Abstract:

Objective To observe the improvement effect of ginsenoside Rb1（G⁃Rb1）on lung injury of neonatal rats induced by hyperoxia，and to explore its mechanism. Method Hyperoxic lung injury model of neonatal rats was established in model group，Rb1 group，and Rb1 combined with ML385 group. Rb1 combined with ML385 group was intraperitoneally injected with G⁃Rb1（10 mg/kg）+ intragastric ML385（20 mg/kg）；Rb1 group with G⁃Rb1 （10 mg/kg）+ gavage the same amount of normal saline and the blank group and model group with the same amount of normal saline + gavage with the same amount of normal saline. The activity of malondialdehyde（MDA）and superoxide dismutase（SOD），the content of lung surface active substance protein⁃D（SP⁃D）and the expression of Nrf2 protein in lung tissue were detected. Results Compared with those in model group，MDA level and SP⁃D level in Rb1 group were decreased，but SOD activity and Nrf2 protein expression were increased（P < 0.05）. Compared with those in Rb1 group，MDA level，and SP ⁃D level in Rb1 combined with ML385 group were increased，but SOD activity，and Nrf2 protein expression were decreased（P < 0.05）. Conclusion G⁃Rb1 can improve lung injury of newborn mice，enhance the body′s antioxidant capacity，and inhibit the oxidative stress response of lung tissue. Activation of the Nrf2/ARE pathway may be one of its mechanisms.

Key words:

ginsenoside Rb1, hyperoxia, newborn rats, lung injury