Abstract:

Objective To study the changes in body weight，blood lipids，blood sugar and LPS of miceon a high⁃fat diet under the intervention of calcium chloride，use 16S RNA high⁃throughput sequencing technologyto detect changes in the intestinal flora of high⁃fat mice，and explore the effect of calcium chloride on obesity in themice by regulation the composition of gut microbes. Methods Thirty male C57BL/6 wild⁃type mice were randomlydivided into control group（C），model group（M），and calcium chloride treatment group（D），10 in each group.Groups C，M and D were fed with standard feed plus drinking water，high⁃fat feed plus drinking water，and high⁃fat feed plus 1.5 g/100 mL calcium chloride for 11 weeks，followed by collecting the samples. The changes in bodyweight，blood lipids，blood sugar，LPS，and intestinal flora of different groups of mice were compared among thegroups. Results Calcium chloride significantly inhibited the weight gain of C57BL/6J mice on a high ⁃fat diet.After 11 weeks treatment with calcium chloride plus drinking water，the levels TC and blood sugar were significantlylowered，inflammatory factors IL⁃1β and IL⁃6 significantly decreased. IgM significantly increased，IgG significantlyincreased，and lipopolysaccharide significantly decreased in group D，as compared with group M. Calcium chlorideimproved the lipid disorders in the high⁃fat diet mice. Conclusion Calcium chloride can adjust the composition ofintestinal microbes so as to improve obesity in mice induced by high⁃fat diet.

Key words:

calcium chloride, hyperlipidemia, obesity, gut microbiota