Patients with cardiovascular disease （CVD） are at high risk of coronavirus disease 2019（COVID⁃19）and the mortality of COVID⁃19 patients with CVD were significantly increased.Currently，there is noevidence that any antiviral drugs were effective to severe acute respiratory syndrome coronavirus 2（SARS⁃CoV⁃2）.In August，2020，National Health Commission issued Diagnosis and Treatment Protocol for COVID⁃19（Trial Version8）and the main antiviral drugs recommended for the treatment of COVID⁃19 are IFN⁃α，Ribavirin，Chloroquinephosphate，Arbidol，Lopinavir/ritonavir（LPV/r）and so on. All of these drugs have some adverse effects on cardio⁃vascular system. The review summarizes pharmaceutical care strategy for COVID⁃19 patients with cardiovasculardisease，and aims to provide useful guidance for pharmaceutical management and treatment of COVID⁃19 patientswith CVD.

Objective To explore the correlation between myocardial injury and inflammation in patientswith new coronavirus pneumonia（COVID⁃19）. Methods A retrospective study was conducted in 120 COVID⁃19patients who admitted to Wuhan First Hospital from January 20，2020 to March 20，2020. According to the clinicalclassification of COVID⁃19，these patients were divided into mild group（common type）and severe group（severeand critical type）. The clinical characteristics and Laboratory test，such as high⁃sensitivity troponin I（hs⁃TNI），creatine kinase⁃MB isoenzyme（CK⁃MB），lactate dehydrogenase（LDH），C⁃reactive protein（CRP），procalcitonin（PCT），white blood cell（WBC），neutrophil Cell（NEUT）and lymphocyte（LYM），were analyzed and comparedbetween the two groups. Furthermore，the difference and correlation were analyzed and compared. Results Firstly，among the three clinical types，the differences were statistically significant in age，gender，hypertension，coronaryheart disease and arrhythmia（all P < 0.05）. Secondly，the expressionof CK⁃MB，LDH and CRP and the proportionsof those abnormal indicators in the severe group were significantly increased，the same with the WBC and NEU，but opposite to the LYM（all P < 0.05）. Further more，in the severe group，the expressionof hs⁃TNI and PCT andthe proportions of those abnormal indicators in critical patients were significantly higher than those in severepatients（all P < 0.05）. Lastly，logistic regression analysis showed that the severity of COVID⁃19 was positivelycorrelated with the increase of hs⁃TNI，LDH，CRP，and PCT（P < 0.05）. Correlation analysis showed that hs⁃TNIwas positively correlated with CRP（r = 0.49，P < 0.001）and PCT（r = 0.318，P = 0.033），CK⁃MB was positivelycorrelated with CRP（r = 0.388，P = 0.002）and PCT（r = 0.264，P = 0.047），LDH was positively related to CRP（r = 0.349，P = 0.005）. Conclusions Among patients with Severe/critical COVID⁃19，old age and a history ofcardiovascular diseases are common，the severity of myocardial injury and inflammatory response are serious，andthe proportion is large. Moreover，the severity of myocardial injury is closely related to inflammatory response.

Objective To analyze the characteristics of peripheral blood lymphocyte subsets and cytokinesin patients with novel coronavirus（SARS⁃CoV⁃2）infection with normal CT at baseline，and to explore their rela⁃tionship to clinical outcome. Methods 48 patients of SARS⁃CoV⁃2 infection with normal CT at baseline admittedto Guangzhou eighth people′s Hospital from March to April 2020 were analyzed retrospectively. According to theirclinical outcome，the patients were divided into two groups：new diagnosis of pneumonia group and non⁃pneumoniagroup. Nonparametric rank sum test and Fisher exact probability method were used to analyze the differencebetween the two groups. Cox regression analysis was used to find out risk factors of new diagnosis of pneumonia.ROC curve analysis was used to predict the value of new diagnosis of pneumonia. Results 24 cases（15 males and9 females）were included in both new diagnosis of pneumonia group and non⁃pneumonia group. The average age ofnew diagnosis of pneumonia group and t non⁃pneumonia group was（38.25 ± 10.65）and（38.12 ± 10.89）years，respectively，and there was no significant difference between the two groups（P > 0.05）. Compared with non⁃pneu⁃monia group，the total number of T lymphocytes，CD4+ T lymphocytes，CD8+ T lymphocytes and B lymphocytes inperipheral blood of patients in new diagnosis of pneumonia group were lower and IL⁃6 were higher（Z = -2.588，-2.563，-2.746，-1.981 and -2.752，all P < 0 05）. But there was no significant difference in CD4+/CD8+ ratio，IL⁃2，IL⁃4，IL⁃10，TNF⁃α and IFN⁃γ pneumonia（OR = 5.500，95%CI：1.219～24.813，P = 0.027）. The area under ROC curve of IL⁃6 was 0.751，cutoffvalue was 3.39 pg/mL，sensitivity and specificity were 80.0% and 71.4%，positive predictive value and negative pre⁃dictive valuewere73.7%and78.1%，respectively.Conclusion In the patients with new diagnosis of coronavirus pneu⁃monia，T lymphocyte subsets and B lymphocytes are lower and IL⁃6 is higher. IL⁃6 has moderate predictive valuefor new diagnosis of coronavirus pneumonia. IL⁃6 may be the initiator of cytokine storm.

Renal cell carcinoma（RCC）is a common tumor of urinary system. The clinical prognosis ofadvanced RCC is poor. Targeted agents multi⁃target tyrosine kinase inhibitors（TKIs）and mammalian target ofrapamycin（mTOR）inhibitors are currently the main clinical treatment for advanced or metastatic RCC. Althoughprogression free survival（PFS）and overall survival（OS）of some patients are improved，treatment related adverseevents（TRAEs）and related drug resistance often lead to treatment interruption，which has limited the clinicalapplication of targeted agents. In recent years，the application of immune checkpoint inhibitors（ICIs）nivolumabin the treatment of patients with advanced RCC has achieved primary efficacy. We have reviewed the researchprogress of nivolumab in the treatment of advanced RCC.

Objective To study the effects of methyl methanesulfonate and UV sensitive gene clone 81（Mus81）silencing on proliferation and apoptosis of human hepatocellular carcinoma Hep3B cells. Methods Lenti⁃virus⁃mediated small interfering RNA（siRNA）was employed to inhibit Mus81 expression and real⁃time quantitativepolymerase chain reaction（Real⁃time PCR）was used to determine the inhibition efficiency of Mus81 expression.Growth curve was established by the methylthiazol tetrazolium（MTT）assay. The plate cloning experiment，propidiumiodide（PI）and Annexin V⁃APC staining were used to detect the proliferation，cell cycle distribution and apoptosisof Hep3B cell line. Results The efficiency of lentivirus infection was higher than 80%，and the efficiency of Mus81gene interference was 76.42%（P＜0.001）. The results of MTT growth curve and plate cloning experiment showedthat the growth rate of the Hep3B cell line silenced by Mus81 was decreased significantly（P < 0.001），the proliferationability was also significantly weakened（number of cell clones：（2 ± 1）vs.（54 ± 3），P < 0.001）. Annexin V⁃APCand PI staining showed that the apoptosis rate of Mus81 silenced Hep3B cell line was significantly increased［（20.87 ± 0.82）% to（0.69 ± 0.09）%，P < 0.001］and the proportion of cells in G1 phase was slightly decreased［（33.77 ± 1.61）% to（42.06 ± 0.40）%，P < 0.05］. Conclusion Mus81 gene silencing can significantly inhibit thegrowth and proliferation of human liver cancer Hep3B cells and promote its apoptosis.

Objective To observe the combinative effects of paeoniflorin and temozolomide on the prolifera⁃tion and migration of primary human glioma cells in vitro，and preliminary explore its mechanism of action. Methods The MTS method was used to detect the effects of paeoniflorin，temozolomide and the combination of the two drugson the viability of primary human glioma cells. The equivalent line diagram was used to analyze the synergisticeffect. EdU staining was used to detect cell proliferation. Transwell cell migration experiment was used to detect cellmigration. The reverse pharmacophore docking technique was used to explore the potential target of paeoniflorin toinhibit glioma cells and Western blot to verify. Results Paeoniflorin and temozolomide inhibited primary humanglioma cells viability in a concentration⁃ and time⁃dependent manner. The combination of the two drugs had a syner⁃gistic inhibitory effect；the inhibitory effect of the combination of the two drugs on cell proliferation and migration wassignificantly higher than that of the paeoniflorin and temozolomide monotherapy group. The effect group dockingtechnique suggests that Src may be the target of paeoniflorin. Western blot analysis showed that paeoniflorin cansignificantly inhibit the expression of p⁃Src and p⁃STAT3. Inhibition of migration⁃related proteins. Conclusion Paeoniflorin can significantly improve the anti ⁃ glioma effect of temozolomide. This synergistic effect is mainlythrough inhibiting the activation of Src/STAT3 signaling pathway，thereby further enhancing the inhibitory effect onproliferation and migration⁃related proteins and inhibiting the proliferation and migration of glioma cells.

Objective To study the expression of long non⁃coding RNA（lncRNA）ZNF571⁃AS1 inprostate cancer，to clarify its effect on the proliferation and invasion of prostate cancer cells and its molecularmechanism. Methods Real⁃time quantitative polymerase chain reaction（qPCR）was used to detect the expres⁃sion of ZNF571⁃AS1 in prostate cancer tissues，adjacent tissues，prostate cancer cell lines and normal prostateepithelial cell. The cell line with the most expression of ZNF571⁃AS1 was used as a study. The plasmid thatsilenced the ZNF571⁃AS1 gene and the negative control plasmid were transfected separately. The effects of ZNF571⁃AS1 on the proliferation and invasion of prostate cancer cells were detected by cell proliferation experiment（MTTmethod）and Transwell experiment. Bioinformatics technology predicts the molecular mechanism of ZNF571⁃AS1.qPCR and Western blot methods were used to detect the expression of downstream target genes. Results Theexpression of ZNF571⁃AS1 in prostate cancer tissue was significantly higher than that in adjacent tissues（P < 0.01）.The expression of ZNF571⁃AS1 in prostate cancer cells was significantly higher than that in normal prostate epithelialcells（P < 0.01），and the expression in DU⁃145 cells was the most（P < 0.01）. Low expression of ZNF571⁃AS1can significantly inhibit the proliferation and invasion ability of DU⁃145 cells（P < 0.05）. The downstream targetgene of ZNF571⁃AS1 may be miR⁃301b⁃3p，and the target gene of miR⁃301b⁃3p may be androgen receptor（AR）.Low expression of ZNF571⁃AS1 can significantly up⁃regulate the expression of miR⁃301b⁃3p in DU⁃145 cells（P < 0.01），and inhibit the expression of AR gene（P < 0.01）. Conclusion ZNF571⁃AS1 is significantly up⁃regulated in prostate cancer tissues and cell lines. ZNF571⁃AS1 may affect the proliferation and invasion of prostatecancer DU⁃145 cells by regulating the miR⁃301b⁃3p/AR signaling pathway.

Objective To investigate the effect of microRNA（mi）⁃365 on autophagy in hepatocellularcarcinoma（HCC）cells by regulating autophagy related gene 3（ATG3）. Methods Collected the cancerous tissuesand adjacent tissues of HCC patients，purchased human liver cancer SMMC⁃7721 cells，liver cancer HepG2 cells，human normal liver cells L02，transfected SMMC⁃7721 cells，and randomly divide them into control group and NCgroup（transfected with miR⁃365⁃NC）and overexpression group（transfected with miR⁃365 mimics）. RT⁃qPCRwas used to detect the expression levels of miR⁃365 and ATG3 in different tissues，different liver cancer cell linesand normal hepatocytes，MTT method was used to detect the proliferation of SMMC⁃7721 cells after transfection，flow cytometry was used to detect cell apoptosis，transmission electron microscopy and MDC staining was used toobserve cell autophagy，Western blot was used to observe the relative expression of ATG3，microtubule⁃associatedprotein1 light chain3⁃II（LC3⁃II），p62，Beclin1 protein in cells，dual luciferase experiment was used to detect thetargeting of miR⁃365 and ATG3. Results The expressions of miR⁃365 and ATG3 in cancer tissues of HCC patientswere lower than those in adjacent tissues（P < 0.05）. The expressions of miR⁃365 and ATG3 in liver cancer SMMC⁃7721 cells and HepG2 cells were lower than those of normal liver cells L02（P < 0.05）. After transfection of miR⁃365，compared with the control group and NC group，the expression of miR⁃365 and ATG3 in the overexpressiongroup increased，cell proliferation decreased，and the apoptosis rate increased（P < 0.05）. In the overexpressiongroup，autophagic vesicles were formed and the fluorescence intensity of MDC staining increased. Compared withthe control group and the NC group，the overexpression group ATG3，LC3⁃II，Beclin1 protein expression increased，and the p62 protein expression decreased（P < 0.05）. The dual luciferase experiment showed that ATG3 is thetarget gene of miR⁃365. Conclusions Overexpression of miR⁃365 can up⁃regulate the expression of ATG3，in⁃duce autophagy，inhibit the proliferation of SMMC⁃7721 cells，and promote cell apoptosis.

Objective To investigate the mechanism of activated protein C to alleviate the mitochondrialenergy metabolism disorder induced by Mycobacterium tuberculosis in human airway epithelial cells by targetingVLA⁃3⁃neutrophil subpopulation. Methods Human bronchial epithelial BEAS⁃2B cells were divided into threegroups：BEAS⁃2B group（normal culture cell line），Mycobacterium tuberculosis induction group（BEAS⁃2B cellculture was infected with 50 bacteria/cell conjugated bacteria），and BEAS⁃2B transfection group（BEAS⁃2B cellswere transfected with lentiviral vector carrying activated protein C）. The binding degree of activated protein C to vla⁃3 was determined by soluble integrin binding. RT qPCR was used to analyze the mRNA expression of IL⁃6，IL⁃8and MCP⁃1. The function of mitochondria was evaluated by fluorescence probe JC⁃1 and mitochondrial membranepotential was measured by biosciences. Cell apoptosis and viability were detected by ELISA and TUNEL. Results VLA⁃3 was more highly expressed than VLA⁃3 and αVβ3（P < 0.05）. The expression of IL⁃6，IL⁃8 and MCP⁃1mRNA in the induction group of Mycobacterium tuberculosis was higher than that in BEAS⁃2B group（P < 0.05）. TheBEAS⁃2B transfection group was more sensitive than IL⁃6，IL⁃8 and MCP in the induction group of Mycobacteriumtuberculosis. 1 mRNA expression was decreased（P < 0.05）. Compared with the Mycobacterium tuberculosis induc⁃tion group，the mitochondrial complex Ⅰ and mitochondrial complex Ⅳ activity were significantly increased in theBEAS⁃B group（P < 0.05），and the mitochondrial complex I and mitochondrial complex Ⅳ activity were decreasedin the Mycobacterium tuberculosis induction group compared with the BEAS⁃B transfection group（P < 0.05）. Themembrane potential of the BEAS⁃2B group was higher than that of the Mycobacterium tuberculosis induced group（P < 0.05），and the membrane potential of the tuberculosis induction group was lower than that of the BEAS⁃2Btransfection group（P < 0.05）. The apoptosis rate of BEAS⁃2B group was lower than that of Mycobacterium tubercu⁃losis induction group（P < 0.05），and the apoptosis rate of Mycobacterium tuberculosis induction group was higherthan that of BEAS⁃2B transfection group（P < 0.05）. The cell viability of the BEAS⁃2B group was higher than thatof the Mycobacterium tuberculosis induction group（P < 0.05），and the cell viability of the tuberculosis inductiongroup was lower than that of the BEAS⁃2B transfection group（P < 0.05）. Conclusion Activated protein C has astronger affinity for integrin VLA3，which can target VLA3 neutrophil subpopulation to reduce the inflammatoryresponse of human airway epithelial cells，improve mitochondrial complex Ⅰ，complex Ⅳ activity，and effectivelyinhibit binding. Bacillus⁃induced decrease in mitochondrial membrane potential.

