Objective To explore the efficiency of nomogram based on quantitative CT in prediction of severe corona virus disease 2019（COVID⁃19）. Methods The clinical and chest CT data on 117 COVID⁃19 patients were retrospectively analyzed. All the patients were divided into a mild group（82 patients）and a severe group （35 patients）. Multivariate Logistic regression analysis was performed on the clinical and quantitative CT indexes which had significant differences between the two groups to determine the independent risk factors associated with severe COVID⁃19. Nomogram was constructed for predicting severe COVID⁃19，and then verified by ROC analysis， calibration curve and Hosmer⁃Lemeshow goodness⁃of⁃fit test. Results Multivariate logistic regression showed that age，lymphocyte count⁃to⁃white blood cell ratio，LeV%，and MLeD were independent risk factors for severe COVID⁃ 19. The area under the curve of nomogram was 0.969. The calibration curve showed that the predicted probability was highly concordant with the actual probability. Hosmer⁃Lemeshow goodness⁃of⁃fit test（χ2 = 4.352，P = 0.824） showed that nomogram had higher efficiency in predicting severe COVID⁃19. Conclusions The nomogram based on quantitative CT has better efficiency and application value in predicting severe COVID⁃19. 

Objective To explore the relationship between viral load and clinical characteristics by analyz⁃ ing the early nasal swab viral load of Delta variant of New Coronavirus（SARS ⁃Cov ⁃2）. Methods One hundred and eight patients infected with Delta variant of SARS ⁃ Cov ⁃ 2 treated in Guangzhou Eighth People′ s Hospital， Guangzhou Medical University from May 21 to June 18，2021 were selected. The levels of N gene and Orf1a/b gene of nasal swab virus RNA and the viral nucleic acid of nasal swab were detected by real⁃time polymerase chain reac⁃ tion（RT⁃PCR）. According to the cycle threshold（Ct）value，the patients were divided into high copy group and low copy group，and the clinical characteristics and viral nucleic negative conversion time were compared between the two groups. Results Fifty⁃nine cases were included in high copy group，while forty⁃nine in low copy group. There was no significant difference in terms of gender and age between the two groups（P > 0.05）. Compared with the low copy group，the high copy group had high proportion of throat discomfort，diarrhea，and type 2 diabetes mellitus. The decrease of CD3+，CD4+ and CD8+ T lymphocytes at baseline was common in the two groups，and the CD4+ lymphocyte count in the high copy group was lower than that in the low copy group（P < 0.05）. The decrease of peripheral blood lymphocytes and eosinophils at baseline was also common in the two groups. Compared with those in the low copy group，the lymphocyte and eosinophil counts were lower and recovered slower at the first week in the high copy group（all P < 0.05）. CT value was positively correlated with lymphocytes and eosinophils at the first week（r = 0.261 and 0.333），but negatively with viral nucleic negative conversion time（r = -0.289）. Compared with that in the low copy group，the viral nucleic negative conversion time was longer in the high copy group（20.2 d vs. 16.3 d，P < 0.05），but there was no significant difference in the severe type rate between the two groups（P > 0.05）. Conclusion The early viral load of SARS⁃Cov⁃2 Delta variant is negatively correlated with peripheral blood lymphocytes and eosinophils，but positively with the time of virus negative conversion. The pathophysiological mechanism needs further study. Diabetes may affect viral load，but viral load can′t predict poor prognosis.

Objective To investigate the underlying mechanism by which long train acyl⁃CoA synthetases （ACSL4）regulates the expression of cyclooxygenase ⁃2（COX2）to promote breast cancer cell metastasis. Methods ACSL4 was overexpressed or knocked down in breast cancer cells with different invasive breast cancer phenotypes. Wound healing assay and Transwell assay were used to examine the invasive ability of cells. The relationship between ACSL4 and related genes was analyzed by using TCGA database. The regulation of COX2 by ACSL4 was verified by qPCR and Western blotting. The secretion of prostaglandin E2（PGE2）in breast cancer cells was detected by ELISA. Results In TCGA database，ACSL4 was positively correlated with COX2 expression（r = 0.15，P < 0.05）. Overexpression of ACSL4 in low⁃invasive breast cancer cells MCF⁃7 significantly increased the invasion ability of the cells and the expression of COX2 and Vimentin. Knockdown of ACSL4 in MDA⁃MB⁃231 cells significantly reduced the invasion ability of the cells and the expression of COX2 and Vimentin. ELISA assay showed that knock⁃ down of ACSL4 reduced the level of PGE2，a metabolite catalyzed by COX2，while overexpression of ACSL4 increased the level of PGE2. Conclusion ACSL4 promotes the secretion of PGE2 by increasing COX2 expression， which results in enhanced invasive potential of breast cancer cells. Our results provide a new experimental evidence and strategy for the clinical treatment of breast cancer metastasis.

