Abstract:

Objective To explore the effect of TRPV1 on blood⁃brain barrier injury and anxiety⁃like behaviors after cerebral ischemia⁃reperfusion in mice and its and mechanism. Methods A model of middle cerebral artery embolism（MCAO）was constructed by use of thread embolization. Capsazepine（CPZ）of 7.5 μg/3 uL was injected into the lateral ventricle. HE staining was used to detect pathological changes in amygdala；Immunofluores⁃ cence staining was applied to detect vascular damage in amygdala；Western blot was used to detect levels of p⁃JNK and p ⁃ p38 MAPK；High plus maze was used to detect anxiety ⁃like behaviors. Results As compared with I/R group，the moving distance and length of stay in the open arms of high plus maze in I/R+CPZ group increased significantly（P < 0.001）. HE staining showed that nucleus pyknosis and edema，as compared with I/R group， were reduced in I/R + CPZ group. As compared with I/R group，Claudin5 expression was increased in I/R + CPZ group，whereas levels of p⁃JNK and p⁃p38 MAPK were significantly reduced（P < 0.05）. Conclusions Inhibition of TRPV1 can alleviate damage in blood⁃brain barrier and anxiety⁃like behaviors after cerebral ischemia in mice by reducing levels of p⁃JNK and p⁃p38 MAPK.

Key words:

TRPV1, brain ischemia, blood brain barrier, anxiety