血清lncRNA THRIL、lncRNA NEAT1与新生儿肺炎病情程度及预后的相关性

doi:10.3969/j.issn.1006-5725.2026.02.019

Abstract

Abstract:

Objective To investigate the relationship between serum long non-coding RNA tumor necrosis factor related heterologous ribonucleoprotein L （lncRNA THRIL） and long non-coding RNA nuclear enriched abundant transcript 1 （lncRNA NEAT1） and the severity and prognosis of neonatal pneumonia. Methods A total of 120 cases of neonatal pneumonia treated at the Fourth Hospital of Hebei Medical University from August 2022 to August 2024 were selected as the observation group. These cases were divided into the mild-disease group （42 cases）， the moderate-disease group （40 cases）， and the severe-disease group （38 cases） based on the severity of the disease. According to the prognosis after 2 weeks of treatment， the pediatric patients were classified into the good-prognosis group （86 cases） and the poor-prognosis group （34 cases）. Meanwhile， 120 healthy neonates who underwent physical examinations at the hospital during the same period were selected as the control group. The serum levels of lncRNA THRIL and lncRNA NEAT1 in the tested neonates were measured using real-time fluorescence quantitative polymerase chain reaction （PCR）. The clinical data of neonates with pneumonia were collected， and the immune inflammatory markers， namely soluble triggering receptor expressed on myeloid cells-1 （sTREM-1） and soluble interleukin-2 receptor （sIL-2R）， were detected. Logistic regression analysis was employed to identify and verify the influencing factors associated with poor prognosis in neonates with pneumonia. Considering the predictive effect of serum lncRNA THRIL and lncRNA NEAT1 on the poor prognosis of these children， receiver operating characteristic （ROC） curve analysis was conducted to evaluate the clinical value of each lncRNA individually and their combined value. Results The serum levels of lncRNA THRIL and lncRNA NEAT1 in the observation group were significantly higher than those in the control group （P < 0.05）. The serum levels of lncRNA THRIL and lncRNA NEAT1 gradually increased as neonatal pneumonia worsened （P < 0.05）. When compared with the good-prognosis group， the proportion of cesarean section and the levels of serum sTREM-1， sIL-2R， lncRNA THRIL， and lncRNA NEAT1 in the poor-prognosis group were significantly elevated （P < 0.05）. Serum sIL-2R， lncRNA THRIL， and lncRNA NEAT1 were identified as independent risk factors for poor prognosis in neonates with pneumonia （P < 0.05）. The area under the curve （AUC） values of serum lncRNA THRIL， lncRNA NEAT1， and their combination in predicting the poor prognosis of neonatal pneumonia were 0.772， 0.808， and 0.930， respectively. Moreover， the AUC of the combination of the two was significantly higher than that of each index alone （Z_{combination-lncRNA THRIL} = 2.347， Z_{combination-lncRNA NEAT1} = 2.217， P = 0.019， 0.027）. Conclusion Serum levels of lncRNA THRIL and lncRNA NEAT1 are significantly elevated in neonates with pneumonia. Both lncRNA THRIL and lncRNA NEAT1 serve as risk factors for the poor prognosis of neonatal pneumonia， and their combination exhibits a favorable predictive effect on the prognosis of neonates with pneumonia.

Key words: neonatal pneumonia, long non-coding RNA tumor necrosis factor related heterologous ribonucleoprotein L, long non-coding RNA nuclear enriched abundant transcript 1, severity of illness, prognosis

CLC Number:

R722.13⁺5

Xin LIU,Hongrui ZHANG,Ying SHEN,Yuqiao DIAO,Tao FAN. Correlation of serum lncRNA THRIL， lncRNA NEAT1 with the severity and prognosis of neonatal pneumonia[J]. The Journal of Practical Medicine, 2026, 42(2): 327-333.

