The Journal of Practical Medicine ›› 2022, Vol. 38 ›› Issue (23): 2908-2913.doi: 10.3969/j.issn.1006⁃5725.2022.23.004

• Basic Research • Previous Articles     Next Articles

Long non⁃coding RNA LINP1 inhibits the DNA damage induced by ionizing radiation and promote radio⁃ resistance in non⁃small cell lung cancer

ZHANG Chunting，LIANG Xiaoting，WANG Le，ZHOU Liang.   

  1. Depart⁃ ment of Toxicology，School of Public Health，Southern Medical University（Guangdong Provincial Key Laboratory of Tropical Disease Research），Guangzhou 510515，China

  • Online:2022-12-10 Published:2022-12-10
  • Contact: ZHOU Liang E⁃mail：zhzliang@smu.edu.cn

Abstract:

Objective To explore the effect of long non⁃coding RNA LINP1 on ionizing radiation⁃induced DNA damage in non⁃small cell lung cancer（NSCLC）and provide theoretical basis for novel combination treatment option. Methods H1299 NSCLC cells were transfected with siRNAs.CCK ⁃ 8 assays and colony formation assays were used to check for H1299 cell growth after 8.0 Gy of ionizing radiation.To find the DNA damage in H1299 cells，Western blot and comet assay were used. Results After receiving ionizing radiation（IR），LINP1 was sig⁃ nificantly（P < 0.001）upregulated in H1299 cells. Compared with the control，the proliferation capacity and clon⁃ al formation ability decreased（P < 0.001），while the DNA damage（P < 0.001）was increased significantly（P < 0.001）. Under 8.0 Gy IR treatment，LINP1 knockdown significantly reduced H1299 cell proliferation and clonal formation ability（P < 0.01），as well as DNA damage repair ability（P < 0.001）. Conclusions LINP1 inhibits proliferation and increases IR⁃induced DNA damage in NSCLC cells，while also promoting radioresistance.

Key words:

long non?coding RNA, LINP1, non?small cell lung cancer, DNA damage, radioresis? tance