Abstract:

Objective To evaluate the expression of tissue factor（TF）on platelet surface during prepara⁃ tion and storage of platelet concentrates（PC）for transfusional use. Methods Platelet TFs in healthy donor whole blood and PC were measured by flow cytometry on the day of preparation and up to 4 days of storage with classical platelet activation markers，i.e.，fibrinogen，P ⁃selectin，and glycoprotein GPIIb（CD41）analyzed as well. The data were analyzed according to blood ABO group. Results TFs were detected on whole blood platelets，with the highest detection ratein O donors. Compared to the whole blood，the detected TFs were higher on the platelet surface，even escalating with the process of storage. The rise in TF levels were positively correlated with the elevations of the other platelet markers. Conclusion Platelet surface TF is contained in PC. Further studies are needed to investigate a possible correlation between the TF levels and the hemostatic response of platelet transfusion recipient.

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