关键词: 非小细胞肺癌, 放射治疗, 放疗敏感性, 长链非编码RNA

Abstract:

Objective To explore the effects of long non-coding RNA LINC01772 on cell cycle， apoptosis and radiotherapy sensitivity of A549. Methods A549 cells were resuscitated and cultured for experimental purpose. The effects of LINC01772 knockdown on cell viability， cell cycle progression， and apoptosis were assessed using CCK-8 assays and flow cytometry， and apoptosis-related proteins were analyzed using western blotting. CCK-8 and clonogenic assays were used to measure the changes in the sensitivity of A549 cells to radiotherapy following various radiation doses， and flow cytometry to examine alterations in the cell cycle and apoptosis post-radiation treatment. Results Following lentiviral transfection that reduced LINC01772 levels in A549 cells， a significant decrease in cell viability （P < 0.01） was observed alongside an increase in apoptosis （P < 0.01）. Notably， expressions of Bad and Cleaved Caspase-3 were significantly elevated （P < 0.01）， while Bcl-2 expression was markedly decreased （P < 0.01）. After exposure to different radiation doses， a dose-dependent reduction in A549 cell viability was noted （P < 0.01）， accompanied by a significant impairment of clonogenic ability （P < 0.01）， G2/M phase arrest within the cell cycle （P < 0.01）， and an increased incidence of apoptosis （P < 0.01）. Conclusion Inhibiting long non-coding RNA LINC01772 expression substantially diminishes cellular viability in A549 cells， enhances apoptotic processes， disrupts their cycling mechanisms， and augments their sensitivity to radiotherapy， suggesting that this non-coding RNA may serve as a potential target for enhancing lung cancer radiotherapy.

Key words: non-small cell lung cancer, radiation therapy, radiotherapy sensitivity, long non-coding RNAs