Objective To explore the influencing factors of pathological complete response（pCR）by neoadjuvant chemotherapy（NAC）combined with targeted therapy for Her⁃2 positive breast cancer. Methods The clinical and pathological data of 185 patients with HER⁃2 positive breast cancer who were initially diagnosed and received NAC combined with targeted therapy were retrospectively analyzed. Univariate and multivariate analysis were used to find the factors associated with pCR. Results The overall pCR rate was 53.5%. Estrogen and proges⁃ terone receptor status，HER ⁃2 positive mode of diagnosis，and targeted therapy regimen were independent factors associated with pCR rate（P < 0.05）. Patients with hormone receptors negative were more likely to achieve pCR than positive（OR = 4.829，95%CI：2.399 ~ 9.721，P < 0.001），patients with HER⁃2 2+ and FISH positive were less likely to achieve pCR than HER⁃2 3+（OR = 0.430，95%CI：0.191 ~ 0.971，P = 0.042），and patients with dual ⁃target therapy were more likely to achieve pCR than single ⁃target therapy（OR = 2.203，95%CI：1.147 ~ 4.232，P = 0.018）. Conclusions Her⁃2 positive breast cancer patients with hormone receptor negative，Her⁃2 3+ or double target therapy are more likely to obtain pCR in NAC combined with targeted therapy. 

Objective To investigate the auxiliary positioning function of optical body surface imaging in styrofoam⁃fixed breast⁃conserving intensity⁃modulated radiotherapy and the influence of observation area on the posi⁃ tioning. Methods A total of 34 breast cancer patients who received intensity⁃modulated radiation therapy in Sun Yat⁃sen University Cancer Center from May 2020 to July 2021 were selected. The position was set according to the marking line of the body surface，and then corrected by the optical body surface monitoring system（C ⁃RAD Catalyst HD），finally the position was verified by cone beam CT（Cone Beam CT，CBCT）. The region of interest （ROI）in C⁃RAD Catalyst HD was divided into single⁃affected ROI and bilateral ROI. The treatment bed value after positioning according to the marking line，and Catalyst HD correction were recorded separately，and the set⁃up errors by CBCT were also recorded. The set⁃up errors according to the marking line of the body surface with the set⁃ up errors of Catalyst HD，the set⁃up errors of the single⁃affected side ROI with the set⁃up errors of the bilateral ROI were analyzed by t test. Results The set⁃up errors by the marking line of the body surface and C⁃RAD Catalyst HD in the left and right，head⁃foot，and abdominal⁃back directions were（2.21 ± 2.14），（2.46 ± 2.22），（3.33 ± 2.43）mm and（1.42 ± 0.99），（1.31 ± 0.86），（1.38 ± 1.06）mm respectively（LR：t = -3.473，SI：t = -4.95，AP： t = -3.438，all P < 0.05）. The three⁃dimensional linear errors of single⁃affected ROI and bilateral ROI in the left⁃ right，head⁃foot，and abdominal⁃back directions were（1.59 ± 1.23），（1.41 ± 1.02），（1.50 ± 0.93）mm and（1.71± 1.22），（1.89 ± 1.29），（1.31 ± 1.71）mm respectively；three ⁃dimensional rotation errors were［Rtn：（0.53 ± 0.49）°，Roll：（0.77 ± 0.75）°，Pitch：（0.71 ± 0.68）°］and［Rtn：（0.45 ± 0.49）°，Roll：（0.95 ± 0.75）°，Pitch： （0.71 ± 0.69）°］respectively（AP：t = 0.005，Roll：t = 0.003，P < 0.05）. Conclusion Catalyst HD can improve the positioning accuracy of intensity ⁃modulated radiotherapy after breast ⁃conserving breast cancer with assisted styrofoam fixation. It is recommended to choose bilateral ROI when selecting the region of interest monitored by the Catalyst HD.

Objective Pertuzumab（P）has been recommended as the first choice of neoadjuvant treat⁃ ment for the patients with human epidermal growth factor receptor 2（HER⁃2）positive breast cancer. There are few reports on real⁃world data in China nowadays. This study aims at investigating the clinical effects of the combination therapy based on pertuzumab in patients with HER⁃2 positive breast cancer. Methods From March 2019 to January 2022，there were 60 patients with HER⁃2 positive breast cancer who received neoadjuvant treatment with pertuzumab and underwent surgery from the Guangdong Hospital of Traditional Chinese Medicine. During the neoadjuvant treatment，We evaluated the clinical effects and postoperative pathology by magnetic resonance imaging orcomputed tomography andthe Miler⁃Payne standard. Which we used to analyze the clinical effects and adverse reaction of the combination therapy based on pertuzumab in HER⁃2 positive breast cancer patients. Results Of the 60 patients who were enrolled，the overall response rete（ORR）was 82%，the pathological effective rate（G3 + G4 + G5）was 90%，and the pathological complete response rate（pCR/G5）was 53%（32/60）. Epirubicin（e）combined with cyclophosphamide（c）sequential docetaxel（T）chemotherapy group hasthe highest pCR rate，56%（18/32）. No serious event was observed during the treatment. Conclusion In the real world，the neoadjuvant therapy combined⁃ with pertuzumab for HER⁃2 positive breast cancer patientsis safe and effective，especially combined anthracycline containing chemotherapy.

Objective To analyze the screening and influencing factors forfemale breast cancer in rural area of Nanning. Methods Breast inspection，palpation，and color Doppler ultrasound examinations were per⁃ formed. Those who were suspicious were examined by mammography. Subjects with BI ⁃RADS grade 4～5 were further diagnosed by histopathological examination. Basic characteristics of cases and controls were collected and analyzed. Results A total of 155，224 rural women were screened for breast cancer from 2017 to 2020，and the proportion of breast ultrasonography and mammography increased year by year（all P < 0.001）. A total of 211 cases were confirmed with breast cancer，with a detection rate of 135.93/105 and a decreasing trend（P < 0.001）. Invasive ductal carcinoma was the main histopathological type，accounting for 70.14 %. Stage IIA was the most common type of TNM staging，accounting for 36.42%. Significant differences in the proportion of ethnicity，age，educational level and previous inspection between cases and controls were found（all P < 0.05）. Zhuangnationality，ages 40 ~ 44，45 ~ 49，50 ~ 54，55 ~ 59，60 ~ 64，and lowereducation level were the risk factors for breast cancer，respec⁃ tively（all P < 0.05），and the highest risk of breast cancer was found in the 55~59 age group. Receiving breast can⁃ cer examinations previously was a protective factor for breast cancer. Conclusion Breast cancer screening in rural women could detect and prevent breast cancer in time. Health education for elderly or Zhuang women should be strengthened to increase their enthusiasm for screening.

Objective To investigate the diagnostic value of ultrasonography，mammography and their combination in high⁃grade and middle⁃low⁃grade ductal carcinoma in situ（DCIS）. Methods A total of 161 patients with 196 lesions who underwent ultrasound and mammography examination and confirmed by surgical pathology in our hospital between January 2015 and December 2020 were included. Taking pathological diagnosis as the gold standard，196 lesions were divided into high ⁃grade DCIS group（n = 80）and middle ⁃low ⁃grade DCIS group （n = 116）. The sonographic and mammographic features were analyzed and compared between the two groups. The area under curve（AUC）of the receiver operating characteristic（ROC）was used to analyze the diagnostic values of ultrasound and/or mammography for the DCIS. Results The percentage of microcalcification of sonography in the high⁃grade DCIS group was significantly higher than that in the middle⁃low⁃grade DCIS group（P < 0.01）. The pro⁃ portion of mass of sonography in the high ⁃grade DCIS group was significantly lower than that in the middle ⁃low ⁃ grade DCIS group（P < 0.01）. The proportion of microcalcification of mammography in the high⁃grade DCIS group was significantly higher than that in the middle⁃low⁃grade DCIS group（P = 0.026）. The AUC of ultrasound and/or mammography for DCIS diagnosis were 0.783，0.895，and 0.957，respectively. Conclusion The ultrasonographic and mammographic features of microcalcification are correlated with high⁃grade DCIS and ultrasonographic features of mass are correlated with middle⁃low⁃grade DCIS. The combination of ultrasound and mammography can help to diag⁃ nostic both high⁃grade and middle⁃low⁃grade DCIS.

Objective To explore the expression of microRNA ⁃7⁃5p（miR ⁃7⁃5p）in papillary thyroid carcinoma（PTC）tissues，para⁃carcinoma，as well as cell lines and its influenceon the proliferation，invasion and migration of PTC cells and its related molecular mechanism. Methods Quantitative Real⁃Time PCR（qRT⁃PCR） was used to detect the expression of miR⁃7⁃5p in 52 cases of PTC，para⁃carcinoma and human PTC cell lines TPC⁃1， BCPAP，K1 and human normal thyroid follicular cell line Nthy⁃ori 3⁃1，respectively. MiR⁃7⁃5p mimics and mimics ⁃NC were transfected into TPC⁃1 cells and K1cells with LipofectaminTM 3000 and divided into blank control group，negative control group（mimics⁃NC/NC⁃in group）and experimental group（mimics⁃7⁃5p/inhibitor⁃7⁃5p group）. The proliferation，invasion and migration abilities of cells in 3 groups were tested by growth curve，transwell invasion and scratch. The binding of miR ⁃7⁃5p to the TFF3⁃3′UTR was detected by dual luciferase reporter gene experi⁃ ment，and effect of miR⁃7⁃5p on TFF3 expression by western blot. Results The expression of miR⁃7⁃5p in PTC and the 3 PTC cell lines was significantly lower than that in adjacent tissues and Nthy⁃ori 3⁃1 cell（P < 0.01）. Mir⁃ 7⁃5p expression was significantly different in PTC patients with different clinical stages，tumor size and lymph node metastasis（P < 0.01）. After the up⁃regulation of miR⁃7⁃5p，the proliferation，invasion and migration abilities of TPC⁃1 and K1cells decreased significantly（P < 0.05）. The double luciferase reporter gene experiment showed that the luciferase activity value in mimics⁃7⁃5p/inhibitor⁃7⁃5p group was lower/or higher when compared that in the control group（P < 0.01）. Conclusion The expression of miR⁃7⁃5p is low in PTC tissues，and miR⁃7⁃5p can inhibit the proliferation，invasion and migration of PTC cells by targeting TFF3.

Objective To investigate the method of preparing engineered myocardial patch using skeletal muscle decellularized scaffold，and to evaluate its biological function. Methods Rat rectus abdominis was decellu⁃ larized and then cross⁃linked with EDC and NHS to obtain skeletal muscle decellularized scaffolds. Decellularization and microstructure observed while mechanical properties were determined. Biocompatibility，cardiomyocyte function⁃ alization，and endothelial cell vascularization in the skeletal muscle decellularized scaffolds were evaluated by in vitro cell assay and qRT ⁃PCR. Results As the EDC concentration was 0.5 mol/L，the most appropriate elastic modulus was found in the skeletal muscle decellularized scaffolds. The skeletal muscle decellularized scaffolds contained VEGF in each group，and the 0.5 mol/L group had the highest viable cell rate and better cytoskeleton extension in the in vitro cell assay，and the formation of intercellular sarcomere was observed after implantation of primary cardiomyocytes. Angiogenesis was seen after implantation of human umbilical vein endothelial cells，and vas⁃ cular⁃specific VEGF and eNOS proteins were verified by qRT⁃PCR. Conclusions The skeletal muscle decellularized scaffolds with cross⁃linking agent EDC 0.5 mol/L have better mechanical properties. They can accelerate maturation and functionalization of primary cardiomyocytes，and vascularization of human umbilical vein endothelial cells. 

