富白细胞-血小板纤维蛋白在大鼠牙髓修复中的应用

doi:10.3969/j.issn.1006-5725.2024.14.007

Abstract

Abstract:

Objective To explore the effect of leukocyte-and platelet-rich fibrin （L-PRF） as a capping agent in the modulation of inflammation in rat dental pulp. Methods The rat model of pulpitis was established by lipopolysaccharide（LPS）. A total of 30 Sprague-Dawley rats were randomly divided into five groups： Normal control group， Model group， iRoot BP PLUS group， L-PRF group， iRoot BP PLUS+ L-PRF group. Samples were collected on the 14th day after pulp capping. Micro-CT scans， HE and Masson staining，qRT-PCR assays were performed to observe pulp tissue inflammatory and hard tissue formation. Results Micro-CT results： no hard tissue formation was seen in the LPS group， plaque calculus was seen in the L-PRF group， and a more complete calcified bridge was seen at the perforation of the pulp in the BP and combined groups； HE and Masson staining results： the LPS group had the most severe inflammation and no hard tissue formation； the combined group had the least inflammation and the most complete and continuous hard tissue formation， and there was a statistical difference between the two groups（P < 0.01）； There was no statistical difference in the degree of inflammatory reaction and hard tissue formation between the BP group and the combined group （P > 0.05）；qRT-PCR results： the relative expression levels of TNF-α， IL-1β， and IL-6 mRNA in the pulp tissues of all experimental groups were reduced compared with those of the LPS group， but the differences between the L-PRF and LPS groups were not statistically significant （P > 0.05）. Conclusion The combination of L-PRF and iRoot BP PLUS as a composite pulp capping material can be used for the repair of dental pulp tissue and the regulation of inflammation in dental pulpitis in rats.

Key words: leukocyte-and platelet-rich fibrin, pulpitis, direct pulp capping, inflammatory response, reparative dentin

CLC Number:

R781.31

Ting WANG,Li. HE. Application of leukocyte⁃and platelet⁃rich fibrin in dental pulp repair of rat[J]. The Journal of Practical Medicine, 2024, 40(14): 1941-1946.

Figures/Tables 6

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References 23

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组别	炎症细胞评分				平均秩	P值	硬组织形成评分				平均秩	P值
组别	1	2	3	4	平均秩	P值	1	2	3	4	平均秩	P值
LPS组	0	0	1	5	20.08	< 0.001	0	0	0	6	20.50	< 0.001
L?PRF组	0	1	3	2	16.83		0	0	4	2	16.50
BP组	5	1	0	0	7.08		5	1	0	0	7.00
联合组	6	0	0	0	6.00		6	0	0	0	6.00

组别	牙髓炎症反应程度				硬组织形成程度
组别	LPS组	L?PRF组	BP组	联合组	LPS组	L?PRF组	BP组	联合组
LPS组	-	1.000	0.004^*	0.001^*	-	1.000	0.002^*	0.001^*
L?PRF组	1.000	-	0.064	0.027^*	1.000	-	0.074	0.034^*
BP组	0.004^*	0.064	-	1.000	0.002^*	0.074	-	1.000
联合组	0.001^*	0.027^*	1.000	-	0.001^*	0.034^*	1.000	-

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Application of leukocyte⁃and platelet⁃rich fibrin in dental pulp repair of rat

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