实用医学杂志 ›› 2021, Vol. 37 ›› Issue (3): 319-323.doi: 10.3969/j.issn.1006⁃5725.2021.03.009

• 临床研究 • 上一篇    下一篇

IL⁃6/STAT3通路在肺结核 作用机制 患者巨噬细胞免疫中的

鲁进,尹伶,黄汉平
  

  1. 1 武汉科技大学医学院(武汉 430081);2 鄂州市第三医院感染科(湖北鄂州 436000);3 武汉市金银潭医院结核科(武汉 430023)
  • 出版日期:2021-02-10 发布日期:2021-02-10
  • 通讯作者: 尹伶 E⁃mail:xjjzr7871@sohu.com
  • 基金资助:
    国家重大专项项目(“十二五”科技重大专项科研基金)(编号:2015ZX10003001)

Mechanism of IL ⁃ 6/STAT3 pathway in macrophage immunity of patients with pulmonary tuberculosis

LU Jin,YIN Ling,HUANG Hanping
  

  1. Medical College,Wuhan University of Science & Technology,Wuhan,430081,China
  • Online:2021-02-10 Published:2021-02-10
  • Contact: YIN Ling E⁃mail:xjjzr7871@sohu.com

摘要:

目的 探讨白细胞介素⁃6(IL⁃6)/信号转导和转录激活因子 3(STAT3)通路在肺结核患者巨 噬细胞免疫中的作用机制。方法 选取 2017 10 月至 2019 10 月在武汉市金银潭医院接受治疗的肺 结核患者 92 例(观察组)作为研究对象,同期健康体检者 95 例(对照组)作为对照,收集受试者空腹外周 静脉血,蛋白免疫印迹(Western blot)法检测外周血中 STAT3、p⁃STAT3、核因子κB(NF⁃κB)p65、p⁃NF⁃κB p65 蛋白表达水平,酶联免疫(ELISA)法检测血清炎症因子 IL⁃6、IL⁃10、肿瘤坏死因子⁃α(TNF⁃α)水平。体 外培养巨噬细胞,分为正常组(不做处理)和感染组(感染结核分枝杆菌),Western blot 法检测巨噬细胞中 STAT3、p⁃STAT3、NF⁃κB p65、p⁃NF⁃κB p65 蛋白表达水平,ELISA 法检测巨噬细胞上清液中炎症因子 IL⁃6 IL⁃10、TNF⁃α水平。结果 与对照组相比,观察组肺结核患者外周血中p⁃STAT3/STAT3、p⁃NF⁃κB p65/NF⁃κB p65 蛋白水平,血清中 IL⁃6、IL⁃10、TNF⁃α表达水平显著升高(P<0.05);与正常组相比,感染组巨噬细胞中 p⁃STAT3/STAT3、p⁃NF⁃κB p65/NF⁃κB p65 蛋白表达水平,巨噬细胞上清液中 IL⁃6、IL⁃10、TNF⁃α表达水平显 著升高(P<0.05)。结论 IL⁃6/STAT3 通路在肺结核患者及感染结核分枝杆菌的巨噬细胞中处于激活状 态,巨噬细胞可能通过激活IL⁃6/STAT3

关键词:

Abstract:

Objective To investigate the mechanism of interleukin(IL)⁃6/signal transducer and activator of transcription 3(STAT3)pathway in macrophage immunity of patients with pulmonary tuberculosis. Methods A total of 92 cases of pulmonary tuberculosis patients(observation group)treated in Wuhan Jinyintan Hospital from October 2017 to October 2019 were recruited as the research objects,and 95 healthy persons(control group were selected as the control group. Fasting peripheral venous blood was collected. The protein expression levels of STAT3,p⁃STAT3,Nuclear factor⁃κB(NF⁃κB)p65 and p⁃NF⁃κB p65 were detected by Western blot. The levels of serum inflammatory factors IL⁃6,IL⁃10 and tumor necrosis factor⁃α(TNF⁃α)were detected by enzyme⁃linked immunosorbent assay(ELISA). Macrophages were cultured in vitro and divided into normal group(without treat⁃ ment)and infection group(infected with Mycobacterium tuberculosis). Western blot was used to detect the protein expression levels of STAT3,p⁃STAT3,NF⁃κB p65 and p⁃NF⁃κB p65 in macrophages,and the levels of IL⁃6,IL⁃10 and TNF⁃α in macrophage supernatant were detected by ELISA. Results Compared with those in the control group the protein levels of p⁃STAT3/STAT3,p⁃NF⁃κB p65/NF⁃κB p65 in the peripheral blood,and the expression levels of IL⁃6,IL⁃10 and TNF⁃α in serum in the observation group were significantly higher than those in the control group (P < 0.05). Compared with those in the normal group,the protein levels of p⁃STAT3/STAT3,p⁃NF⁃κB p65/NF⁃κB p65 in macrophages,and the expression levels of IL⁃6,IL⁃10 and TNF⁃α in supernatant of macrophage in infected group were significantly higher than those in normal group(P < 0.05). Conclusion IL⁃6/STAT3 pathway is activated in pulmonary tuberculosis patients and macrophages infected with Mycobacterium tuberculosis. Macrophages may play an immunomodulatory role in pulmonary tuberculosis by activating IL⁃6/STAT3 pathway.

Key words: