The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (20): 2580-2585.doi: 10.3969/j.issn.1006⁃5725.2021.20.003

• Basic Research • Previous Articles     Next Articles

Mechanism of inhibition of proliferation and invasion of glioma cells by miR ⁃ 106a ⁃ 5p targeting STAT3

ZHOU Hui*，LI Ruichun，YAN Dajun，CAI Dongpeng，WU Wenjiao，ZHONG Dequan，JIANG Xiaodan.   

  1. Neurosur⁃ gery Center，Zhujiang Hospital，Southern Medical University，the National Key Clinical Specialty，the Engineering Technology Research Center of Education Ministry of China on Diagnosis and Treatment of Cerebrovascular Disease， Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration，the Neurosurgery Institute of Guangdong Province，Guangzhou 510282，China；* Department of Neurosurgery，the First Affiliated Hospital of Guangdong Pharmaceutical University，Guangzhou 510080，China

  • Online:2021-10-25 Published:2021-10-25
  • Contact: JIANG Xiaodan E⁃mail：jiangxiao_dan@163.com

Abstract:

Objective To explore the mechanism of miR⁃106a⁃5p targeting signal transducer and activator of transcription 3（STAT3）in regulating the proliferation and invasion of glioma cells. Methods From January 2017 to December 2020，15 cases of tumor tissues and 5 cases of normal adjacent tissues（more than 1.5 cm away from the edge of the tumor）of patients with recurrent glioma who underwent surgical treatment were selected. Normal mouse neuron cells and glioma cell line GL261 were cultured in vitro. The expression of miR⁃106a⁃5p was detected by qPCR. An experimental group and a conctrol group were constructed. The experimental group included miRNA ⁃NC mimics，miR ⁃106a ⁃5p mimics，miR ⁃106a ⁃5p inhibitor plasmids were transfected with GL261 cells. Normally cultured GL261 cells were used as the control group.The expressions of miR⁃106a⁃5p and STAT3 in each group were detected by qPCR. Targeting relationship between miR⁃106a⁃5p and STAT3 was predicted and analyzed by the bioinformatics software and Dual luciferase assay. CCK⁃8 and Transwell chamber assay were used to detect cell proliferation activity and invasion ability of each group cells；The expressions of STAT3，apoptosis⁃related pro⁃ teins BAX，cl⁃cas⁃pase⁃3，and tumor suppressor gene p21 were detected by WB. Results The expression of miR⁃ 106a⁃5p in human recurrent glioma tissues and mouse glioma cells was significantly lower than that in paracancerous tissues and normal mouse nerve cells（P < 0.05）. Compared with that in the control group，the level of miR⁃106a⁃5p in GL261 transfected with miR⁃106a⁃5p mimics was significantly increased，while the expression of STAT3 was significantly decreased（P < 0.01）. Respectively，the expression in miR⁃106a⁃5p inhibitor group was opposite（P < 0.05）. STAT3 was the target gene of miR⁃106a⁃5p by dual luciferase assay. Compared with those in control group， the expression of STAT3，cell viability and number of invasive cells in the miR⁃106a⁃5p mimics group were signifi⁃ cantly decreased（P < 0.05），but the expression of BAX，cl ⁃caspase ⁃3 and p21 was significantly increased（P < 0.05）；However，the expression of these proteins，cell viability and number of invasive cells in the miR⁃106a⁃5p inhibitor group was the opposite correspondingly（P < 0.05）. Conclusion miR⁃106a⁃5p is low⁃expressed in human recurrent glioma tissues and GL261，and regulates the proliferation and invasion of glioma cells through negatively regulation of STAT3，which provides a new target for studying the recurrence mechanism of glioma.

Key words:

glioma, GL261 cells, miR?106a?5p, signal transducer and activator of transcription 3, proliferation, invasion