关键词: Sugen低氧模型, 肺动脉高压, 免疫细胞, 流式细胞术

Abstract:

Objective To investigate the dynamic changes of immune cell subsets in a mouse model of pulmonary hypertension （PH） induced by Sugen-hypoxia （SuHx）. Methods A SuHx-PH mouse model was established through intraperitoneal injection of Sugen 5416， followed by exposure to hypoxia. Mouse lung tissues were collected prior to modeling and at 1 week （h1w）， 2 weeks （h2w）， 3 weeks （h3w）， and 4 weeks （h4w） post-modeling. Flow cytometry was employed to detect the proportional alterations of immune cell subsets （including T cells， B cells， macrophages， eosinophils （EOS）， neutrophils （Neu）， natural killer （NK） cells， and innate lymphoid cells （ILCs） within CD45⁺ immune cells. Results Compared with the control group， the immune cell subsets in SuHx model mice demonstrated significant temporal changes. Among these， the total CD11c⁺F4/80⁺ macrophages and interstitial macrophages （IM， SIGLECF^-） showed a significant decreasing tendency as the modeling time progressed； the cell levels of alveolar macrophages （AM， SIGLECF⁺） continuously increased （P < 0.05）； EOS， ILCs， Neu， and CD4⁺T cells displayed a significant fluctuating pattern， with significant differences between groups （P < 0.05）. Conclusions The proportions of immune cell subsets in the lung tissues of SuHx-PH mice exhibit significant temporal changes， indicating that different immune cells may play stage-specific roles in the progression of PH. This study offers a foundation for a more in-depth understanding of the immunoinflammatory mechanism of PH and the identification of potential immune intervention targets.

Key words: Sugen-hypoxia model, pulmonary hypertension, immune cells, flow cytometry