Objective Retrospective observation of hemodialysis（HD）and peritoneal dialysis（PD）inpatients ESRD with HFpEF compared in dialysis adequacy，electrolytes，nutritional status，the number of re⁃hospi⁃talization due to heart failure and the improvement of New York Heart Association grade at the end of follow⁃up.Methods Clinical data of patients treated with HD and PD in Zhujiang Hospital of Southern Medical Universityfrom January 2010 to June 2019 were collected. The patients with dialysis duration less than 3 months were excluded.The dialysis adequacy，electrolytes，nutritional status，times of re⁃hospitalization due to heart failure and the im⁃provement of New York Heart Association grade were compared between the two groups before the first dialysis andat the end point of follow⁃up. Results There was no significant difference in age，sex，BMI，MAP，heart rate，blood biochemistry and kidney function，NT⁃proBNP，New York Heart association grade and medication betweentwo groups（P > 0.05）. At the end of follow⁃up，the NYHA grade and the number of rehospitalization due to heartfailure in the HD group were significantly lower than those in the CAPD group（P < 0.05）. The levels of serumcreatinine，urea nitrogen and uric acid were decreased significantly in both groups（P＜0.01），but the levels ofserum creatinine and urea nitrogen in HD group were much significantly lower than those in PD group（P < 0.01）.The levels of serum albumin and serum potassium in HD group were significantly higher than those in PD group atthe end of follow⁃up. There was no significant difference in mean arterial pressure and blood lipid between the twogroups（P＞0.05）. Conclusion In this study group，We conclud that the improvement of cardiac function，dialysisadequacy，electrolyte disturbance and nutritional status in the HD group are superior to those in the PD group atthe end of follow⁃up

Objective To investigate the clinical significance of anorectal dynamic examination inpatients with constipation by studying the changes of anorectal dynamic indexes and their correlation with clinicalsymptoms. Methods Retrospective analysis of 187 patients diagnosed with constipation，including 3 D high reso⁃lution anorectal pressure measurement obtained by the Anorectal pressure gradient，Relaxation rate of sphincter，Balloon extraction time and Knowles⁃Eccersley⁃Scott（KESS）constipation symptoms score，the Anorectal pressuregradient，Relaxation rate of sphincter，Balloon extraction time and constipation symptoms score were compared andanalyzed. Results The relaxation rate of sphincter and the average relaxation rate were less than 20% respectively，Anorectal pressure gradient was negative，There was no significant correlation between the relaxation rate of sphinc⁃ter and the time of balloon extraction（P > 0.05），There was a negative correlation between Anorectal pressuregradient and total clinical symptoms（Correlation coefficient P = 0.006，rs = -0.212，Regression coefficient F =4.229，P = 0.041）. Conclusion The relaxation rate of sphincter，balloon extraction time and the severity of clini⁃cal symptoms are not significantly correlated.Anorectal pressure gradient and clinical symptoms show a negativecorrelation. If the Anorectal pressure gradient is greater，the KESS score is lower，the clinical symptoms is lighter，which means the greater the abdominal pressure，the smaller the anal residual pressure，the lighter the constipa⁃tion symptoms.

Objective To explore the epidemiological characteristics and prognostic factors of fever andthrombocytopenia syndrome（SFTS）caused by new bunya virus（SFTSV），and provide clinical basis for earlyidentification，diagnosis and treatment. Methods The case data of 80 SFTS patients admitted to the Departmentof Infectious Diseases，Anhui Medical University First Hospital from January 2019 to June 2020 were collected anddivided into survival group（n = 59）and death group（n = 21）. The two groups were compared in terms of generalinformation，symptoms and signs，laboratory indicators and viral load. The correlation between laboratory testresults and prognosis was analyzed by Spearman correlation analysis and prognostic related factors were analyzedwith multivariate binary logistic regression. Results The length of stay in the survival group was statistically signif⁃icantly longer than that of the death group（P < 0.05）. The levels of eGFR1，Ca，PLT，CD4，and CD8 in the survivalgroup were significantly higher than those in the death group（P < 0.05）. The levels of K，ALT，AST，ALP，GGT，LDH，CRE，CK，CKMB，fasting blood glucose，CRP，PCT，I1B，I6，I8，IL⁃10，IL⁃2R，TNA，APTT，TT，D⁃D，FDP and the viral load of Bunia virus in the survival group were all significantly lower than the deathgroup（P < 0.05）. Conclusion Nervous system manifestations（positive meningeal stimulation sign or disturbanceof consciousness at different degree），APTT level and the logarithm of viral load may be the factors influencing theprognosis of the disease.