Figures/Tables 8

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References 25

[1]	LIU M， MO J， NONG C， et al. Association between lung ultrasound score and PaO₂/FiO₂ ratio in neonatal pneumonia： A prospective cohort study［J］. J Clin Ultrasound， 2025， 53（4）：695-699. doi：10.1002/jcu.23928 . doi: 10.1002/jcu.23928
[2]	EL-SHEIKH M， ELMAHDY H， NASSAR M， et al. Role of soluble triggering receptors expressed on myeloid cells-1 and 25-hydroxy vitamin D as early diagnostic markers of neonatal Ventilator-associated pneumonia： A prospective cohort study［J］. Pediatr Pulmonol， 2022， 57（9）：2147-2153. doi：10.1002/ppul.26016 . doi: 10.1002/ppul.26016
[3]	SKAKUN O， VANDZHURA Y， VANDZHURA I， et al. Impact of age on predictive capabilities of ferritin， ferritin-hemoglobin ratio， IL-6， and sIL-2R for COVID-19 severity and mortality［J］. Acta Medica （Hradec Kralove）， 2024， 67（2）：53-59. doi：10.14712/18059694.2024.20 . doi: 10.14712/18059694.2024.20
[4]	NGUYEN T Q N， LE D Q， HOANG T M H， et al. Staphylococcus aureus pneumonia in neonates： Clinical patterns， laboratory findings and outcomes［J］. Pediatr Neonatol， 2025， 66（4）：390-395. doi：10.1016/j.pedneo.2025.03.002 . doi: 10.1016/j.pedneo.2025.03.002
[5]	JI J， HONG F， LIU Y， et al. Long noncoding RNA GAS5 contributes to mycoplasma pneumoniae pneumonia by regulating NF-κB via miR-29c/HMGB1 axis［J］. Tohoku J Exp Med， 2025， 264（4）：193-202. doi：10.1620/tjem.2024.J067 . doi: 10.1620/tjem.2024.J067
[6]	杨志勇，刘维娜. lncRNA THRIL在ICU脓毒症患者血清中的表达水平及临床价值［J］. 检验医学与临床， 2023， 20（1）：109-111. doi：10.3969/j.issn.1672-9455.2023.01.027 . doi: 10.3969/j.issn.1672-9455.2023.01.027
[7]	WANG X， LI W， SUN S， et al. Inter-correlation of lncRNA THRIL with microRNA-34a and microRNA-125b and their relationship with childhood asthma risk， severity， and inflammation［J］. Allergol Immunopathol （Madr）， 2023， 51（1）：187-194. doi：10.15586/aei.v51i1.736 . doi: 10.15586/aei.v51i1.736
[8]	GE S， HU J， GAO S， et al. LncRNA NEAT1： A novel regulator associated with the inflammatory response in acute respiratory distress syndrome［J］. Gene， 2023， 878：147582. doi：10.1016/j.gene.2023.147582 . doi: 10.1016/j.gene.2023.147582
[9]	郝淑娟，赵颖，马彤辉，等. 血清YKL-40、EGF表达水平与肺炎新生儿病情的关系及其对不良预后的预测价值分析［J］. 东南大学学报（医学版）， 2022， 41（5）：609-614. doi：10.3969/j.issn.1671-6264.2022.05.002 . doi: 10.3969/j.issn.1671-6264.2022.05.002
[10]	邵肖梅，叶鸿瑁，丘小汕. 实用新生儿学（第5版）［M］. 北京：人民卫生出版社， 2019： 575-578.
[11]	杨胜权，叶传芳，韩坤. 外周血sIL-2R、降钙素原、miR-223水平与新生儿肺炎病情及转归的关系［J］. 临床和实验医学杂志， 2024， 23（19）： 2089-2094. doi：10.3969/j.issn.1671-4695. 2024.1 9.019 . doi: 10.3969/j.issn.1671-4695. 2024.1 9.019
[12]	ARI M， ARI H F， CENGIZ H. Advanced biomarkers for prognostic evaluation of pneumonia severity in pediatric intensive care： Focus on novel inflammatory and hematological ratios［J］. Ital J Pediatr， 2025， 51（1）：168-180. doi：10.1186/s13052-025-01989-7 . doi: 10.1186/s13052-025-01989-7
[13]	LI C， FENG X， YANG L， et al. Clinical utility of the Platelet-to-neutrophil ratio in differentiating sepsis from neonatal pneumonia： An observational study［J］. Ann Med， 2025， 57（1）：2531252. doi：10.1080/07853890.2025.2531252 . doi: 10.1080/07853890.2025.2531252
