The Journal of Practical Medicine >

2026 , Vol. 42 >Issue 7: 1201 - 1207

DOI: https://doi.org/10.3969/j.issn.1006-5725.2026.07.012

Oncology: Diagnosis, Treatment and Prevention

Predictive analysis of serum COL10A1， miRNA-21 and miRNA-320c expression levels for peritoneal metastasis and prognosis of gastric cancer

  • Fengxue DAI ,
  • Zhenfei WANG
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  • 1.Department of Oncology，Affiliated Cancer Hospital of Inner Mongolia Medical University，Hohhot 010020，Inner Mongolia，Chin
    a2Research Laboratory for the Inheritance and Development of Integrated Traditional Chinese and Western Medicine in Anti-Tumor Studies，Affiliated Cancer Hospital of Inner Mongolia Medical University，Hohhot 010020，Inner Mongolia，China

Received date: 2025-12-07

  Revised date: 2026-01-21

  Accepted date: 2026-01-23

  Online published: 2026-04-13

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Abstract

Objective To explore the predictive efficacy of serum expression levels of collagen type X alpha 1 （COL10A1）， microRNA （miRNA）-21， and miRNA-320c in peritoneal metastasis and prognosis of gastric cancer. Methods The clinical data of 120 patients diagnosed with gastric cancer and admitted to our hospital between July 2023 and September 2024 were retrospectively analyzed. Post-treatment， the prognostic outcomes of these patients were tracked through outpatient and telephone follow-up. Based on the occurrence of peritoneal metastasis， the patients were classified into the peritoneal metastasis group and the non-peritoneal metastasis group. According to the prognostic outcomes， they were further divided into the poor prognosis group and the good prognosis group. Enzyme-linked immunosorbent assay （ELISA） was employed to detect the expression of serum COL10A1. Quantitative real-time polymerase chain reaction （qRT-PCR） was utilized to measure the expression levels of miRNA-21 and miRNA-320c. A comparison was made regarding the clinical characteristics， serum levels of COL10A1， miRNA-21， and miRNA-320c between the peritoneal metastasis group and the non-peritoneal metastasis group. Additionally， the serum expression levels of the aforementioned indicators were also compared among gastric cancer patients with different prognostic outcomes. Cox univariate and multivariate stepwise regression analyses were carried out to identify the factors influencing the prognosis of gastric cancer. Receiver operating characteristic （ROC） curve analysis was performed to assess the predictive efficacy of serum COL10A1， miRNA-21， and miRNA-320c for the prognosis of gastric cancer. Results Among the 120 gastric cancer patients， 31 cases presented with peritoneal metastasis while 89 cases did not. The TNM stage of the peritoneal metastasis group was notably higher than that of the non-peritoneal metastasis group （P < 0.05）. The serum expression levels of COL10A1， miRNA-21， and miRNA-320c in the peritoneal metastasis group were all elevated compared to those in the non-peritoneal metastasis group （P < 0.05）. Out of the 120 patients， 42 cases belonged to the poor prognosis group and 78 cases to the good prognosis group. The serum levels of COL10A1， miRNA-21， and miRNA-320c in the poor prognosis group were significantly higher than those in the good prognosis group （P < 0.05）. Cox univariate analysis demonstrated that TNM stage， peritoneal metastasis， lymph node metastasis， serum levels of COL10A1， miRNA-21， and miRNA-320c were all associated with the prognosis of gastric cancer patients （all P < 0.05）； multivariate analysis disclosed that TNM stage， peritoneal metastasis， serum levels of COL10A1， miRNA-21， and miRNA-320c were independent risk factors influencing the prognosis of gastric cancer patients （all P < 0.05）. ROC curve analysis suggested that the combined detection of serum COL10A1， miRNA-21， and miRNA-320c for predicting the prognostic outcomes of gastric cancer had a sensitivity of 89.45% and a specificity of 92.34%， with the area under the curve （AUC） reaching 0.921， which was significantly higher than that of single detection of COL10A1 （P = 0.011）， miRNA-21 （P = 0.023）， and miRNA-320c （P = 0.015）. Conclusion Serum COL10A1， miRNA-21， and miRNA-320c are highly expressed in gastric cancer patients with peritoneal metastasis and poor prognosis. These indicators are independent risk factors for the poor prognosis of gastric cancer patients， and their combined application demonstrates high predictive efficacy for the prognostic outcomes of gastric cancer patients.