Objective To investigate the different effects of partial plasma exchange combined with dualplasma molecular adsorption and pure plasma exchange on short⁃term survival rate of patients with acute⁃on⁃chronicliver failure（ACLF）. Methods Data of 53 hospitalized patients with ACLF who received artificial liver therapyincluding 30 treated with dual plasma molecular adsorption combined with partial plasma exchange and 23 withsimple plasma exchange，were retrospectively analyzed. Related serum biochemical indexes and survival rates at the4th and 12th week were compared between the two groups before and after the treatment，and clinical prognosticfactors of ACLF patients were analyzed by univariate analysis. Results Liver function were improved in both groupsafter the treatment，and the difference was statistically significant（P < 0.05）. Cumulative survival rate of the twogroups at the 4th week was 86.96% and 96.67% respectively and the follow⁃up cumulative survival rate at the 12thweek was 86.67% and 69.56% respectively，showing no statistical significance（P > 0.05）. Conclusion Dual plasmamolecular adsorption combined with partial plasma exchange and pure plasma exchange effectively improve therelevant biochemical indexes of patients with ACLF，but they play no significant role in short⁃term survival rate.

Objective To investigate the correlation of procalcitonin（PCT）and creactive protein（CRP）with sepsis⁃related organ failure assessment（SOFA）and quick sepsis⁃related organ failure assessment（qSOFA）score in sepsis patients as well as the risk factors that affect the prognosis of sepsis patients. Methods The clinicaldata of the patients admitted to the intensive care unit（ICU）of the People′s Hospital of Wudang and The SecondPeople′s Hospital of Guiyang，from January 2016 to June 2020 were retrospectively analyzed. 106 sepsis patientsunderwent PCT and CRP testing，and SOFA and qSOFA scores were evaluated. The patients were divided intosurvival group（n = 82）and death group（n = 24）according to the prognosis. The PCT，CRP，SOFA and qSOFAscore of the two groups were compared. The PCT and CRP values of different SOFA evaluation groups and qSOFAevaluation groups were compared. Spearman correlation analysis was used to analyze the correlationship of PCT andCRP with SOFA and qSOFA. The influencing factors of sepsis were analyzed by regression. Results Comparedwith the survival group，the incidence of drinking and hypertension was higher in the death group. PCT，SOFA andqSOFA scores were significantly increased（P < 0.05）. PCT increased significantly with the increase of SOFA andQ⁃SOFA scores（P < 0.05）. PCT was positively correlated with SOFA and qSOFA scores（r > 0，P < 0.05）. CRPwas not associated with SOFA and qSOFA scores（P > 0.05）. PCT，SOFA and qSOFA scores were risk factors for death in sepsis patients（P < 0.05）. Conclusion Compared with CRP，PCT can better reflect the severity andprognosis of sepsis patients. When PCT is used in combination with SOFA and qSOFA score，it is more beneficialfor clinicians to judge and evaluate the condition.

Objective To study and establish a multiple linear regression model predicting neonatal weightof non⁃obesity and non⁃diabetic maternal. Methods A total of 86 pregnancy maternal were admitted to this hospitalfrom December 2017 to December 2018 were selected as research objects，and they were measured by variousindicator. Several indicators were studied between two groups divided by different average neonatal weight. Then wecan analyze relationship between serious of indicators and neonatal weight by Pearson correlation analysis. A multiplelinear regression model was established which can predict neonatal weight by clinic several indicators and ultrasonicindicators. Then we compared prediction capacity of the mode with other methods. Results There was statisticallysignificant difference（P < 0.05）that weight gains in pregnancy，gestational weeks，uterine height and maternalabdominal circumference，fetal circumference，biparietal diameter and femur length，which were increased alongwith birth. The correlation coefficients of the above indicator on neonatal weight is 0.288，0.366，0.496，0.380，0.419and 0.522，respectively（P < 0.05）. The optimal linear regression model predicted the neonatal weight（F = 22.7，P < 0.005）was 111.2 X1 + 44.5 X2 + 336.1 X3 4454.7（X1，X2，X3 is respectively fetal circumference，fundal height，fetal femur length），which could predict 54% of the neonatal weight.This model of the coincidence rate was signifi⁃cantly higher than other methods（P < 0.05），with higher accuracy for predicting neonatal weight. Conclusion Theprediction regression model is able to predict neonatal weight and the formula is better predictor than other method.

Objective To invest the quality of life（QOL）of elderly patients with type 2 diabetes mellitus（T2DM）and coronary arteries atherosclerosis（CAAS），analyze the major influencing factors and put forward nurs⁃ing countermeasures. Methods To gather clinical records of our 120 hospitalized elderly patients with T2DM andCAAS and conduct survey with general questionnaire，diabetes⁃specific quality of life（DSQL）and summary ofdiabetes self⁃care activities（SDSCA）. QOL⁃related factors are filtered with T test，variance test and correlationanalysis，and factors influencing patients′ QOL are analyzed with multiple linear regression equation model. Drawthe receiver operating characteristic（ROC）diagram to select indicator for predicting QOL. Results The DSQLscore of the 120 included subjects were（70.97 ± 21.41），among which the QOL of psychological dimension is theworst；and the SDSCA score was（36.92 ± 12.39）with blood glucose monitoring capability the worst. According tostatistical analysis，age，education level，course of diabetes，blood sugar control level，family history of diabetesand self⁃care activities are related factors of QOL. Age，education，course of disease，blood sugar control and self⁃careactivities are the main factors that affect the QOL of the subjects（P＜0.05）. The regression equation modelshows that the older patients with the longer course of disease，worse blood sugar control and weaker self⁃careactivities，received worse QOL score. Area under ROC curve was 0.698（95%CI：0.604 ~ 0.793，P＜0.001），self⁃care activities were an important index to predict QOL. Conclusion The QOL of elderly patients with T2DM andCAAS is generally poor. It is necessary to enhance the self⁃care activities of the patients，especially blood glucosemonitoring care；actively improve the mental and psychological health of the patients；pay attention to the influ⁃ence of risk factors such as age and disease course to improve the QOL of patients.