Objective The aim of this study was to evaluate the effect and mechanism of lactulose on acute lung injury in rats with hemorrhagic shock. Methods SD rats without lactulose pretreatment were divided into three groups：sham operation group（10 rats），normal saline resuscitation group（10 rats），and lactulose treatment group（1 rats）（10 animals）ahead of 60 min before preparation of hemorrhagic shock. After the successful catheterization of the four groups of experimental animals，the hemorrhagic shock model was prepared and the vital signs were continuously monitored. The blood gas and lactic acid values were measured at 0 min，30 min and 270 min. The HMGB1 in lung tissue was detected by western method and the IL⁃6 as well as IL⁃1 in blood were detect⁃ ed by ELISA method β. SOD and MDA in lung tissue were detected by colorimetry at 270 min. The expression of MPO in lung tissue was detected by TBA at 270 min. The pathological sections of lung tissue were analyzed under light microscope. Results At 270min，the pO2 value of the control group was significantly lower than that of the blank group，experimental group 1 and experimental group 2（P < 0.05）. IL⁃6，IL⁃1 β，SOD，MDA and MPO in blank group were statistically significant different with control group，experimental group 1 and experimental group 2 respectively（P < 0.05）. The comparison between control group and experimental group 2 was also statistically significant different（P < 0.05）. Compared with experimental group 2，experimental group was also statistically significant different（P < 0.05）. HMGB1 in control group was statistically significant different with blank group， experimental group 1 and experimental group 2 respectively（P < 0.05）. The results of lung pathological sections showed that the alveolar endothelium and alveolar tissue in experimental group 1 were significantly damaged and inflammatory cell infiltration was significant obviously when comparing with experimental group 2. The destruction of alveolar endothelium and alveolar tissue and inflammatory cell infiltration in the control group were more obvious than those in experimental group 1 and experimental group 2. Conclusion Lactulose could promote the production of SOD and inhibit the production of HMGB1，and thus inhibit the release of IL⁃6，IL⁃1 β，MDA，MPO and other inflammatory mediators for protecting the lung tissue.

Objective To investigate the effects of Shikonin on proliferation and osteogenic differentiation of mouse preosteoblast cells（MC3T3⁃E1 cells）and the role of OPG/ RANKL signaling axis. Methods MC3T3⁃E1 cells were cultured in vitro and divided into control group and different concentrations of shikonin group（0.0625， 0.125，0.25，0.5，1，2 μmol/L）. After 24，48，72 h of administration，Cell proliferation inhibition rate was detected by CCK⁃8 method；ALP activity of MC3T3⁃E1 cells was detected by alkaline phosphatase（ALP）activity kit. The NBT/BCIP kit was used to identify ALP staining. The expression levels of osteoprotegerin（OPG），nuclear factor κB receptor activating factor ligand（RANKL），Runt ⁃associated transcription factor 2（RUNX2）and type ⁃ I collagen （COL ⁃ I）genes were detected by Real ⁃time fluorescence quantitative PCR（RT ⁃PCR）；Alizarin red staining was used to observe the formation of mineralized nodules. Results Compared with the control group，0.0625，0.125， 0.25 and 0.5 μmol/L shikonin groups significantly promoted the proliferation of MC3T3⁃E1 cells（P < 0.05），while 0.5 μmol/L shikonin group significantly inhibited the growth of MC3T3⁃E1 cells（P < 0.05）. The safe concentration of shikonin group promoted the proliferation，differentiation and mineralization of osteoblasts in a concentration⁃ dependent manner. RT⁃PCR results showed that，when compared to the control group，the safe Shikonin concentra⁃ tion significantly increased the expression of OPG，RUNX2，and COL⁃I while inhibiting the expression of RANKL （P < 0.05）. Conclusions Shikonin can promote the proliferation，differentiation and mineralization of MC3T3⁃E1 cells，possibly by regulating the OPG/ RANKL signaling axis and osteogenic genes.

Objective To explore the value of high⁃resolution musculoskeletal ultrasound（MSUS）gray⁃ scale（GS）in judging the chronic Achilles tendinitis（CAT）and to find the association between potential clinical risk factors and CAT. Methods A total of 88 ice and snow athletes with CAT that underwent gray scale ultrasound in our hospital were included in the study. The differences in gray scale ultrasound characteristic between the affected side，the healthy area around the affected side，and the healthy side achilles tendon were compared. Results The average gray scale of the inflammatory area was lower than that of the Achilles tendon in the healthy area（either ipsilateral or contralateral）（P < 0.05）. However，there was no significant difference between healthy areas around inflammatory areas and contralateral healthy areas（P > 0.05）. Multivariate logistic analysis revealed that age （> 30 years），training time（> 5 years），and achilles tendon tension test were significantly associated with the decrease of average gray scale and max gray scale of inflammatory area. Conclusion Gray scale ultrasound has certain application value in the diagnosis of CAT when combined with clinical data.