Key words： gastric cancer; peritoneal metastasis; prognosis; serum COL10A1; miRNA-21; miRNA-320c; predictive efficacy

Cite this article

Fengxue DAI , Zhenfei WANG . Predictive analysis of serum COL10A1， miRNA-21 and miRNA-320c expression levels for peritoneal metastasis and prognosis of gastric cancer[J]. The Journal of Practical Medicine, 2026 , 42(7) : 1201 -1207 . DOI: 10.3969/j.issn.1006-5725.2026.07.012

References

[1] YANG W J， ZHAO H P， YU Y， et al. Updates on global epidemiology， risk and prognostic factors of gastric cancer［J］. World J Gastroenterol， 2023， 29（16）： 2452-2468. doi： 10.3748/wjg.v29.i16.2452 .
[2] 成晓芬， 闵淑慧， 郭芮绮， 等. 1990-2019年中国胃癌发病与死亡率年龄-时期-队列分析及2020-2030年变化趋势预测［J］. 中国肿瘤， 2023， 32（6）： 454-461. doi： 10.11735/j.issn.1004-0242.2023.06.A008 .
[3] HUANG X Z， PANG M J， LI J Y， et al. Single-cell sequencing of ascites fluid illustrates heterogeneity and therapy-induced evolution during gastric cancer peritoneal metastasis［J］. Nat Commun， 2023， 14（1）： 822. doi： 10.1038/s41467-023-36310-9 .
[4] LI Z， WANG J， WANG Z， et al. Towards an optimal model for gastric cancer peritoneal metastasis： Current challenges and future directions［J］. EBioMedicine， 2023， 92： 104601. doi： 10.1016/j.ebiom.2023.104601 .
[5] NAGARAJAN A， VARADHAN V， MANIKANDAN M S， et al. Signature of collagen alpha-1（x） gene expression in human cancers and their therapeutic implications［J］. Pathol Res Pract， 2025， 266： 155811. doi： 10.1016/j.prp.2025.155811 .
[6] SEO S B， HWANG J S， KIM E， et al. Isothermal amplification-mediated lateral flow biosensors for in vitro diagnosis of gastric cancer-related microRNAs［J］. Talanta， 2022， 246： 123502. doi： 10.1016/j.talanta.2022.123502 .
[7] FANG J， SUN Y， YU Y， et al. Plasma Small RNAs as Predictive and Monitoring Biomarkers for Combination Immunotherapy in Advanced Gastric Cancer［J］. Cancer Med， 2025， 14（21）： e71339. doi： 10.1002/cam4.71339 .
[8] MENG Z， ZHANG R， WU X， et al. LncRNA HAGLROS promotes breast cancer evolution through miR-135b-3p/COL10A1 axis and exosome-mediated macrophage M2 polarization［J］. Cell Death Dis， 2024， 15（8）： 633. doi： 10.1038/s41419-024-07020-x .
[9] 中华医学会肿瘤学分会， 中华医学会杂志社. 中华医学会胃癌临床诊疗指南（2021版）［J］. 中华医学杂志， 2022， 102（16）： 1169-1189. doi： 10.3760/cma.j.cn112137-20220127-00197 .
[10] 中国抗癌协会胃癌专业委员会. 胃癌腹膜转移诊治中国专家共识（2023版）［J］. 中华胃肠外科杂志， 2023， 26（8）： 717-728. doi： 10.3760/cma.j.cn441530-20230608-00196 .
[11] MATSUOKA T， YASHIRO M. Bioinformatics Analysis and Validation of Potential Markers Associated with Prediction and Prognosis of Gastric Cancer［J］. Int J Mol Sci， 2024， 25（11）： 5880. doi： 10.3390/ijms25115880 .
[12] WANG X， BAI Y， ZHANG F， et al. Prognostic value of COL10A1 and its correlation with tumor-infiltrating immune cells in urothelial bladder cancer： A comprehensive study based on bioinformatics and clinical analysis validation［J］. Front Immunol， 2023， 14： 955949. doi： 10.3389/fimmu.2023.955949 .