[14]	BONDAREV D J， RYAN R M， MUKHERJEE D. The spectrum of pneumonia among intubated neonates in the neonatal intensive care unit［J］. J Perinatol， 2024， 44（9）： 1235-1243. doi：10.1038/s41372-024-01973-9 . doi: 10.1038/s41372-024-01973-9
[15]	KHODAIR H A， ELMAHDY H S， ZIDAN L K， et al. Assessment of the role of probiotics in prevention of ventilator-associated pneumonia in neonates［J］. Eur J Pediatr， 2025， 184（9）：597-606. doi：10.1007/s00431-025-06380-6 . doi: 10.1007/s00431-025-06380-6
[16]	SONG X， GAO F， LI H， et al. Long noncoding RNA THRIL promotes foam cell formation and inflammation in macrophages［J］. Cell Biol Int， 2023， 47（1）：156-166. doi：10.1002/cbin.11934 . doi: 10.1002/cbin.11934
[17]	SHI Q， LI Z， DONG Y， et al. LncRNA THRIL， transcriptionally activated by AP-1 and stabilized by METTL14-mediated m6A modification， accelerates LPS-evoked acute injury in alveolar epithelial cells［J］. Int Immunopharmacol， 2023， 123：110740. doi：10.1016/j.intimp.2023.110740 . doi: 10.1016/j.intimp.2023.110740
[18]	矫岩，石岩，韩文超，等. lncRNA THRIL和miR-300在新生儿败血症患者中的临床意义［J］. 中国妇幼健康研究， 2022， 33（8）：47-52. doi：10.3969/j.issn.1673-5293.2022.08.010 . doi: 10.3969/j.issn.1673-5293.2022.08.010
[19]	ALDAKHEEL F M， SYED R， AHMED M， et al. Modulation of lncRNA NEAT1 overturns the macrophages based immune response in M. tuberculosis infected patients via miR-373 regulation［J］. J Appl Genet， 2024， 65（2）：321-329. doi：10.1007/s13353-023-00808-1 . doi: 10.1007/s13353-023-00808-1
[20]	许云春，于馨雅，王雅芝，等. LncRNA NEAT1调控NF-κB信号通路对棕榈酸诱导的LO2细胞损伤与炎症反应的影响［J］. 实用医学杂志， 2023， 39（17）：2164-2170. doi：10.3969/j.issn.1006-5725.2023.17.003 . doi: 10.3969/j.issn.1006-5725.2023.17.003
[21]	GUO C， YU M， LIU J， et al. Molecular mechanism of Wilms tumour 1-associated protein in diabetes-related dry eye disease by mediating m6A methylation modification of lncRNA NEAT1［J］. J Drug Target， 2024， 32（2）：200-212. doi：10.1080/1061186X.2023.2300682 . doi: 10.1080/1061186X.2023.2300682
[22]	XU L， ZHANG L， XIANG Y， et al. Knockdown of lncRNA NEAT1 suppresses streptococcus pneumoniae-induced ferroptosis in alveolar epithelial cells by regulating the Nrf2-GPX4 pathway［J］. Toxicon， 2024， 243：107705. doi：10.1016/j.toxicon. 2024. 107705 . doi: 10.1016/j.toxicon. 2024. 107705
[23]	屈菊梅，冯成虎，曹贝贝. 急性呼吸窘迫综合征新生儿血清LncRNA NEAT1、miR-129-5p水平与病情程度及预后的关系［J］. 中国妇幼健康研究， 2025， 36（5）：31-37. doi：10.3969/j.issn.1673-5293.2025.05.005 . doi: 10.3969/j.issn.1673-5293.2025.05.005
[24]	XU C， LUO L Y， WU B C， et al. Diagnostic values of soluble triggering receptor expressed on myeloid cells （sTREM-1） and interferon-inducible protein-10 （IP-10） for severe mycoplasma pneumoniae pneumonia in children［J］. Clinics （Sao Paulo）， 2024， 79： 100361. doi：10.1016/j.clinsp.2024.100361 . doi: 10.1016/j.clinsp.2024.100361
[25]	SCHUMACHER L， MÜLLER A， KOCH A， et al. Increased serum soluble interleukin-2 receptor concentrations are linked to high-sensitivity troponin T and disease progression in systemic sclerosis［J］. Clin Exp Rheumatol， 2025， 43（8）：1446-1454. doi：10.55563/clinexprheumatol/re755k . doi: 10.55563/clinexprheumatol/re755k