Objective To compare the efficacy and safety of different chemotherapy regimens used in TACE for the treatment of primary liver cancer. Methods 136 patients with primary liver cancer who had received transcatheter arterial chemoembolization（TACE）from January 2012 to January 2019 in our center were selected. Patients were divided into group A and group B according to different chemotherapy regimens in TACE. Group A received chemotherapy regimen with fluorouracil 1 000 mg plus oxaliplatin 100 mg and pirarubicin 40 mg，which was partially emulsified with lipiodol and then for embolization，and partially perfused through superior mesenteric artery and hepatic artery. In group B，oxaliplatin 100 mg was emulsified with lipiodol for embolization. Objective re⁃ sponse rate（ORR），disease control rate（DCR），and progression⁃free survival（PFS）were used to evaluate the effectiveness of the therapies；the incidence of serious complications was used to assess the safety. Results A total of 136 patients met the inclusion criteria，including 98 in group A and 38 in group B. There were no significant differences in the baseline characteristics between the two groups. The ORR was 53.1 %（52/98）in group A and 36.8%（14/38）in group B，and the difference was not statistically significant（χ2 = 2.884，P = 0.089）. The DCR was 82.7 %（81/98）in group A and 71.1 %（27/38）in group B，and the difference was not statistically significant （χ2 = 2.254，P = 0.133）. The median PFS was 192 days（95%CI：152~231）in group A and 183 days（95%CI： 60~305）in group B，and the difference was not statistically significant（χ2 = 1.081，P = 0.299）. Multivariate regression analysis showed that the only independent risk factor for PFS was extrahepatic metastasis（HR = 2.283， 95%CI：0.25 ~ 0.77，P = 0.004）. The incidence of serious adverse reactions was 15.3 %（15/98）in group A and 2.6%（1/38）in group B，and the difference was statistically significant（P = 0.04）. Conclusions The efficacy of TACE with low⁃dose monotherapy for primary liver cancer is the same as that of TACE with high⁃dose polytherapy. For the low⁃dose monotherapy，the incidence of complications is reduced and the safety of therapies is improved.

Objective To compare the efficacy of percutaneous kyphoplasty（PKP）with that of percutaneous vertebroplasty（PVP）in the treatment of osteoporotic thoracic vertebral fractures with intercostal pain. Methods A total of 86 patients with thoracic OVCFs and intercostal pain，who had admitted to our hospital from January 2020 to February 2021，were selected and randomly divided into two groups. The PKP group received PKP after postural reduction，while the PVP group underwent PVP alone. Both groups were followed up for 6 months. Surgical duration，length of hospital stay，bone cement injection volume，height ratio of the anterior edge of the impaired vertebrae，kyphotic angle，VAS score，ODI score，and related complications were recorded in the two groups. Results The VAS scores of postoperative intercostal pain and chest and back pain in both groups were significantly improved as compared with the baselines，and the difference was statistically significant（P < 0.05）. The postoper⁃ ative ODI in both groups was significantly improved as compared with the baseline，and the difference was statisti⁃ cally significant（P < 0.05）. The height ratio of the anterior edge of the impaired vertebrae and the kyphotic angle were significantly improved in the PKP group after the procedure，and the difference was statistically significant （P < 0.05）；while the PVP group was improved after the procedure，and the difference was not statistically signifi⁃ cant（P > 0.05）. There were no significant statistical differences between the two groups in surgical duration，length of hospital stay，VAS score and ODI score. The PKP group was better than the PVP group in bone cement injection volume，bone cement leakage，height ratio of the anterior edge of the impaired vertebra，and kyphotic angle of the impaired vertebra. Conclusions Both PKP following postural reduction and PVP alone can effectively treat thoracic OVCFs with intercostal pain. The former is significantly better than the latter in bone cement leakage and recovery of impaired vertebral height and kyphotic angle.

