Abstract:

Objective To investigate the mechanism of RBP2 gene silencing on the migration and invasion of the ovarian cancer cell line SKOV3/PTX. Methods The cell line that stably interferes with RBP2 expression was established，and the cells were divided into three groups：RBP2 silence（SKOV3/PTX⁃RBP2i），negative control （SKOV3/PTX⁃NC），and blank control（SKOV3/PTX）. Western blot was used to confirm the silencing effect of the RBP2 gene，wound healing assay was used to detect the migration ability of cells in three groups，transwell inva⁃ sion assay was used to detect the invasive ability of cells in three groups，and RT⁃PCR and western blot methods were used to detect molecular markers（β ⁃ catenin，VEGF and MMP ⁃ 2）. Results The cell line SKOV3/PTX ⁃ RBP2i，which stably inhibits RBP2 expression，was successfully constructed. The RBP2 expression level of silent group was significantly lower than that of negative control group and blank group. There were significant differences in wound healing rate and invasive ability among silent group，negative control group and blank group（P<0.01）. The wound healing rate and invasive ability of cells in silent group were significantly lower than those in negative control group and blank group（P<0.01）. RT ⁃PCR and western blot showed that the mRNA and protein expression levels of β⁃catenin，VEGF and MMP⁃2 in the silent group had significantly lower than those in the negative control group and the blank group（P<0.05）. Conclusion Interfering with RBP2 expression in SKOV3/PTX cells can prevent cell migration and invasion，and the mechanism is thought to be related to the Wnt/ β⁃catenin signaling pathway. 

Key words:

ovarian tumor, tumor, RBP2, migration, invasion