[13] NECULA L G， DRAGU D L， MATEI L， et al. COL10A1 Overexpression Promotes Gastric Cancer Aggressiveness Through EMT and Major Oncogenic Pathways［J］. Int J Mol Sci， 2025， 26（22）： 11043. doi： 10.3390/ijms262211043 .
[14] SHEN N， ZHU S， ZHANG Z， et al. High Expression of COL10A1 Is an Independent Predictive Poor Prognostic Biomarker and Associated with Immune Infiltration in Advanced Gastric Cancer Microenvironment［J］. J Oncol， 2022： 1463316. doi： 10.1155/2022/1463316 .
[15] 王佩显， 单彪， 张倩倩， 等. 血清COL10A1、TK1、MIP-3α水平与胃癌患者病理特征的关系及其对腹膜转移的诊断价值研究［J］. 检验医学与临床， 2024， 21（12）： 1727-1730， 1738. doi： 10.3969/j.issn.1672-9455.2024.12.012 .
[16] FARASATI FAR B， VAKILI K， FATHI M， et al. The role of microRNA-21 （miR-21） in pathogenesis， diagnosis， and prognosis of gastrointestinal cancers： A review［J］. Life Sci， 2023， 316： 121340. doi： 10.1016/j.lfs.2022.121340 .
[17] 王艳花， 聂亚楠， 齐俊娟， 等. miR-21靶向PTEN调控AKT/FoxO1信号通路对胃癌细胞凋亡的机制研究［J］. 河北医学， 2023， 29（12）： 1985-1992. doi： 10.3969/j.issn.1006-6233.2023.12.010 .
[18] SILVIA LIMA R Q D， VASCONCELOS C F M， GOMES J P A， et al. miRNA-21， an oncomiR that regulates cell proliferation， migration， invasion and therapy response in lung cancer［J］. Pathol Res Pract， 2024， 263： 155601. doi： 10.1016/j.prp.2024.155601 .
[19] HAN X， LV C. MiRNA-21 promotes the migration and proliferation of prostate cancer cells via activating the JAK/STAT pathway［J］. Discov Oncol， 2025， 16（1）： 162. doi： 10.1007/s12672-025-01883-y .
[20] N?ST T H， SKOGHOLT A H， URBAROVA I， et al. Increased levels of microRNA-320 in blood serum and plasma is associated with imminent and advanced lung cancer［J］. Mol Oncol， 2023， 17（2）： 312-327. doi： 10.1002/1878-0261.13336 .
[21] YANG C K， HSU H C， LIU Y H， et al. EV-miRome-wide profiling uncovers miR-320c for detecting metastatic colorectal cancer and monitoring the therapeutic response［J］. Cell Oncol （Dordr）， 2022， 45（4）： 621-638. doi： 10.1007/s13402-022-00688-3 .
[22] 李蓉， 缪忠惠， 唐嘉黛， 等. miR-320c靶向FADD基因抑制肺腺癌细胞凋亡促进癌细胞的迁移和侵袭［J］. 现代肿瘤医学， 2022， 30（9）： 1531-1537. doi： 10.3969/j.issn.1672-4992.2022.09.001 .
[23] LIU Q， ZHAO H， GUO Y， et al. Bioinformatics-Based Analysis： Noncoding RNA-Mediated COL10A1 Is Associated with Poor Prognosis and Immune Cell Infiltration in Pancreatic Cancer［J］. J Healthc Eng， 2022： 7904982. doi： 10.1155/2022/7904982 .
[24] WANG H F， WANG H H， YE X J， et al. Therapeutic effects and safety of apatinib mesylate on patients with gastric carcinoma peritoneal metastasis in SOX scheme［J］. Eur Rev Med Pharmacol Sci， 2023， 27（14）： 6627-6638. doi： 10.26355/eurrev_202307_33133 .
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