Abstract:

Objective To investigate the effect of fasudil combined with methylprednisolone sodium succi⁃nate on pulmonary fibrosis induced by paraquat poisoning. Methods Seventy⁃five Wistar male rats were randomlydivided into 5 groups，15 rats in each group：normal control group（group Cont），paraquatposoning group（groupPQ），methylprednisolone sodium intervention group（group MSS），fasudil intervention group（group FAS），meth⁃ylprednisolone sodium + fasudil intervention group（group MSS + FAS）. The daily status of rats in each group wasobserved and recorded. Five rats in each group were anesthetized and sacrificed on the 7th，14th and 21st day，respectively. The lung tissue samples were obtained. The histopathological changes of rats on the 21st day wereobserved by hematoxylin⁃eosin and Masson staining. The positive expression of transforming growth factor⁃β1（TGF⁃β1）in the lung tissue of rats in each group on the 21st day was determined by immunohistochemistry. The meanoptical density was measured. The content of TGF⁃β1 in the lung homogenates of rats in each group at each timepoint was determined by enzyme⁃inked immunosorbent assay. Results FAS and MSS could reduce the degree ofpulmonary fibrosis，FAS combined with MSS had better effect. Conclusion Fasudil combined with methylprednis⁃olone sodium succinate can reduce the degree of lung fiber induced by paraquat poisoning in rats

Key words: paraquat, pulmonary fibrosis, fasudil, methylprednisolone sodium, transforming growth factor?β1