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The effect of LCN2⁃mediated EGFR phosphorylation on inflammatory injury in human fallopian tube epithelial cells
Received date: 2025-04-12
Online published: 2025-07-29
Objective To investigate the regulatory effect of lipocalin 2 （LCN2） on epidermal growth factor receptor （EGFR） phosphorylation and its impact on inflammatory damage in human fallopian tube epithelial cells. Methods Human fallopian tube epithelial cells were isolated and a lipopolysaccharide （LPS） intervention was applied to establish an in vitro cell model. The cells were randomly assigned into the following groups： a blank control group （Control）， a model group （Model）， experimental groups （Model + si-LCN2 or Model + oe-LCN2）， and negative control groups （Model + si-NC or Model + oe-NC）. Changes in cell viability， apoptosis rates， inflammatory levels， as well as the expression of EGFR mRNA， LCN2， EGFR， p-EGFR， and the ratio of p-EGFR/EGFR proteins were evaluated. Results Compared to the Model group， the Model + si-LCN2 group exhibited enhanced cell viability， a reduced apoptosis rate， and decreased expression of inflammatory factors （P < 0.05）. Immunoprecipitation analysis confirmed a direct interaction between LCN2 and EGFR. In comparison with the Model group， the Model + oe-LCN2 group demonstrated elevated levels of p-EGFR and the p-EGFR/EGFR ratio （P < 0.05）， while no significant change was observed in total EGFR expression （P > 0.05）. Conclusion Inhibition of LCN2-mediated EGFR phosphorylation enhances cell viability， reduces apoptosis， and mitigates inflammatory responses， thereby ameliorating LPS-induced inflammatory injury in human fallopian tube epithelial cells.
Yaqiong XIA , Yue ZHU , Chengcheng JIANG , Hongping TAN , Yan ZHANG , Ling. LIU . The effect of LCN2⁃mediated EGFR phosphorylation on inflammatory injury in human fallopian tube epithelial cells[J]. The Journal of Practical Medicine, 2025 , 41(14) : 2174 -2182 . DOI: 10.3969/j.issn.1006-5725.2025.14.008
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