基于NGF/TrKA/TRPV1通路探讨电针改善功能性消化不良大鼠胃高敏感性

doi:10.3969/j.issn.1006-5725.2023.22.012

Abstract

Abstract:

Objective To explore the effect and mechanism of electroacupuncture （EA） on gastric hypersensitivity in rats with functional dyspepsia （FD）. Methods Rats were randomly divided into blank group， model group， EA group， inhibitor group and EA+inhibitor group. The blank group was not subjected to special treatment， and the model group， EA group， inhibitor group， and EA+inhibitor group were modeled using a multi factor stimulation method. After the modeling， the model group was given routine feeding， the EA group and the EA+inhibitor group were treated with daily electroacupuncture at Zusanli， and the inhibitor group and the EA+ inhibitor group were treated with daily intraperitoneal injection of anti-nerve growth factor（NGF）. Gastric hypersensitivity and gastric compliance were detected by intragastric balloon implantation， the degree of mast cell activation were observed by methylamine blue staining， the localization expression of gastric transient receptor potential vanillin subtype 1（TRPV1） was detected by immunohistochemistry， the expression of gastric NGF/tropomyosin receptor kinase （TrkA）/TRPV1 protein was detected by Western blot， and the content of gastric calcitonin gene-related peptide （CGRP） was detected by enzyme-linked immunosorbent assay. Results Compared with the blank group， the weight of the model group rats increased slowly， gastric sensitivity increased， compliance decreased， the number of gastric mast cell and degranulation rate increased significantly， and the protein expression and CGRP content of NGF， TrKA， TRPV1 increased significantly. Compared with the model group， the rats in the EA group， the inhibitor group and the EA+inhibitor group gained weight， improved gastric hypersensitivity and compliance significantly， reduced the number and activation of mast cell significantly， and reduced gastric NGF/TrKA/TRPV1 protein expression and CGRP content significantly. Compared with the EA group and the inhibitor group， the expression of NGF/TrKA/TRPV1 protein and CGRP content in the stomach of the EA+inhibitor group were further reduced， and the difference was statistically significant. Conclusion EA may relieve the gastric hypersensitivity of FD rats by regulating the NGF/TrKA/TRPV1 pathway activated by mast cells.

Key words: functional dyspepsia, gastric hypersensitivity, mast cells, nerve growth factor/tropomyosin receptor kinase, transient receptor potential vanilloid type 1

CLC Number:

R245.3

Shuwen JIN,Wei LIU,Jiabao LIU,Jianchao FAN,Yongli HAN,Li ZHOU,Paidi XU,Hongxing. ZHANG. Mechanism of electroacupuncture for gastric hypersensitivity in functional dyspepsia rats rats through NGF/TrKA/TRPV1 pathway[J]. The Journal of Practical Medicine, 2023, 39(22): 2928-2933.

Figures/Tables 11

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References 23

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组别	例数	基线期	造模后	干预后
空白组	6	16.36 ± 0.69	263.65 ± 7.50	381.12 ± 21.00
模型组	6	17.20 ± 0.91	179.07 ± 13.62^*	260.20 ± 8.97^*
电针组	6	16.83 ± 0.74	169.05 ± 11.00^*	304.00 ± 13.14^*#
抑制剂组	6	17.17 ± 0.52	172.58 ± 12.20^*	291.58 ± 12.21^*#
电针+抑制剂组	6	16.65 ± 0.68	171.28 ± 9.82^*	306.38 ± 14.33^*#
F值		1.20	68.27	43.11
P值		0.334	< 0.001	< 0.001

组别	例数	20 mmHg	40 mmHg	60 mmHg	80 mmHg
空白组	6	0.16 ± 0.01	0.20 ± 0.02	0.25 ± 0.02	0.27 ± 0.01
模型组	6	0.14 ± 0.02	0.17 ± 0.01^*	0.19 ± 0.01^*	0.18 ± 0.01^*
电针组	6	0.15 ± 0.01	0.19 ± 0.01^#	0.23 ± 0.02^#	0.22 ± 0.01^#
抑制剂组	6	0.15 ± 0.02	0.19 ± 0.01^#	0.22 ± 0.01^#	0.21 ± 0.02^#
电针+抑制剂组	6	0.15 ± 0.01	0.19 ± 0.01^#	0.23 ± 0.01^#	0.23 ± 0.01^#
F值		1.294	6.209	14.08	32.84
P值		0.2993	< 0.01	< 0.001	< 0.001

组别	例数	20 mmHg	40 mmHg	60 mmHg	80 mmHg
空白组	6	0.33 ± 0.47	1.33 ± 0.47	2.00 ± 0.82	2.67 ± 0.47
模型组	6	0.67 ± 0.47	2.83 ± 0.69^*	3.50 ± 0.50^*	3.83 ± 0.37^*
电针组	6	0.5 ± 0.5	2.33 ± 0.47	2.50 ± 0.50^#	3.16 ± 0.37^#
抑制剂组	6	0.67 ± 0.47	2.67 ± 0.75	2.67 ± 0.47^#	3.16 ± 0.37^#
电针+抑制剂组	6	0.83 ± 0.37	2.17 ± 0.69	2.33 ± 0.47^#	3.00 ± 0.58^#
F值		0.86	4.383	12.92	4.643
P值		0.50	< 0.01	< 0.001	< 0.01

组别	例数	肥大细胞记数（个）	肥大细胞脱颗粒率（%）
空白组	6	4.83 ± 2.67	16.67 ± 13.05
模型组	6	16.50 ± 3.99^*	67.64 ± 15.11^*
电针组	6	9.00 ± 4.00^#	31.84 ± 16.21^#
抑制剂组	6	10.50 ± 4.37^#	38.73 ± 11.00^#
电针+抑制剂组	6	7.83 ± 4.37^#	25.97 ± 14.50^#
F值		6.094	9.449
P值		< 0.01	< 0.001

组别	例数	TRPV1平均光密度值
空白组	6	0.013 ± 0.001
模型组	6	0.063 ± 0.008^*
电针组	6	0.037 ± 0.005^#△
抑制剂组	6	0.039 ± 0.005^#△
电针+抑制剂组	6	0.025 ± 0.004^#
F值		64.71
P值		< 0.001

Mechanism of electroacupuncture for gastric hypersensitivity in functional dyspepsia rats rats through NGF/TrKA/TRPV1 pathway

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组别	例数	NGF	TrKA	TRPV1
空白组	6	0.62 ± 0.05	0.40 ± 0.03	0.36 ± 0.03
模型组	6	0.91 ± 0.02^*	1.19 ± 0.03^*	1.20 ± 0.03^*
电针组	6	0.74 ± 0.05^#△	0.57 ± 0.03^#△	0.57 ± 0.03^#△
抑制剂组	6	0.76 ± 0.04^#△	0.60 ± 0.02^#△	0.59 ± 0.02^#△
电针+抑制剂组	6	0.66 ± 0.04^#	0.49 ± 0.02^#	0.49 ± 0.02^#
F值		38.3	742.9	826.0
P值		< 0.001	< 0.001	< 0.001

组别	例数	CGRP含量（pg/mg）
空白组	6	1.30 ± 0.04
模型组	6	2.04 ± 0.16^*
电针组	6	1.73 ± 0.09^#△
抑制剂组	6	1.79 ± 0.10^#△
电针+抑制剂组	6	1.57 ± 0.08^#
F值		36.32
P值		< 0.001

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