Abstract:

Objective To investigate the effect of long⁃stranded non⁃coding RNA（LNCRNA）Linc00858on the proliferation，migration and invasion of hepatoma cells by regulating targeted small molecule RNA⁃3126⁃5P（MIR⁃3126⁃5P）. Methods Real⁃time fluorescent quantitative PCR（qRT⁃PCR）was used to detect the expressionsof Linc00858 in hepatocellular carcinoma（HCC），normal para ⁃ cancerous tissues，normal human hepatocytesLO2 and human hepatoma cell lines. Human hepatoma cell lines HUH ⁃ 7 and MHCC ⁃97H were transfected withLinc00858⁃overexpressed plasmid and Linc00858⁃expressing recombinant lentivirus，respectively. The transfectioneffect was determined by qRT⁃PCR，and proliferation of tumor cells was detected by CCK⁃8 and Transwell assay.Western Blot assay was used to determine the effects of link00858 on epithelial⁃mesenchymal transformation and expression of cell cycle related proteins. Bioinformatics and double luciferase assay were used to determine the association of Linc00858 with miR⁃3126⁃5p. After the level of Linc00858 expression was adjusted，the expressionof miR ⁃3126⁃5p in hepatoma cells was detected by qRT PCR. miR ⁃3126⁃5p mimics and miR ⁃3126⁃5p inhibitorwere transfected respectively；then the proliferation，migration，invasion and expression of related proteins wereanalyzed by the above methods. Results Linc00858 expression was higher in cancerous tissues than in LO2 andnormal tissues. Proliferation，migration and invasion of HUH ⁃ 7 cells were increased after overexpression ofLinc00858. The protein expressions of Vimentin，Cyclin E1 and CDK2 increased obviously，while those of E⁃cad⁃herin and P27 decreased obviously. Proliferation，migration and invasion of MCHH⁃97H cells were inhibited bysilencing Linc00858. The expression levels of Cyclin E1，CDK2 and Vimentin ⁃ related proteins decreased signifi⁃cantly，while those of E ⁃cadherin and P27 increased significantly. Linc00858 regulated miR ⁃3126⁃5p expression，miR ⁃ 3126 ⁃ 5p mimics significantly reversed proliferation，migration and invasion of HUH ⁃ 7 cells induced byLinc00858 overexpression. miR ⁃3126⁃5p inhibitor reversed the impact of Linc00858 silencing on proliferation，migration and invasion ability of MHCC⁃97H cells and related protein expression level. Conclusions Linc00858accelerated proliferation，invasion and migration of hepatoma cells through the negative regulation of microR⁃NA3126⁃5p.

Key words:

hepatocellular carcinoma, long?stranded non?coding RNA 00858, small molecule RNA?3126?5p, cellular invasion, cellular migration