Objective To investigatethe level of vitamin D in cord Blood with PROM and its relationshipwith early onset neonatal sepsis. Methods 80 cases of full⁃term neonates with PROM who were delivered in thematernity ward，the First Hospital of Hebei Medical University were selected from January 2018 to December 2018.They were divided into the early onset sepsis（EOS）group of 24 cases and the non EOS group of 56 cases. 30 healthyfull⁃term neonates born in the same period were selected as the control group. The cord blood of vitamin D levelswere measured with high performance liquid chromatography tandem mass spectrometry. The cord blood of PCTlevels were measured with micromagnetic grain of chemiluminescence immunoassay. Results The levels of cordblood 25（OH）D in non⁃EOS and EOS group were statistically significant compared with those in the control group（P < 0.05，P < 0.01）. The 25（OH）D levels of cord blood in the EOS group were statistically significant comparedwith the non⁃EOS group（P < 0.05）. Cord blood 25（OH）D was negatively correlated with PCT in EOS group（r = -0.511，P < 0.05）. The cord blood 25（OH）D deficiency rate was 91.7% in the EOS group ，73.2% in the nonEOS group ，40% in the control group，and statistically significant in the three groups（χ2 = 17.738，P < 0.01）. EOSgroup and non EOS group were statistically significant compared with the control group（all P < 0.01）. Conclusions The low level of cord blood 25（OH）D has a correlation with early onset neonatal sepsis.

Objective To investigate the anticoagulation effect and safety of heparin⁃free，one⁃stage andtwo⁃stage citrate anticoagulation during hemodialysis with calcium⁃containing dialysate. Methods Hemodialysispatients with high risk of bleeding were randomly divided into three groups：heparin⁃free group（N⁃HEP group），one⁃stage anticoagulant group（S⁃RCA group），and two⁃stage anticoagulant citrate group（TS⁃RCA group），with atotal of 66 cases to complete the hemodialysis. The N⁃HEP groups were treated with normal saline flush pipeline，the S⁃RCA group with 4% citrate anticoagulants pumped in the front of the dialyzer and the TS⁃RCA group with 4%citrate anticoagulants pumped in the front of the dialyzer and at the venous pot as well. The three groups werecompared in terms of the hemodialysis treatment time，anticoagulant efficiency and safety. Results The treatmenttime of the S⁃RCA group and the TS⁃RCA group was both significantly longer than that of the N⁃HEP group（P＜0.05）. There was no significant difference in anticoagulant efficiency among the three groups at the hemodialysisfilter（P > 0.05）. The anticoagulation efficiency of the S⁃RCA group and the TS⁃RCA group was significantly higherthan that of the heparin⁃free group（P < 0.05），while the difference between the S⁃RCA group and TS⁃RCA groupwas not statistically significant（P > 0.05）. After hemodialysis，serum free calcium in both the S⁃RCA group andTS⁃RCA group significantly decreased compared with that before hemodialysis（P < 0.05）. Conclusions Forhemodialysis patients with high risk of bleeding，anticoagulation with citrate is superior to anticoagulation withoutheparin. It is safe and effective.

Objective This study focused the safety and efficacy of popliteal artery access for retrograderecanalization of superficial femoral artery（SFA）chronic total occlusion（CTO）lesions. Methods The clinicaland one year follow⁃up data of patients with SFA CTO lesions underwent endovascular recanalization in The First Affiliated Hospital of Zhengzhou University from March 2012 to March 2019 were reviewed retrospectively. The antegrade recanalization of SFA CTO through conventional contralateral femoral artery access in the supine positionwas selected as FA group（2 265 patients）. The retrograde recanalization of SFA CTO through popliteal arteryaccess in the prone position was PA group（216 patients）. Results The two groups had similar outcome of technicalsuccess rate，usage rate of stent and peri⁃operative（30 days）adverse event，which included death，arterial dissec⁃tion，distal embolization，puncture complications. FA group had obviously higher rate of CTO equipment usage andmore longer procedure time and fluoroscopy time than PA group（P＜0.05）. There were no difference between twogroups in 1⁃year primary patency，amputation⁃free survival，incidence of major adverse cardiovascular event，which consisted of myocardial infarction，stroke and death from any cause. Conclusions The recanalization ofSFA CTO lesions through PA access was safe and effective comparable with conventional FA access. This techniqueshould be considered as alternative with FA access.

