Abstract:

Objective To explore the effects of miR⁃496/DCUN1D1 axis on cervical cancer development to reveal the role of miR⁃496 in cervical cancer. Methods Immunofluorescence and Western blot were used to detect DCUN1D1 expression in cervical cancer and normal adjacent tissues. QRT ⁃PCR was used to detect the expression of DCUN1D1 and miR ⁃496 in cervical cancer tissues of different stages. Target gene of miR ⁃ 496 was screened and verified. qRT⁃PCR and Western blot were used to detect the effects of miR⁃496 mimics on DCUN1D1 and CCK8 assay and Transwell the effects of miR⁃496 and DCUN1D1 on HeLa cell proliferation，migration and invasion. A nude mouse xenograft model of cervical cancer was constructed to examine the effects of miR⁃496 on tumor growth. Results The expression of miR⁃496 was lower in the cervical cancer tissues than in the para⁃carci⁃ noma tissues. The higher the clinical stage of cervical cancer，the higher the mRNA expression of DCUN1D1 and the lower the expression of miR⁃496（P < 0.05）. miR⁃496 could inhibit DCUN1D1 expression via binding to 3′⁃UTR of DCUN1D1. Protein and mRNA expression of DCUN1D1 in the miR ⁃496⁃overexpressed cells were significantly decreased（P < 0.05）. The proliferation，migration and invasion of HeLa cells were more significantly suppressed in miR⁃496 group than in the control group. The migration and invasion of HeLa cells were more significantly enhanced in the miR⁃496+DCUN1D1 group than in the mir⁃496 group（P < 0.05）. In the xenograft model of nude mice，miR⁃ 496 could down⁃regulate DCUN1D1 and inhibit the tumor growth（P < 0.05）. Conclusion Abnormal expression of miR ⁃496 and DCUN1D1 is related to the pathogenesis of cervical cancer，and miR ⁃496 suppresses the occur⁃ rence and development of cervical cancer by targeting DCUN1D1.

Key words:

miR?496, DCUN1D1, cervical cancer