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Effects of miR⁃223 on prostate cancer cell damage by regulating Keap1/Nrf2/ARE signaling pathway
Received date: 2023-09-05
Online published: 2024-09-13
Objective To investigate the effect of microRNA-223 （miR-223） on prostate cancer cell damage by regulating the Kelch?like epichlorohydrin related protein 1 （Keap1）/nuclear factor E2 related factor 2 （Nrf2）/antioxidant response element （ARE） signaling pathway. Methods The prostate cancer cell line PC3 was cultured and randomly divided into control， down-regulated miR-223， and up-regulated miR-223 groups. Changes in miR-223 expression， cell proliferation rate， cell migration number， cell invasion number， apoptosis rate， and expression level of Keap1/Nrf2/ARE signaling pathway were explored. Results Compared with the control group， the cell invasion number， cell migration number， cell proliferation rate， Nrf2 and ARE expression increased at 24， 48 and 72 h in down-regulated miR-223 group， while the expressions of miR-223， Keap1 and apoptosis rate decreased （P < 0.05）. Compared with the down-regulated miR-223 group， 24， 48 and 72 h cell proliferation rate， cell invasion number， cell migration number， ARE and Nrf2 expression decreased in the up-regulated miR-223 group， while miR-223， apoptosis rate and Keap1 expression increased （P < 0.05）. Conclusion Regulation of miR-223 effectively ameliorates prostate cell injury， and the mechanism may be related to the Keap1/Nrf2/ARE signaling pathway.
Zhishi WANG , Guiling LI , Jingguo CHEN , Hong. WANG . Effects of miR⁃223 on prostate cancer cell damage by regulating Keap1/Nrf2/ARE signaling pathway[J]. The Journal of Practical Medicine, 2024 , 40(17) : 2375 -2380 . DOI: 10.3969/j.issn.1006-5725.2024.17.004
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