Objective To quantitatively analyze the fibrosis characteristics of hypertrophic cardiomyopathy（HCM）by T1 mapping technique，and analyze the basic data of HCM patients. Methods Twenty⁃eightcases withHCM patients and 16 cases（normal group）were examined with T1 mapping sequence，delayed enhanced scan andlaboratory examination. The initial T1 values of hypertrophic and non⁃hypertrophic myocardium in case group，normal group and case group were statistically analyzed.According to delayed enhanced scan，patients were dividedinto negative group and positive group. The differences of myocardial T1 and basic data between LGE negative，LGE positive and normal groups were compared. The ROC curve was constructed to analyze the diagnostic value ofT1 value in distinguishing LGE negative from normal group，LGE positive from normal group. Results The nativeT1 value in the HCM group were larger than those in the normal group. The initial T1 values of hypertrophic aerawere larger than those in non⁃hypertrophic myocardium. The difference was statistically significant（P < 0.001）.There were significant differences among LGE negative，LGE positive and normal groups（P < 0.001）. There wasno significant difference in ejection fraction and NT⁃proBNPamong the two groups，but the maximum short diameterof LGE negative group was lower than that of LGE positive group，and the maximum long diameter was opposite.The sensitivity and accuracy of T1 value in distinguishing LGE negative from normal group were more than 85%，respectively，and the accuracy were 93.1% between LGE positive and normal groups. Conclusion T1 mappingtechnique can quantify the degree of myocardial fibrosis. The value of T1 is related to the degree of myocardial hyper⁃trophy.

Objective To differentiate minimally invasive adenocarcinoma（MIA）from invasive adenocar⁃cinoma（IAC）presenting as pure ground⁃glass nodules（pGGNs）using CT imaging features and a neural networkmodel. Methods We retrospectively collected 151 patients with single MIA and 127 patients with single IACpresenting as pGGN on thin⁃slice CT surgically and pathologically confirmed in our hospital from January 2015 toJanuary 2018. The clinical and imaging features of all patients were collected and the differences of these featureswere compared between MIA group and IAC group. The input variables and factors based on p value less than 0.05with univariable analysis were used to establish a neural network model. The subject operating characteristic（ROC）curve was used to analyze，calculate and compare for predicting the diagnostic efficiency of IAC. Results Clinically，the average age of patients in MIA group was lower than that in IAC group（P < 0.001）. In imagingmorphology，the incidence of circular in MIA group was higher，but the incidences of lobulated sign，vesselconvergence sign，air bronchogram，cavitation and pleural indentation sign were less than in IAC group（all P <0.001）. In imaging quantitative analysis，mCT⁃LP，mCT⁃W，diameter，volume and mass were significantly lowerin MIA group than in IAC group（all P < 0.001）. The ROC curve of the neural network model was significantlybetter than the quantitative variables，with an AUC of 0.91，accuracy，sensitivity and specificity of 81.00% ，78.81% and 86.61%，respectively. Conclusions It is helpful to differentiate MIA from IAC presenting as pGGNsusing the neural network model.

Objective To compare the diagnostic value of Color Doppler Ultrasound and MRI combinedwith myocardial injury markers in left ventricular aneurysm（LVA）in myocardial infarction. Methods The Colordoppler Ultrasound andMRI data of 30 suspected LVApatients from Jan 2019 to Jan 2020 were collected，the diag⁃nostic value of LVA was compared with pathological results according to the results of myocardial injury markers.Results The positive rate of LVA and LVA distribution by Color Doppler Ultrasound and MRI was100.00%，thedifference was not statistically significant（P > 0.05），the detection rate of Color Doppler Ultrasound and MRIcontradictory movement area was not significantly different（P > 0.05）；there was no significant difference in theaccuracy of Color Doppler Ultrasound and MRI diagnosis of true LVA（P > 0.05），and there was no significantdifference between CK⁃MB and cTnIt in the diagnosis of true LVA and false LVA（P > 0.05）；The value of D⁃dimer（D⁃D）in true LVA were higher than those in false LVA（P < 0.05）. Conclusion Both Color Doppler Ultrasoun⁃dand MRI have diagnostic value in diagnosis of LVA，Color Doppler Ultrasoundcan be used as the first choice fordiagnosis，MRI can be used as a supplementary examination method；D⁃D is beneficial to the differential dignosisof true LVA and false LVA.

Objective To protect contraceptive safety，analyze the adverse events of commonly used intra⁃uterine devices（IUD）. Methods IUD adverse event reports were collected from 2011 to 2019 and populationinformation of using IUD database were linked to calculate the incidence rate of 10 common IUD adverse eventsand compare the risk. Results In the first year after IUD placement，the average incidence of adverse events of10 common IUD was 2.66%. With the prolongation of use time，the incidence of adverse events of IUD（exceptactive γ type）decreased gradually. However，the incidence of active γ type increased gradually. The cumulativeincidence of IUD in 3 and 5 years was 11.70% and 19.96% respectively，which was significantly higher thanthe average incidence. The relative risk（RR）was 1.69（95%CI：1.55 ~ 1.83）and 1.84（95%CI：1.72 ~ 1.98），respectively. The cumulative incidence of adverse events in 3 and 5 years of MLCu375 ranked the second place，8.30% and 12.33% respectively，and the incidence was also higher than the average incidence，RR was 1.20（95%CI：1.12 ~ 1.28）and 1.14（95%CI：1.07 ~ 1.21），respectively. Conclusion It is suggested that we should furtherstrengthen the monitoring of adverse events of contraceptives，improve the quantity and quality of reporting，andreduce the underreporting. The number of IUD varieties with high risk of adverse events shall be appropriatelyreduced. Regular follow⁃up and timely treatment shall be carried out after the IUD is placed，and the use of IUDwithin two years after the device is placed and near the placing period shall be focused，so as to reduce thewithdrawal of the cause of disease and promote the contraceptive safety.

