Abstract:

Objective To investigate the role of lncRNA H19 in pyrophosis of glomerular podocyte induced by high glucose（HG）. Methods The background expression of lncRNA H19 in HG was detected by qRT PCR；western blot was used to detect the expression of Nephrin and Podocin；detection of NLRP3，Caspase⁃1 and IL⁃1β expression level was performed by using western blot and immunofluorescence. QRT⁃PCR was used to detect the mRNA expression of lncRNA H19 after transfection of Ad lncRNA H19 and si lncRNA H19. The cell transfec⁃ tion were set as follows：（1）control group（without treatment），negative control group of lncRNA H19 overexpres⁃ sion，lncRNA H19 overexpression group；（2）control group（without treatment），negative control group of lncRNA H19 interference and lncRNA H19 interference group（si lncRNA H19⁃1，si lncRNA H19⁃2，si lncRNA H19⁃3）. Detection of NLRP3，Caspase⁃1 and IL⁃1βexpression level after transfection with Ad lncRNA H19 and si lncRNA H19 was performed by western blot. The cell transfection were set as follows：（1）control group（without treatment）high glucose group，lncRNA H19 overexpression control+high glucose group，lncRNA H19 overexpression+high glucose group，（2）control group（without treatment），high glucose group，lncRNA H19 interference control+high glucose group，and lncRNA H19 interference+high glucose group. Results （1）Compared with that in the control group（without treatment），the expression of lncRNA H19 was low in the process of renal podocyte injury induced by high glucose（P < 0.05），and the low expression of lncRNA H19 led to higher expression of pyrophosis⁃related proteins NLRP3，Caspase⁃1，and IL⁃1 β. There was a negative correlation between the expression of lncRNA H19 and the expression of pyrophosis⁃related proteins.（2）Compared with that in the control group（without treatment）， the expression level of Nephrin and Podocin in renal podocytes decreased during the process of injury induced by high glucose（P < 0.05）.（3）Compared with that in the lncRNA H19 overexpression control+ high glucose group， overexpression of lncRNA H19 alleviated high glucose induced nephropodocyte pyrophosis，and the expression of pyrophosis⁃related proteins NLRP3，Caspase⁃1，and IL⁃1 β was low（P < 0.05）.（4）Compared with that in the lncRNA H19 interference control+high glucose group，the interference of lncRNA H19 promoted the high glucose⁃ induced podocyte pyrophosis，while the pyrophosis⁃related proteins NLRP3，Caspase⁃1，and IL⁃1 β were highly expressed（P < 0.05）. Conclusion In the process of podocyte pyrophosis induced by high glucose，lncRNA H19 can regulate the expression of pyrophosis⁃related proteins，which provides theoretical basis for the treatment of dia⁃ betic nephropathy in the future.

Key words:

LncRNAH19, diabetic nephropathy, renal podocyte damage, cell scorch