他喹莫德对人胶质母细胞瘤细胞恶性表型的影响及相关机制

doi:10.3969/j.issn.1006-5725.2023.16.004

Abstract

Abstract:

Objective To investigate the effects and related mechanisms of Tasquinimod （Tasq） on malignant phenotypes such as proliferation， migration， epithelial-mesenchymal transition （EMT）， stem cell characteristics in glioblastoma multiforme（GBM）cells， so as to provide reference for preliminary experiment of Tasq as a potential drug treating GBM. Methods The effects of Tasq on the proliferation， apoptosis， migration and colony formation of GBM cell lines （U87 and U251） were evaluated by CCK?8， apoptosis staining， transwell assay and colony formation assay. The expression of apoptosis?related proteins and EMT?related proteins were detected by western blot. The effect of Tasq on spheres formation of glioma stem cell （GSC） was detected by sphere formation assay， and the expression of stem cell?related proteins in GSC were detected by immunofluorescence staining and Western blot. Results Tasq inhibited the proliferation of U87 and U251 in concentration?dependent and time?dependent manner； Tasq also inhibited the migration and colony formation of U87 and U251， and promoted its apoptosis；Tasq promoted the expression of apoptotic protein Cleaved Caspase?3 and decreased the expression of anti?apoptotic protein BCL?2， EMT?related proteins （Snail，Vimentin）. Tasq also reduced the spheroid formation ability of GSC and the expression of stem cell?related proteins （SOX2，Nestin）， the differences were statistically significant while compared with that in the control group（P < 0.01）. Conclusion Tasq inhibits proliferation， migration， colony formation of GBM cells and promoted its apoptosis in vitro. Tasq may inhibit the malignant progression of GBM cells by inhibiting EMT and stem cell characteristics.

Key words: Tasquinimod, glioblastoma cell, EMT, stem cell characteristics

CLC Number:

R739.41

Xiaorui LIU,Zhongmin YUAN,Cheng ZHI,Meimei ZHANG,Xiaoshun JIAN,Huaidong. PENG. Preliminary study on the effects and related mechanisms of Taquimod on malignant phenotype of human glioblastoma cells[J]. The Journal of Practical Medicine, 2023, 39(16): 2029-2036.

Figures/Tables 7

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Preliminary study on the effects and related mechanisms of Taquimod on malignant phenotype of human glioblastoma cells

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