Abstract:

Objective To observe the regulatory effect of emodin（ED）on the expressions of procoagulant and fibrinolysis inhibitory factors in alveolar epithelial cells type Ⅱ（AECⅡ）under LPS stimulation. Methods Cells were given different doses of ED（0.25，0.5，1 μg/mL）treated for 1h and then stimulated with LPS for 24 h. The expressions of TF，TFPI，PAI⁃1 in the cells and the levels of PⅢP，TAT，AT Ⅲ and APC in the cell superna⁃ tant were detected. Results The expressions of TF and PAI⁃1 in LPS stimulated cells significantly increased，while the expression of TFPI decreased. At the same time ，the levels of PⅢP and TAT in the supernatant of cells increased，while the levels of AT Ⅲ and APC decreased，which all were reversed by ED in a dose ⁃dependent manner. Immunofluorescence suggested that LPS promoted the expression of p65 in nucleus，which was significantly inhibited by ED. Conclusion ED，in the dose⁃dependent method，inhibits the expression and secretion of proco⁃ agulant and fibrinolytic inhibition factors in AECⅡ cells stimulated by LPS，which may be related to its inhibition of NF⁃κB signal pathway.

Key words:

emodin, alveolar epithelial cell type Ⅱ, acute respiratory distress syndrome, coagula? tion, fibrinolysis