引物名称	引物序列
lncRNA THRIL	上游引物：5′-AACTTCACAGGAACACTACACAAGA-3′
	下游引物：5′-TAGGCAACAGAGCAAGACTTCATC-3′
lncRNA NEAT1	上游引物：5′-TGTCCCTCGGCTATGTCAGA-3′
	下游引物：5′-GAGGGGACGTGTTTCCTGAG-3′
甘油醛-3-磷酸脱氢酶	上游引物：5′-GAGTCCACTGGCGTCTTCAC-3′
	下游引物：5′-ATCTTGAGGCTGTTGTCATATTCT-3′

组别	例数	lncRNA THRIL	lncRNA NEAT1
对照组	120	1.12 ± 0.27	1.05 ± 0.12
观察组	120	2.28 ± 0.49	2.22 ± 0.51
t值		22.713	24.463
P值		< 0.001	< 0.001

组别	例数	lncRNA THRIL	lncRNA NEAT1
轻症组	42	1.51 ± 0.29	1.12 ± 0.34
中症组	40	2.35 ± 0.45^?	2.21 ± 0.48^?
重症组	38	3.05 ± 0.76^?#	3.46 ± 0.76^?#
F值		85.161	182.103
P值		< 0.001	< 0.001

项目	预后良好组（n = 86）	预后不良组（n = 34）	t/χ² 值	P值
日龄（x ± s）/d	12.66 ± 2.37	12.34±2.23	0.677	0.499
性别			0.859	0.354
男	51（59.30）	17（50.00）
女	35（40.70）	17（50.00）
出生体质量			2.116	0.146
< 3 kg	33（38.37）	18（52.94）
≥ 3 kg	53（61.63）	16（47.06）
出生季节			0.119	0.730
春秋季	46（53.49）	17（50.00）
冬夏季	40（46.51）	17（50.00）
分娩方式			7.694	0.006
顺产	59（68.60）	14（41.18）
剖腹产	27（31.40）	20（58.82）
病因			0.479	0.489
吸入性肺炎	49（56.98）	17（50.00）
感染性肺炎	37（43.02）	17（50.00）
sTREM-1（x ± s）/（ng/L）	86.77 ± 19.48	103.52 ± 20.51	4.181	< 0.001
sIL-2R（x ± s）/（ng/L）	328.62 ± 51.36	375.44 ± 57.65	4.345	< 0.001

组别	例数	lncRNA THRIL	lncRNA NEAT1
预后良好组	86	1.98 ± 0.34	1.93 ± 0.42
预后不良组	34	3.04 ± 0.87	2.96 ± 0.74
t值		9.635	9.605
P值		< 0.001	< 0.001

Correlation of serum lncRNA THRIL， lncRNA NEAT1 with the severity and prognosis of neonatal pneumonia

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指标	β	SE	Wald	P值	OR	95%CI
sIL-2R	0.670	0.262	6.537	0.010	1.954	1.169 ~ 3.265
lncRNA THRIL	0.716	0.353	4.113	0.043	2.046	1.024 ~ 4.087
lncRNA NEAT1	0.868	0.416	4.349	0.037	2.381	1.054 ~ 5.381

变量	AUC	截断值	95%CI	敏感度/%	特异度/%	约登指数
lncRNA THRIL	0.772	2.40	0.686 ~ 0.844	68.00	90.53	0.585
lncRNA NEAT1	0.808	2.61	0.726 ~ 0.874	70.83	93.48	0.643
两者联合	0.930		0.869 ~ 0.969	91.89	88.66	0.806

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