Abstract:

Objective To conduct a focused investigation on the effect of the TFAM gene on the proliferation and invasion of renal cancer cells. Methods Renal cancer cells ACHN and A704 were transfected with a plasmid for TFAM overexpression， and cell apoptosis was detected by flow cytometry. In ACHN and A704 cells， TFAM was knocked down， and cell proliferation， cell cycle， and invasion ability were assessed using CCK8， flow cytometry， invasion， and migration assays. Changes in mRNA and protein levels after TFAM overexpression and knockdown were detected by qPCR and western blot， respectively. qPCR and western blot were used to detect mRNA and protein changes after TFAM overexpression and knockdown， and ChIP-qPCR was performed to explore TFAM target genes. Results After transfection with the plasmid， ACHN and A704 cells exhibited a significant increase in TFAM expression compared to the control group， and the apoptosis of cells in the high TFAM expression group was significantly reduced. When TFAM was knocked down in ACHN and A704 cells， TFAM levels were significantly lower than those in the control group， and the proliferation and invasion abilities of cells with low TFAM expression were significantly decreased compared to the control group. High expression of TFAM enhanced the proliferation ability of renal cancer cells， while low expression of TFAM inhibited the proliferation and invasion abilities of the cells. TFAM can target and regulate CDC25B and CCNE2， and it may promote renal cancer cell proliferation by activating CDC25B and CCNE2. Conclusion TFAM can promote the proliferation and invasion of renal cancer cells.

Key words: transcription factor A, mitochondrial, renal cancer cells, proliferation, invasion