Abstract:

Objective To explore the role of glycoprotein Serglycin in chemoresistance and metastasis of epithelial ovarian cancer. Methods The stable interference cell line was established from epithelial ovarian cancer SKOV3 cell line by interfering with Serglycin expression using shRNA technology，and Western blot technology and qRT ⁃PCR were employed to verify knockdown efficiency. MTS method was used to detect the sensitivity of ovarian cancer cell line with stable knockdown of Serglycin to the chemotherapeutic drug cisplatin. Transwell cell was used to detect the invasion and metastatic ability of ovarian cancer cell lines with stable knockdown of Serglycin；Western blot was used to detect the effect of Serglycin on EMT ⁃ related molecules. In addition，online prognosis analysis software（kmplot）was used to analyze the correlation between Serglycin and the prognosis of patients with epithelial ovarian cancer. Results Knockdown of Serglycin cells significantly enhanced the sensitivity of epithelial ovarian cancer cells to the chemotherapy drug cisplatin and significantly inhibited the invasion and metastasis ability of ep⁃ ithelial ovarian cancer cells compared with the control group. The knockdown promoted the EMT changes of epithelial ovarian cancer cells via ZEB2. The overall survival of the patients with low expression of Serglycin was significantly longer than those with high expression（HR = 1.26，P＜0.001）. Conclusion Serglycin promotes EMT changes of ovarian cancer cells via ZEB2，and then promotes chemoresistance and invasion and metastasis of ovarian cancer cells.

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