Abstract:

Objective To investigate the in vitro anti-inflammatory effects of Sophora davidii Hance leaves total alkaloids（SDLTAs）and possible molecular mechanisms. Methods The lipopolysaccharide （LPS）-induced inflammation model of RAW264.7 cells was used， and different concentrations of SDLTAs （50， 100 and 200 μg/mL） were administered， and the effect of SDLTAs on cellular NO expression was detected by the Griess method； ELISA method was used to detect the effect of SDLTAs on the expression of IL-6， TNF-α and IL-1β； The expression of iNOS， NF- κB p65 and IκBα mRNA was detected by RT-qPCR； Western blotting was used to detecte the expression of p-p38， p-p65 and p-JNK in the cells and NF- κB p65 in the nucleus. Results SDLTAs could significantly inhibit the LPS-induced inflammatory response in RAW264.7 cells. SDLTAs significantly decreased the secretion of NO， IL-6， TNF-α and IL-1β in cells （P < 0.01）， and significantly decreased the mRNA expressions of iNOS， NF-κB p65 and IκBα in cells （P < 0.01）. Significantly decreased the protein expression of p-p38， p-p65 and p-JNK in cells and NF-κB p65 in nucleus （P < 0.01）. Conclusion SDLTAs can exert anti-inflammatory effects by regulating the MAPK/NF-κB signalling pathway.

Key words: Sophora davidii （Franch.） Skeels, total alkaloid, anti-inflammation, action mechanism