The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (5): 620-625.doi: 10.3969/j.issn.1006⁃5725.2021.05.013

• Clinical Research • Previous Articles     Next Articles

Proliferation，migration and invasion of glioma pancreatic carcinoma regulated by LncRNA PCAT6 via targeting miR ⁃ 185 ⁃ 5p

HAO Yuqiao，LIU Lang，WANG Jian′ gang，GUAN Canghai，WANG Dongsheng， ZHONG Xiangyu，JIANG Xingming   

  1. Department of General Surgery，the Second Affiliated Hospital of Harbin Medi⁃ cal University，Harbin 150086，China

  • Online:2021-03-10 Published:2021-03-10
  • Contact: JIANG Xingming E⁃mail：jiangxingming@hrbmu.edu.cn

Abstract:

Objective To investigate the expression of long noncoding RNA（lncRNA）prostate cancer associated transcript 6（PCAT6）in pancreatic carcinoma tissues and cells and its molecular mechanism of regulating proliferation ，migration and invasion in pancreatic carcinoma cells. Methods The PCAT6 and miR ⁃185⁃5p expression in pancreatic carcinoma tissues，paired adjacent normal tissues and pancreatic carcinoma cells was detected by qRT⁃PCR. CCK8，Transwell assay was used to detect the ability proliferation，migration and invasion of pancreatic carcinoma cells. Bioinformatics prediction and dual luciferase reporter gene assay were used to demon⁃ strate the targeting relationship between PCAT6 and miR⁃185⁃5p. Results PCAT6 was up⁃regulated in pancreatic carcinoma compared with adjacent normal tissue and over⁃expression of PCAT6 was correlated with advanced TNM stage（P < 0.05）and lymph node invasion（P < 0.05）. The exogenous silencing of PCAT6 could inhibit the prolif⁃ eration，migration and invasion of pancreatic carcinoma cells. The activity of luciferase significantly reduced after co⁃transfection of PCAT6⁃WT and miR⁃185⁃5p mimics（P < 0.05），but there was no obviously change of luciferase activity when PCAT6⁃WUT and miR⁃185⁃5p mimics co⁃transfected. Conclusion LncRNA PCAT6 regulates prolif⁃eration，migration and invasion of pancreatic carcinoma cells by targeting miR⁃185⁃5p.

 

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