基于西洋参总皂苷调控TGF-β1/Smad信号通路对压力性尿失禁大鼠模型盆底功能恢复的影响

doi:10.3969/j.issn.1006-5725.2023.19.007

Abstract

Abstract:

Objective To explore the effect of TGF-β1/Smad signaling pathway regulated by total saponins of American ginseng on the functional recovery of pelvic floor in rats with stress urinary incontinence. Methods Sixty-five SD rats of SPF grade were selected， and 45 rats after modeling were randomly divided into model group， low-dose group and high-dose group with 15 rats each， and the remaining 15 rats without any treatment were used as control group. Rats in low-dose group and high-dose group were given total saponins of Panax quinquefolium according to the dosage of 50 mg/kg and 100 mg/kg respectively， while rats in control group and model group were given the same amount of physiological saline （15 mL）. The pathological histology， pelvic floor function related indexes， urodynamics related indexes， bladder internal pressure related indexes， pelvic floor muscle surface electrical signals and the relative expression of TGF-β1/Smad signaling pathway protein were compared in each group. Results Compared with those in the control group， urethra rotation angle， bladder neck motion， residual urine volume， urine output， Smad2 and Smad3 expression were increased in the model group， the low and high dose group； vaginal resting pressure， systolic pressure， LPP， MBC， ALPP， urinary efficiency， Class Ⅰ and Class Ⅱ pelvic floor muscle fiber potential， TGF-β1， and Smad7 level were decreased （P < 0.05）； Compared with those in the model group， vaginal resting pressure， systolic blood pressure， LPP， MBC， ALPP， urinary efficiency， class Ⅰ and Class Ⅱ pelvic floor muscle fiber potential， TGF-β1， Smad7 expression increased in the low and high dose group； urethral rotation angle， bladder neck motion， residual urine volume， urine output， Smad2 and Smad3 levels were decreased （P <0.05）. Conclusion American ginseng total saponin could effectively improve pelvic floor function in rats with stress urinary incontinence， and the mechanism may be related to TGF- β1/Smad signaling pathway.

Key words: stress incontinence, total saponin, transforming growth factor-β1/Smad, pelvic floor function

CLC Number:

R361

Lu LI,Xiujuan. LI. Effect of TGF-β/Smad signaling pathway regulated by total saponins of American ginseng on functional recovery of pelvic floor in rats with stress urinary incontinence[J]. The Journal of Practical Medicine, 2023, 39(19): 2450-2455.

Figures/Tables 7

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References 20

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组别	n	阴道静息压（cmH₂O）	阴道收缩压（cmH₂O）	尿道旋转角度（°）	膀胱颈活动度（cm）
对照组	15	56.17 ± 6.65	68.91 ± 7.86	31.53 ± 4.10	0.69 ± 0.10
模型组	15	32.76 ± 4.03^a	35.27 ± 5.12^a	49.43 ± 6.49^a	1.30 ± 0.27^a
低剂量西洋参总皂苷组	15	39.74 ± 5.23^ab	44.75 ± 6.37^ab	43.31 ± 5.54^ab	1.19 ± 0.19^ab
高剂量西洋参总皂苷组	15	48.42 ± 5.93^abc	56.47 ± 6.88^abc	37.89 ± 4.87^abc	0.92 ± 0.13^abc
F值		11.660	13.890	9.031	8.205
P值		0.001	0.001	0.001	0.001

组别	n	LPP（mmHg）	MBC（mL）	ALPP（mmHg）
对照组	15	38.13 ± 6.51	6.21 ± 1.04	41.16 ± 6.08
模型组	15	12.37 ± 4.43^a	1.16 ± 0.21^a	13.43 ± 4.54^a
低剂量西洋参总皂苷组	15	20.26 ± 5.26^ab	3.15 ± 0.34^ab	21.34 ± 5.15^ab
高剂量西洋参总皂苷组	15	29.35 ± 5.94^abc	4.77 ± 0.62^abc	35.24 ± 5.76^abc
F值		12.670	18.430	14.150
P值		0.001	0.001	0.001

组别	n	残余尿量（mL/d）	排尿量（mL/d）	排尿效率（%）
对照组	15	0.76 ± 0.20	54.27 ± 10.15	91.33 ± 14.50
模型组	15	3.17 ± 1.10^a	133.32 ± 15.61^a	32.82 ± 11.25^a
低剂量西洋参总皂苷组	15	2.38 ± 0.92^ab	105.11 ± 13.47^ab	56.13 ± 12.31^ab
高剂量西洋参总皂苷组	15	1.48 ± 0.65^abc	83.87 ± 12.30^abc	70.77 ± 13.45^abc
F值		8.348	16.440	12.350
P值		0.001	0.001	0.001

组别	n	Ⅰ类盆底肌纤维电位（mV）	Ⅱ类盆底肌纤维电位（mV）
对照组	15	17.23 ± 3.35	19.16 ± 4.27
模型组	15	8.26 ± 1.67^a	10.30 ± 1.72^a
低剂量西洋参总皂苷组	15	11.74 ± 2.52^ab	13.07 ± 2.78^ab
高剂量西洋参总皂苷组	15	13.30 ± 3.01^abc	15.67 ± 3.44^abc
F值		9.281	7.545
P值		0.001	0.001

组别	TGF-β1	Smad2	Smad3	Smad7
对照组	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01
模型组	0.42 ± 0.08^a	3.42 ± 0.25^a	3.25 ± 0.21^a	0.39 ± 0.07^a
低剂量西洋参总皂苷组	0.69 ± 0.010^ab	2.50 ± 0.19^ab	2.33 ± 0.16^ab	0.65 ± 0.09^ab
高剂量西洋参总皂苷组	0.85 ± 0.012^abc	1.68 ± 0.11^abc	1.59 ± 0.10^abc	0.83 ± 0.011^abc
F值	27.860	37.480	41.450	33.410
P值	0.001	0.001	0.001	0.001

Effect of TGF-β/Smad signaling pathway regulated by total saponins of American ginseng on functional recovery of pelvic floor in rats with stress urinary incontinence

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