LCN2介导EGFR磷酸化对人输卵管上皮细胞炎性损伤的影响

doi:10.3969/j.issn.1006-5725.2025.14.008

Abstract

Abstract:

Objective To investigate the regulatory effect of lipocalin 2 （LCN2） on epidermal growth factor receptor （EGFR） phosphorylation and its impact on inflammatory damage in human fallopian tube epithelial cells. Methods Human fallopian tube epithelial cells were isolated and a lipopolysaccharide （LPS） intervention was applied to establish an in vitro cell model. The cells were randomly assigned into the following groups： a blank control group （Control）， a model group （Model）， experimental groups （Model + si-LCN2 or Model + oe-LCN2）， and negative control groups （Model + si-NC or Model + oe-NC）. Changes in cell viability， apoptosis rates， inflammatory levels， as well as the expression of EGFR mRNA， LCN2， EGFR， p-EGFR， and the ratio of p-EGFR/EGFR proteins were evaluated. Results Compared to the Model group， the Model + si-LCN2 group exhibited enhanced cell viability， a reduced apoptosis rate， and decreased expression of inflammatory factors （P < 0.05）. Immunoprecipitation analysis confirmed a direct interaction between LCN2 and EGFR. In comparison with the Model group， the Model + oe-LCN2 group demonstrated elevated levels of p-EGFR and the p-EGFR/EGFR ratio （P < 0.05）， while no significant change was observed in total EGFR expression （P > 0.05）. Conclusion Inhibition of LCN2-mediated EGFR phosphorylation enhances cell viability， reduces apoptosis， and mitigates inflammatory responses， thereby ameliorating LPS-induced inflammatory injury in human fallopian tube epithelial cells.

Key words: LCN2, inflammatory damage of human fallopian tube epithelial cells, EGFR, EGFR phosphorylation

CLC Number:

R715.2

Yaqiong XIA,Yue ZHU,Chengcheng JIANG,Hongping TAN,Yan ZHANG,Ling. LIU. The effect of LCN2⁃mediated EGFR phosphorylation on inflammatory injury in human fallopian tube epithelial cells[J]. The Journal of Practical Medicine, 2025, 41(14): 2174-2182.

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References 28

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组别	样本量	0 h	12 h	24 h
Control组	3	0.94 ± 0.05	1.21 ± 0.03	1.74 ± 0.04
Model组	3	0.93 ± 0.04	0.95 ± 0.04^△	1.28 ± 0.03^△
Model+si-NC组	3	0.95 ± 0.03	0.96 ± 0.03^△	1.27 ± 0.03^△
Model+si-LCN2组	3	0.94 ± 0.03	1.04 ± 0.04^△*	1.36 ± 0.04^△
F值		0.06	38.22	115.16
P值		0.981	< 0.001	< 0.001

组别	样本量	UR晚期凋亡	LR早期凋亡	凋亡（UR+LR）
Control组	3	0.40 ± 0.27	1.50 ± 0.19	1.90 ± 0.43
Model组	3	16.20 ± 0.36^△	5.59 ± 0.82^△	21.79 ± 0.59^△
Model+si-NC组	3	19.33 ± 0.36^△?	4.07 ± 0.22^△?	23.40 ± 0.53^△?
Model+si-LCN2组	3	5.69 ± 0.40^△?#	2.95 ± 0.35^△?	8.64 ± 0.73^△?#
F值		1931.08	40.95	955.78
P值		< 0.001	< 0.001	< 0.001

组别	IL-1β	IL-6	IL-8	TNF-α
Control组	18.92 ± 0.70	40.83 ± 3.63	61.64 ± 4.03	37.11 ± 1.87
Model组	87.97 ± 1.81^△	108.66 ± 3.54^△	121.24 ± 6.82^△	90.64 ± 3.68^△
Model+si-NC组	89.15 ± 3.43^△	111.11 ± 5.70^△	124.58 ± 5.48^△	88.50 ± 2.56^△
Model+si-LCN2组	45.11 ± 1.96^△?#	80.28 ± 3.87^△?#	82.22 ± 4.40^△?#	52.07 ± 3.47^△?#
F值	730.18	175.71	100.45	239.41
P值	< 0.001	< 0.001	< 0.001	< 0.001

组别	样本量	LCN2	EGFR	p-EGFR	p-EGFR/EGFR
Control组	3	12.84 ± 0.92	68.83 ± 2.55	9.13 ± 0.96	0.13 ± 0.01
Model组	3	52.52 ± 1.75^△	66.55 ± 2.01	34.58 ± 2.32^△	0.52 ± 0.02^△
Model+oe-NC组	3	61.69 ± 1.90^△?	74.84 ± 2.79^?	33.29 ± 2.03^△	0.45 ± 0.02^△?
Model+oe-LCN2组	3	79.40 ± 2.10^△?#	71.41 ± 2.21	48.48 ± 3.42^△?#	0.68 ± 0.03^△?#
F值		796.67	6.56	144.96	379.17
P值		< 0.001	0.015	< 0.001	< 0.001

The effect of LCN2⁃mediated EGFR phosphorylation on inflammatory injury in human fallopian tube epithelial cells

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