年轻干细胞抗原-1阳性骨髓干细胞调控年老小鼠心脏成纤维细胞凋亡的分子机制

doi:10.3969/j.issn.1006-5725.2024.17.003

Abstract

Abstract:

Objective To investigate the impact of Sca-1 bone marrow derived stem cells on apoptosis in murine cardiac fibroblasts and the molecular mechanisms of young （Y） Sca?1 bone marrow stem cell （BMSC） on old （O） cardiac fibroblast cell （CFC） apoptosis. Methods The apoptosis and survival of Y and O CFC were assessed under hypoxic conditions. Co-cultures of Y and O Sca-1 bone marrow-derived mesenchymal stem cells （BMSC） with O CFC were established to investigate the impact of Sca-1 BMSC on the apoptotic response and viability of O CFC， employing TUNEL staining， qRT-PCR， Western Blot， and CCK8 assays. Furthermore， differential secretion profiles of growth factors by Y and O Sca-1 BMSC were compared using qRT-PCR and ELISA analysis. Results Compared to Y CFC， O CFC exhibited an increased rate of apoptosis and a decreased rate of cell survival. However， when compared to O cells， Y Sca-1 BMC significantly reduced apoptosis in O CFC and enhanced cell survival. Moreover， Y Sca-1 BMSC demonstrated a higher secretion of GDF5 （Growth Differentiation Factor 5） than O cells （P < 0.05）. Importantly， the protective effects of Y Sca-1 BMSC on apoptosis and survival in O CFC were abolished upon neutralization of GDF5 expression. Conclusion Y Sca-1 BMSC decreases O CFC apoptosis through GDF5.

Key words: stem cell, cardiac fibroblast cell, apoptosis, cell survival, growth factors, Sca-1

CLC Number:

R363.1

Rao LÜ,Jiadi YU,Liuzhen LI,Chulan ZHAN,Liyue ZHAO,Yueliang LI,Jun DONG,Jiao. LI. Molecular mechanism of young Sca⁃1 bone marrow stem cell on old cardiac fibroblast cell apoptosis in aging mice[J]. The Journal of Practical Medicine, 2024, 40(17): 2369-2374.

Figures/Tables 5

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References 25

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引物名称	上游引物	下游引物
GAPDH	AACGGATTTGGTCGTATTG	GGAAGATGGTGATGGGATT
Mcl1	TTGTAAGGACGAAACGGGAC	TCTAGGTCCTGTACGTGGAAG
Puma	CTGGAGGGTCATGTACAATCTC	GGTGTCAGAAGGCGGAG
Gdf5	GACAGAAAGGGAGGTAACAGC	TTCTCCAAGGCACTGATGTC

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Molecular mechanism of young Sca⁃1 bone marrow stem cell on old cardiac fibroblast cell apoptosis in aging mice

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