Objective To explore the correlation and consistency between the thromboelastogram and traditional coagulation tests in ordinary hospitalized patients. Method A retrospective review of 886 inpatients in our center who met the admission criteria and underwent thrombelastography（TEG）examinations between January 2018 and December 2020. Collecting platelet count，coagulation tests and thromboelastogram，and analyzing the correlation and consistency of the two detection methods. Result A value is significantly correlated with PLT，D⁃ dimer，FIB，among which is the strongest correlation with FIB（r = 0.486，P < 0.001）；CI has significant correlation with PLT，D⁃dimer，PT，FIB，APTT，TT ，Among which the correlation with FIB is the strongest（r = 0.362，P < 0.001）；G has significant correlation with PLT and FIB，among which the correlation with FIB is the strongest （r = 0.181，P < 0.001）；MA has the strongest correlation with PLT，D⁃dimer，PT，FIB，APTT have significant correlation，of which the correlation with FIB is the strongest（r = 0.380，P < 0.001）；Angle has a significant correlation with PLT，D⁃dimer，FIB，APTT，and the correlation with FIB is the strongest（r = 0.380，P < 0.001）； K value has significant correlation with PLT，D⁃dimer，PT，FIB，APTT，among which the correlation with FIB is the strongest（r = -0.251，P < 0.001）；D⁃dimer and PLT ，K，Angle，MA，CI，A have significant correlations， among which the correlation with MA value is the strongest（r = 0.163，P < 0.001）；PLT has significant correlation with D⁃dimer，K，Angle，MA，CI，A Among them，the correlation with the MA value has the strongest（r = 0.483， P < 0.001）. When assessing hypercoagulability status，PT is consistent with PLT，R⁃time，K，and MA，PLT is consistent with K，Angle，and CI，FIB is consistent with PLT，K，Angle，and MA，and APTT is consistent with PLT，K，MA，CI；When evaluating hypocoagulation status，FIB is consistent with PLT，K，Angle，MA，and CI，and PLT is consistent with R⁃time，K，Angle，MA，and CI. Other indicators′ consistency was not statistically significant. Conclusion There is a correlation between thrombosis attempts and traditional coagulation tests in ordinary hospi⁃ talized patients，but the consistency of the blood hypocoagulation or hypercoagulation status judgment is poor. TEG more accurately reflects the coagulation and fibrinolysis processes，but coagulation tests are more sensitive to slight chang⁃ es in blood. The two test methods can be used in combination to provide a more comprehensive coagulation situation.

Objective To analyze the risk factors for interstitial lung disease （ILD）in hospitalized patients with polymyositis（PM）or dermatomyositis（DM）and to establish a clinical prediction model. Methods 379 in patients with PM or DM in Tangdu Hospital of Air Force Military Medical University from January 1，2015 to January 1，2021 were included in the study and they were divided into a control group（PM/DM）and a case group（PM/DM⁃ILD）. The clinical data on the patients were retrospectively collected. The risk factors of PM/DM⁃ ILD were summarized by binary logistic regression. A clinical prediction model was constructed by using the R software. Results The patients′ age was older；the incidences of joint pain，fever，mechanic′s hand，Raynaud′s phenomenon，chest tightness，shortness of breath，cough and expectoration were greater；and erythrocyte sedimen⁃ tation rate（ESR），serum ferritin（FER）and levels of carcinoembryonic antigen（CEA），carbohydrate antigen 153（CA153），anti⁃Jo⁃1 antibody，anti⁃Ro52 antibody and anti⁃mda5 antibody were higher in the control group than in the control group；while level of serum albumin（ALB）was lower（P < 0.05）. Binary logistic regression analysis showed that age，fever，mechanic′s hand，Reynolds phenomenon，FER，CEA，serum albumin（ALB）， anti⁃Jo⁃1 antibody，anti⁃Ro52 antibody and anti⁃mda5 antibody were the risk factors of ILD（P < 0.05）. The AUC of the constructed clinical prediction model was 0.899（95%CI：0.864 ~ 0.934）. The sensitivity was 79.5% and the specificity was 86.4%. Conclusions The risk factors of PM/DM⁃ILD are old age，fever，mechanic′s hand，Reyn⁃ olds phenomenon，increased levels of FER and CEA，a decrease in serum albumin（ALB），and positive anti⁃Jo⁃1， anti⁃Ro52 and anti⁃mda5 antibodies. The model constructed in this study can better predict the occurrence of PM/ DM⁃ILD，and it has certain reference value for early clinical prevention and treatment.

