HIF-1α三突变体对高糖、低氧环境下H9c2心肌细胞凋亡的影响

doi:10.3969/j.issn.1006-5725.2025.17.002

Abstract

Abstract:

Objective To investigate the effect of the mutant of adenovirus?mediated HIF?1α triple mutant (Ad?HIF?1α?Trip) on the apoptosis of H9c2 cardiomyocytes under high glucose and hypoxia conditions. Methods H9c2 cardiomyocytes were cultured under hypoxic conditions in vitro and randomly divided into four groups based on glucose concentration and viral transfection status： low oxygen + normal glucose concentration group （LO + NG group）， low oxygen + high glucose concentration group （LO + HG group）， low oxygen+high glucose + adenoviral null vector group （LO + HG + Ad?Null group）， and low oxygen+high glucose + adenovirus HIF?1α triple mutant group （LO + HG + Ad?HIF?1α?Trip group）. After 12 hours of hypoxia， HIF?1α， PI3K， and Akt expression were measured via qRT?PCR and Western blot， and apoptosis of H9c2 cardiomyocytes was assessed by flow cytometry. Results HIF?1α， PI3K， and Akt expression decreased in cardiomyocytes under high glucose and hypoxia， while Ad?HIF?1α?Trip enhanced their expression. Flow cytometry revealed increased apoptosis under high glucose and hypoxia， which was reduced by Ad?HIF?1α?Trip. Conclusion Ad?HIF?1α?Trip upregulates HIF?1α， PI3K， and Akt in H9c2 cardiomyocytes under high glucose and hypoxia， likely reducing apoptosis via PI3K?Akt pathway activation.

Key words: Ad?HIF?1α?Trip, adenovirus, PI3K?Akt signaling pathway, cardiomyocyte apoptosis, diabetes, diabetic cardiomyopathy

CLC Number:

R541.4

Li MAI,Yongting PAN,Wenkai HE,Xiaoqian WU,Mingyan LI. Effect of Ad⁃HIF⁃1α⁃Trip on apoptosis of H9c2 cardiomyocytes under high glucose and hypoxic conditions[J]. The Journal of Practical Medicine, 2025, 41(17): 2617-2623.

Figures/Tables 5

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References 28

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引物名称	引物序列
引物名称	前引物	后引物
β-actin	5′-GGCGAATACATAGATAC-3′	5′-GGTCTAATACCATTGA-3′
HIF-1α	5′-TCACAGCTCCTCAGCATTTC-3′	5′-GGACAAACTCCCTCACCAAA-3′
PI3K	5′-CCACTACCGGAATGAGTCTTTAG-3′	5′-CTGGTATTTGGACACTGGGTAG-3′
Akt	5′-GCTTCTATGGTGCGGAGATT-3′	5′-CTTGTCCAGCATGAGGTTCT-3′

Effect of Ad⁃HIF⁃1α⁃Trip on apoptosis of H9c2 cardiomyocytes under high glucose and hypoxic conditions

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