Abstract:

Objective To investigate the protective effect of Hui⁃Hui Gan⁃song Yin（HGY）on the glomer⁃ ulus of diabetic nephropathy（DN）in rats. Methods Ten rats were randomized into the normal control group （NC）. The early DN models of SD rats were reproduced by high fat feed combined with streptozotocin. The rat DN models were randomly divided into DN model groups（DN），Glenn sinensis group（DN + Gli，10 mg/kg），spikenard drink high dose group（DN + HH，10 g/kg），and spikenard drinking low dose group（DN + HL，5 g/kg），10 rats in each group. MRNA expression of miR⁃192，the target genes of Zeb2 and TGF⁃β1，Smad3 and Smad7 in renal tissue was detected by real⁃time fluorescence quantitative PCR. Western blot was used to detect the protein expres⁃ sion of the above genes. Results Compared with the blank control group，the mRNA levels of TGF⁃β1，Smad3 and miR⁃192 in the renal tissue of the DN model group was significantly increased（P < 0.01，respectively），while the mRNA expression of Smad7，S1P1 and Zeb2 and Smad7 was significantly decreased（P < 0.01，respectively）. Compared with the DN model group，the mRNA levels of TGF⁃β1，miR⁃192 and Smad3 were significantly decreased （P < 0.01，respectively），and the mRNA expression of Smad7，S1P1，Zeb2 and Smad7 was significantly increased after the treatment of HGY. Conclusion HGY may improve the accumulation of extracellular matrix in the kidney of DN rats by regulating miR⁃192⁃mediated TGF⁃β1/Smads signaling pathway，contributing to protecting the kid⁃ 

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