The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (5): 660-665.doi: 10.3969/j.issn.1006⁃5725.2021.05.020

• Medical Examination and Clinical Diagnosis • Previous Articles     Next Articles

Diagnostic value of granzyme B stimulated by ESAT ⁃ 6/CFP ⁃ 10 in pediatric patients with tuberculosis

SUN Na*，YAO Cong，SHEN Xin，WANG Jun，YUAN Chunhui，XIANG Yun.   

  1. Wuhan Children′s Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430016，China

  • Online:2021-03-10 Published:2021-03-10
  • Contact: XIANG Yun E⁃mail：xiangyun5272008@163.com

Abstract:

Objective To explore the diagnostic value of granzyme B stimulated by ESAT⁃6/CFP⁃10 in pediatric patients with tuberculosis. Methods A total of 86 children（26 children with ATB，20 with LTBI，20 Non⁃TB，20 healthy）from Wuhan Jinyintan Hospital and Wuhan Children′s Hospital from January to August 2019 were selected. ELISA was used to detect the level of GrB in peripheral blood after ESAT⁃6/CFP⁃10 stimulation and flow cytometry to detect the expression of GrB and IFN⁃ γ in CD4+ /CD8+ T cells. Results After ESAT⁃6/CFP⁃10 stimulation，the level of MTB⁃specific GrB of tuberculosis patients was significantly increased when compared with the control group（P < 0.001），especially，the level of MTB⁃specific GrB of LTBI patients was notably higher that of ATB patients（P < 0.001）. GrB can be secreted jointly by CD4+ T and CD8+ T cells. Conclusion MTB⁃specific GrB may have great value in the differential diagnosis of active tuberculosis in pediatric patients.

Key words: