The Journal of Practical Medicine >
GATA3 mediates the effect of miR⁃21/PTEN axis on the proliferation and invasion of endometrial cancer cells
Received date: 2023-06-16
Online published: 2024-07-30
Objective To analyze the effects of GATA binding protein 3 （GATA3） mediated mini RNA-21 （miR-21）/phosphatase and tensin homologue （PTEN） axis missing from human chromosome Chromosome 10 on the proliferation and invasion of endometrial cancer cells. Methods HEC-1-A cells were transfected and divided into control group， GATA3 empty plasmid group， GATA3 overexpression plasmid group， GATA3 siRNA negative control group， and GATA3 siRNA group. Detect the expression levels of GATA3， miR-21， PTEN， proliferation， apoptosis rate， migration， and invasion in each group of cells. Results Compared with the hEEC group， the expression levels of GATA3 and miR-21 in cells of the HEC-1-A group， HEC-1-B group， and Ishikawa group increased， while the expression levels of PTEN decreased （P < 0.05）. Compared with the GATA3 empty plasmid group， the GATA3 overexpression plasmid group showed an increase in GATA3， miR-21 mRNA expression， proliferation rate， migration distance， number of invading cells， and Vimentin levels， while the PTEN mRNA expression， apoptosis rate， Caspase-9， Bax， and E-cadherin levels decreased （P < 0.05）； Compared with the GATA3 siRNA negative control group， the GATA3， miR-21 mRNA expression， proliferation rate， migration distance， number of invading cells， and Vimentin level decreased， while the PTEN mRNA expression， apoptosis rate， Caspase-9， Bax， and E-cadherin levels increased （P < 0.05）. Conclusion Downregulation of GATA3 expression can regulate the miR-21/PTEN axis， slow down the proliferation of HEC-1-A cells， and promote apoptosis of HEC-1-A cells.
Fahui WANG , Qingchun DENG , Jiajia LIN , Chunfei. CHEN . GATA3 mediates the effect of miR⁃21/PTEN axis on the proliferation and invasion of endometrial cancer cells[J]. The Journal of Practical Medicine, 2024 , 40(15) : 2069 -2074 . DOI: 10.3969/j.issn.1006-5725.2024.15.005
| 1 | 王赛凤，史蔚，谢咸晶. RNA结合基序5基因敲除子宫内膜癌细胞系及其作用研究［J］. 实用医学杂志， 2022，38（10）：1203-1207. doi:10.3969/j.issn.1006-5725.2022.10.006 |
| 2 | ARCIUOLO D， TRAVAGLINO A， RAFFONE A， et al. TCGA Molecular Prognostic Groups of Endometrial Carcinoma： Current Knowledge and Future Perspectives［J］. Int J Mol Sci， 2022，23（19）：11-16. doi:10.3390/ijms231911684 |
| 3 | YEN T T， WANG T L， FADER A N， et al. Molecular Classification and Emerging Targeted Therapy in Endometrial Cancer［J］. Int J Gynecol Pathol， 2020，39（1）：26-35. doi:10.1097/pgp.0000000000000585 |
| 4 | ZHAO S， LI W， YU W， et al. Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys［J］. Theranostics， 2021，11（18）：8660-8673. doi:10.7150/thno.62820 |
| 5 | TURNHAM D J， BULLOCK N， DASS M S， et al. The PTEN Conundrum： How to Target PTEN-Deficient Prostate Cancer［J］. Cells， 2020，9（11）：23-26. doi:10.3390/cells9112342 |
| 6 | YANG H， ZHANG H， LUAN Y， et al. Noncoding genetic variation in GATA3 increases acute lymphoblastic leukemia risk through local and global changes in chromatin conformation［J］. Nat Genet， 2022，54（2）：170-179. doi:10.1038/s41588-021-00993-x |
| 7 | YEN T T， WANG T L， FADER A N， et al. Molecular Classification and Emerging Targeted Therapy in Endometrial Cancer［J］. Int J Gynecol Pathol， 2020，39（1）：26-35. doi:10.1097/pgp.0000000000000585 |
| 8 | VAN DEN HEERIK A S V M， HOREWEG N， DE BOER S M， et al. Adjuvant therapy for endometrial cancer in the era of molecular classification： radiotherapy， chemoradiation and novel targets for therapy［J］. Int J Gynecol Cancer， 2021，31（4）：594-604. doi:10.1136/ijgc-2020-001822 |
| 9 | REN X， LIANG J， ZHANG Y， et al. Single-cell transcriptomic analysis highlights origin and pathological process of human endometrioid endometrial carcinoma［J］. Nat Commun， 2022，13（1）：6300-6308. doi:10.1038/s41467-022-33982-7 |
| 10 | PASSARELLO K， KURIAN S， VILLANUEVA V. ENDOMETRIAL CANCER： An Overview of Pathophysiology， Management， and Care［J］. Semin Oncol Nurs， 2019，35（2）：157-165. doi:10.1016/j.soncn.2019.02.002 |
| 11 | REN G， LAI B， HARLY C， et al. Transcription factors TCF-1 and GATA3 are key factors for the epigenetic priming of early innate lymphoid progenitors toward distinct cell fates［J］. Immunity， 2022，55（8）：1402-1413. doi:10.1016/j.immuni.2022.06.019 |
| 12 | ZHU Z， SHEN H， XU J， et al. GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells［J］. Drug Resist Updat， 2023，69（5）：10-14. doi:10.1016/j.drup.2023.100974 |
| 13 | ZHANG M， WANG N， SONG P， et al. LncRNA GATA3-AS1 facilitates tumour progression and immune escape in triple-negative breast cancer through destabilization of GATA3 but stabilization of PD-L1［J］. Cell Prolif， 2020，53（9）：12-15. doi:10.1111/cpr.12855 |
| 14 | EL-ARABEY A A， ABDALLA M. GATA3 as an immunomodulator in obesity-related metabolic dysfunction associated with fatty liver disease， insulin resistance， and type 2 diabetes［J］. Chem Biol Interact， 2022，366（7）：11-14. doi:10.1016/j.cbi.2022.110141 |
| 15 | QU Y Y， ZHAO R， ZHANG H L， et al. Inactivation of the AMPK-GATA3-ECHS1 Pathway Induces Fatty Acid Synthesis That Promotes Clear Cell Renal Cell Carcinoma Growth［J］. Cancer Res， 2020，80（2）：319-333. doi:10.1158/0008-5472.can-19-1023 |
| 16 | FERRER A I， EINSTEIN E， MORELLI S S. Bone Marrow-Derived Cells in Endometrial Cancer Pathogenesis： Insights from Breast Cancer［J］. Cells， 2022，11（4）：714. doi:10.3390/cells11040714 |
| 17 | GE X， LI M， YIN J， et al. Fumarate inhibits PTEN to promote tumorigenesis and therapeutic resistance of type2 papillary renal cell carcinoma［J］. Mol Cell， 2022，82（7）：1249-1260. doi:10.1016/j.molcel.2022.01.029 |
| 18 | KMIOLEK T， PARADOWSKA-GORYCKA A. miRNAs as Biomarkers and Possible Therapeutic Strategies in Rheumatoid Arthritis［J］. Cells， 2022，11（3）：452. doi:10.3390/cells11030452 |
| 19 | 袁峰，张书力，李少军，等. 血清miR-21、缺氧诱导因子-1α水平在带状疱疹患者治疗前后变化及评估后遗神经痛的分析［J］. 实用医学杂志， 2023，39（3）：364-368. doi:10.3969/j.issn.1006-5725.2023.03.018 |
| 20 | HASHEMI M， MIRDAMADI M S A， TALEBI Y， et al. Pre-clinical and clinical importance of miR-21 in human cancers： Tumorigenesis， therapy response， delivery approaches and targeting agents［J］. Pharmacol Res， 2023，187（7）：10-18. doi:10.1016/j.phrs.2022.106568 |
| 21 | BIBO-VERDUGO B， SALVESEN G S. Caspase mechanisms in the regulation of inflammation［J］ Mol Aspects Med， 2022，88（7）：10-15. doi:10.1016/j.mam.2022.101085 |
| 22 | CAO K， RILEY J S， HEILIG R， et al. Mitochondrial dynamics regulate genome stability via control of caspase-dependent DNA damage［J］. Dev Cell， 2022，57（10）：1211-1225. doi:10.1016/j.devcel.2022.03.019 |
| 23 | KIELBIK M， SZULC-KIELBIK I， KLINK M. E-Cadherin Expression in Relation to Clinicopathological Parameters and Survival of Patients with Epithelial Ovarian Cancer［J］. Int J Mol Sci， 2022，23（22）：14383. doi:10.3390/ijms232214383 |
| 24 | YAN G， WAN Z， ZHONG Y， et al. LncRNA MCM3AP-AS1 Regulates miR-21/PTEN Axis to Affect Cervical Squamous Cell Carcinoma Cell Proliferation and Apoptosis［J］. Crit Rev Eukaryot Gene Expr， 2022，32（4）：49-56. doi:10.1615/critreveukaryotgeneexpr.2022041014 |
| 25 | TAN C， DU H， WANG Y， et al. LncRNA GABPB1-IT1 inhibits the tumorigenesis of renal cancer via the miR-21/PTEN axis. J Biochem Mol Toxicol， 2023，37（4）：23-28. doi:10.1002/jbt.23288 |
/
| 〈 |
|
〉 |