Objective To investigate the incidence of anxiety and depression in PCOS patients in Guang⁃dong Women and Children′s Hospital and analyze the risk factors of the disease so as to provide guidance forclinical treatment. Methods PCOS patients who were admitted to the Guangdong Women and Children Hospitalfrom August 2017 to February 2019 were selected as subjects for the survey using GAD⁃7，PHQ⁃9 Symptom Check⁃list and Sleep Questionnaire. The patient′s mental state and sleep quality were assessed，and the patient′s generalclinical data and blood biochemical indicators were collected for analysis. Results Of the 262 patients enrolled，80（30.5%）had anxiety disorder and 76（29.0%）had depressive disorder. Sleep disorders（OR = 3.544，95%CI：1.641 ~ 7.652）was an independent risk factor for the depression in patients with PCOS. Sleep disorders（OR =3.938，95%CI：1.804 ~ 8.597）and WHR（OR = 94.904，95%CI：1.328 ~ 6 780.675）were independent risk factorsfor anxiety in patients with PCOS. Conclusion Patients with PCOS are prone to comorbid anxiety and depression.Sleep disorders is an independent risk factor for the onset of depression and anxiety in patients with PCOS. Waist⁃to⁃hip ratio is an independent risk factor for the onset of anxiety in patients with PCOS. Clinicians should pay attentionto the screening of anxiety and depression among PCOS patients.

Objective A new method of 3⁃dimensional conformal planning design is proposed and itsdosimetric advantages in stereotactic body radiotherapy（SBRT）are evaluated. Methods A retrospective studywas conducted in 30 patients treated with SBRT technology. The conventional 3⁃dimensional conformal planning（CCP） and the deformed target 3⁃dimensional conformal planning （DCP） were designed respectively. Theprescription dose of each plan is defined as the planning target volume（PTV）50 Gy/5 fractions，and the PTVcoverage was required to be ＞95% for prescription dose. The plans were compared by assessing the conformityindex（CI），dose gradient（DG），approximate minimum dose of PTV（D99%），maximum dose at 2 cm from PTV inany direction（D2 cm）and monitor unit（MU）. Results Both groups of plans satisfied all clinical requirements.CI，DG，D99%，and D2 cm in the group DCP were significantly improved（t = 4.55，5.95，4.37，3.86，P < 0.05）andMU decreased（t = 2.82，P < 0.05）compared with the group CCP. Conclusions DCP has obvious dosimetricadvantages，and reduces the number of MU while shortening the treatment time. It can be promoted as a new planningdesign method.

Objective To investigate the effect of Sanjiao Regular Sequence Therapy on chronic heartfailure and its effect on neuroendocrine hormone activity. Methods In January 2019 ⁃ January 2020 at the recordsof 200 patients with CHF，multicenter randomized allocation scheme were divided into control group（n = 100 cases，with regular treatment）and treatment group（n = 100 cases，Sanjiao Regular Sequence Therapy based on the controlgroup），LHFQ score，6 min walking distance test（6 MWT），left ventricular ejection fraction（LVEF），brainnatriuretic peptide precursor（NT⁃proBNP），angiotensin Ⅱ（AngⅡ）level before treatment and after treatmentwere compared between two groups，and the curative effect of TCM syndrome integral of the two groups wereassessed. Results The effective rate was 82.00% in the treatment group and 68.00% in the control group，and thedifference between the groups was statistically significant（Z = 2.439，P < 0.05）. After treatment，the LHFQscore，nt⁃probnp and ang⁃ii in the two groups decreased（P < 0.05），and the LHFQ score，NT⁃proBNP and ang⁃iiin the treatment group were lower than those in the control group（P < 0.05），and the LVEF in the 6 min walkingdistance and LVEF in the treatment group were higher than those in the control group（P < 0.05）. Conclusion The treatment of chronic heart failure with three⁃focal therapy has a significant effect ，which can significantlyimprove the quality of life，increase the exercise tolerance，reduce the activities of NT⁃proBNP，Ang⁃Ⅱ and otherneuroendocrine hormones，inhibit ventricular remodeling，and promote the improvement of cardiac function.

Sepsis is defined as life⁃threatening organ dysfunction caused by a dysregulated host response toinfection. With complicated pathogenesis and high mortality，it is intractable to treat. Continuous renal replacementtherapy（CRRT）is an umbrella term of long⁃time continuous extracorporeal blood purification which was practicedfor replacing injured kidney function but CRRT has been set for bringing critically ill patients out of danger. Theeffect on improving survival rate of CRRT treating sepsis is embodied in clearing inflammatory mediators andcytokines，improving hemodynamic disorder and protecting organ function. This review focuses on the advantagesand disadvantages of various CRRT treatment modes，related blood filters and membrane materials in the treatmentof sepsis.