The Journal of Practical Medicine >

2026 , Vol. 42 >Issue 3: 363 - 370

DOI: https://doi.org/10.3969/j.issn.1006-5725.2026.03.002

Feature Reports：Bone and joint

Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthritis treated with H2O2

  • Lixia ZHANG ,
  • Xuefei FAN ,
  • Suhuan CHEN ,
  • Mengyan ZHANG ,
  • Yubao SHAO ,
  • Jian ZHOU ,
  • Xiaoyu CHEN
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  • 1.Department of Orthopedics，the First Affiliated Hospital of Anhui Medical University，Hefei 230031，Anhui，China
    2.School of Basic Medical Sciences，Anhui Medical University，Hefei 230032，Anhui，China

Received date: 2025-09-11

  Online published: 2026-02-09

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Abstract

Objective To investigate the effect of resveratrol （Res） on fibroblast-like synovial cells of osteoarthritis treated with H2O2， and to clarify the regulatory mechanism relationship of the PINK1/Parkin mitochondrial autophagy signaling pathway in the above effects. Methods Fibroblast-like synovial cells （FLSs） were extracted from the synovial membranes of patients undergoing osteoarthritis surgery and divided into the control group and the experimental group. The experimental group was treated with H2O2 （5 μmol/L） and then treated with different concentrations of Res （0， 10， 20， 40， 60， 80， 100 μmol/L）. Cell viability assay （CCK-8） was used to detect the inhibition rate of cell proliferation. Cell reactive oxygen species were detected by DCFH-DA fluorescent probe. Mitochondrial reactive oxygen species were detected by MitoSOX Red fluorescent probe. Cell cycle was detected by flow cytometry. The expressions of proteins such as NLRP3， TNF-α， IL-1β， SOD2， Bax， Bcl-2， BNIP3， LC3B， p62， PINK1 and Parkin were detected by Western blot and immunofluorescence experiments. The role of the PINK1/Parkin mitochondrial autophagy signaling pathway in Res' inhibition of abnormal proliferation， inflammation and oxidative stress in FLSs was further verified using PINK1 and Parkin interfering RNA. Results Res significantly inhibited the enhancement of FLSs activity induced by H2O2， and reduced the abnormal proliferation， inflammation and oxidative stress levels of FLSs； Res inhibited the expressions of NLRP3， TNF-α， Bcl-2 and p62 proteins， while promoting the expressions of SOD2， Bax， BNIP3， LC3B， PINK1 and Parkin proteins. H2O2 inhibited the PINK1/Parkin signaling pathway， while Res activated the PINK1/Parkin pathway. The use of PINK1 and Parkin interfering RNA exacerbated cellular inflammation and oxidative stress. Conclusion Res activates the PINK1/Parkin mitochondrial autophagy signaling pathway by increasing the expression of PINK1 and Parkin proteins， alleviating abnormal proliferation， inflammation and oxidative stress in fibroblast-like synovial cells of osteoarthritis induced by H2O2.

Key words： resveratrol; osteoarthritis; fibroblast-like synovial cells; PINK1/Parkin signaling pathway; inflammation; oxidative stress

Cite this article

Lixia ZHANG , Xuefei FAN , Suhuan CHEN , Mengyan ZHANG , Yubao SHAO , Jian ZHOU , Xiaoyu CHEN . Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthritis treated with H2O2[J]. The Journal of Practical Medicine, 2026 , 42(3) : 363 -370 . DOI: 10.3969/j.issn.1006-5725.2026.03.002

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