762
P值	0.001

组别	细胞增殖率			凋亡率
组别	24 h	48 h	72 h	凋亡率
对照组	1.25 ± 0.22^a	1.86 ± 0.25^a	2.17 ± 0.30^a	2.54 ± 0.23^a
下调miR-223组	3.45 ± 0.43	3.82 ± 0.50	3.95 ± 0.62	1.72 ± 0.18
上调miR-223组	2.02 ± 0.31^ab	2.19 ± 0.34^ab	2.41 ± 0.41^ab	5.21 ± 0.50^ab
F值	14.400	11.090	8.120	8.878
P值	0.001	0.001	0.001	0.001

组别	细胞侵袭数	细胞迁移数
对照组	60.46 ± 5.23^a	37.02 ± 5.19^a
下调miR-223组	79.17 ± 8.50	55.46 ± 7.10
上调miR-223组	65.22 ± 6.64^ab	43.53 ± 6.21^ab
F值	5.928	6.630
P值	0.001	0.001

组别	Keap1	Nrf2	ARE
对照组	1.00 ± 0.01^a	1.00 ± 0.01^a	1.00 ± 0.01^a
下调miR-223组	0.39 ± 0.10	1.02 ± 0.20	1.06 ± 0.21
上调miR-223组	0.84 ± 0.19^ab	0.63 ± 0.14^ab	0.60 ± 0.15^ab
F值	19.190	5.052	5.637
P值	0.001	0.001	0.001

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Effects of miR⁃223 on prostate cancer cell damage by regulating Keap1/Nrf2/ARE signaling pathway

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