Objective To investigate the dose⁃response relationship of sugammadex sodium to reverse the neuromuscular block effect of rocuronium and calculate the 50% effective dose（ED50）and 95% effective dose （ED95）of sugammadex sodium. Methods A total of 50 adult patients who planned to undergo elective ear，nose， and throat surgery in our hospital from June 2020 to June 2021 were selected. Age 18 ~ 65 years，ASA GRADE Ⅰ~ Ⅱ，body mass index（BMI）18.5 ~ 27.9 kg/m2，ear，nose，and throat surgery performed under general anesthesia， and expected surgical duration > 90 minutes are all inclusion criteria. Dixon sequential method was used to adjust the dose of sugammadex sodium. The first patient received an initial intravenous dose of sugammadex sodium 4 mg/kg with a dose gradient of 0.5 mg/kg. If the neuromuscular block effect of rocuronium was successfully reversed in the previous patient，the dose of sugammadex sodium was reduced by a gradient in the next patient；If the neuromuscular blocking effect of rocuronium was not reversed in the previous patient ，the dose of sugammadex sodium was increased by a gradient in the next patient. Follow the preceding rules until 50 patients have been included. In all patients，the success or failure of recovery from neuromuscular block was recorded. HR and MAP were measured prior to，immediately following，1 minute，2 minutes，and 5 minutes after sugammadex sodium injection. Following extubation，the patient was transferred to the PACU for 1 hour of observation，during which time the occurrences of hypotension，bradycardia，nausea and vomiting，dizziness，and allergic reactions were recorded. Results The ED50 and ED95 of sugammadex sodium to reverse the neuromuscular block effect of rocuronium were 3.481 mg/kg （95%CI：3.310 ~ 3.652）and 4.178 mg/kg（95%CI：3.769 ~ 4.587）. Patients were divided into sugammadex sodium 4 mg/kg group（S4 group）sugammadex sodium 3.5 mg/kg group（S3.5 group）and sugammadex sodium 3 mg/kg group（S3 group）according to the specific value of ED50 and ED95 and sample size of each dose，each group includ⁃ ed 12，22 and 13 patients，respectively. There were no significant differences in HR and MAP before，immediate⁃ ly after，1 min，2 min and 5 min after sugammadex sodium injection among the three groups（P > 0.05）. There were no significant differences in HR and MAP between the S4 and S3 groups at any time point（P > 0.05）. The HR of patients in S3.5 group at 1 min，2 min and 5 min after injection was significantly lower than that of before injec⁃ tion，with statistical significance（P < 0.05）. There was no significant difference in the incidence of hypotension， bradycardia，nausea and vomiting，dizziness，and allergic reaction among 3 groups（P > 0.05）. Conclusion Dif⁃ ferent doses of sugammadex sodium can reverse the neuromuscular block effect of rocuronium. The larger the dose， the stronger the block effect. At the same time，attention should be paid to prevent adverse reactions such as bra⁃ dycardia during medication.

Objective The aim of this study was to construct cationic liposome based CLPs⁃DOX platform for cancer therapy and imaging. Methods In this experiment，CLPs⁃DOX was synthesized by thin film dispersion method. Zeta potential，size distribution and fluorescence of CLPs⁃DOX were analyzed by potentiometric analyzer， fluorescence analyzer，nanoparticle tracking technology. The imaging effect，cytotoxicity of CLPs ⁃DOX on breast cancer cells MDA⁃MB⁃231 were also investigated by fluorescence microscopy，confocal microscopy and CCK⁃8 assay. Results DOX encapsulated efficiency of one ⁃pot synthesis method was higher than two ⁃step method. The fluorescence response value of DOX loaded on CLPs increased after Triton X⁃100 treatment. The fluorescence peaks of DOX and CLPs⁃DOX were both 590 nm. Cancer cells mortality rate increased to 20% in the addition of 1 × 108 particles CLPs⁃DOX. CLPs⁃DOX showed a good imaging effect in breast cancer cells MDA⁃MB⁃ 231. Conclusion The synthesized CLPs⁃DOX exhibits desirable cytotoxicity and imaging capacity with uniform size distribution and high loading efficiency，paving the way to clinical practice.

Eosinophils play an important role in the development of allergic diseases. This paper aims to describe the relationships between eosinophils and allergic diseases in terms of eosinophil maturation，migration， activation，degranulation and delayed apoptosis，elaborating their role in three different allergic diseases. By study⁃ ing the biological characteristics of eosinophils and their roles in allergic diseases，we can find more directions for the treatment of allergic diseases in order to guide the clinical diagnosis.

Chronic liver disease is a significant global health burden，accounting for 3.5% of all deathsworldwide each year. Serum endotoxin level is closely related to end ⁃stage liver disease ，which can cause oraggravate liver injury in direct or indirect way. However，the specific role of endotoxemia in liver injury is not yetclear. The latest gut⁃liver axis related studies found that the role of endotoxin in the progression of liver injurymay be seriously underestimated. Therefore，this article focuses on the mechanism and role of endotoxemia in theoccurrence and development of chronic liver disease，thus to provide theoretical basis for clinical guidance for thetreatment of chronic liver disease.