Abstract:

Objective To study the effect of Porphyromonas gingivalis （Pg） on the expression of exosomes （Ex） proteins secreted by Eca109 cells and their related functions and signal pathways. Methods The exosome proteins of Eca109 infected and uninfected by Pg were identified by Nano?LC?MS/MS. The differential proteins were selected by bioinformatics， and analyzed by GO enrichment and KEGG pathway. Results A total of 729 and 952proteins were identified in Pg?Ex and in Ex. Compared with Ex， 387 proteins were up?regulated and 294 proteins were down?regulated in Pg?Ex. These differential proteins are involved in biological processes such as cell proliferation， differentiation， adhesion， migration， angiogenesis，chemotaxis and immune response.They are enriched in Central Carbon Metabolism in tumor， PI3K?Akt signal pathway， HIF?1 signal pathway， Rap1 signal pathway，gap junction， Ras signal pathway， mTOR signal pathway， insulin signaling pathway， cGMP?PKG signaling pathway， ECM receptor interaction， chemokine signaling pathway， VEGF signaling pathway，etc. Conclusion It may indicate that Pg regulate tumor cell proliferation and migration， angiogenesis， immune response and thus participating in the development of esophageal cancer.

Key words: porphyromonas gingivalis, esophageal cancer, exosome, proteomics