Abstract:

Objective To explore the expression of leptin and leptin receptor proteins in renal tubular cells in the negative energy state which induced by Caenorhabditiselegans muscle expression 3（Mex3c）. Methods FVB/N mice（n = 18）were selected and divided into three groups，i.e. the high⁃fat diet group（HFD group），the control group， and the Mex3c group. Observation of liver，fat and kidney tissues was conducted by pathological staining；LEPR and creatinine concentration in serum were determined by ELISA；the expression of leptin and LEPR in kidney tissues were determined by immunohistochemical staining，Western blotting and RT ⁃PCR. Results Pathological staining results：compared with the livers of the control group，there were lipid droplets in the Mex3c group and large lipid droplets in the HFD group. The abdominal fat cells in the HFD group were larger and fusion phenomena than those in the control group and Mex3c group. Compared with the control group，the gaps between the glomeruli and the cysts in the HFD group and the Mex3c group were larger. Compared with the leptin mRNA results of the control group，both the Mex3c group and the HFD group were highly expressed（P < 0.01）. Compared with the control group′ s LEPR mRNA results，the Mex3c group and the HFD group were all lowly expressed（P < 0.05）. The results of immunohisto⁃ chemistry showed that leptin and LEPR were expressed in the cell membrane of renal tubules. Western blot results showed that compared with the control group′s leptin and LEPR，the Mex3c group had high expression of leptin and low expression of LEPR（all P < 0.05）and the HFD group had high expression leptin and low expression of LEPR （all P < 0.01）. Compared with soluble LEPR of the control group ，the HFD group was highly expressed（P < 0.01）. Compared with the control group，the results of serum creatininein mice showed that the Mex3c group had low expres⁃ sion and the HFD group had high expression（all P < 0.01）. Conclusions The imbalance of positive and negative energy leads to high expression of leptin protein and low expression of LEPR protein in kidney tissue；in particular， imbalance of positive energy causes kidney function damage.

Key words:

 , Mex3c, HFD, leptin, leptin receptor, renal tubular cells, creatinine, high?fat diet