The Journal of Practical Medicine >
The effect of miR⁃143⁃3p on pyroptosis of ulcerative colitis cells by regulating TLR2/NF⁃κB/NLRP3
Received date: 2023-11-25
Online published: 2024-07-30
Objective To study the effect of miR-143-3p on pyroptosis of HCT-116 cells induced by LPS + ATP. Methods The targeting relationship between miR-143-3 p and TLR2 gene was detected by dual luciferase. The pyroptosis model was established after transfection of miR-143-3p mimic. Cell apoptosis was detected by flow cytometry， and the activity of Caspase-1 and LDH was detected by biochemical method. The levels of IL-1β and IL-18 were detected by ELISA. The mRNA levels of NF-κB， TLR2， NLRP3 and GSDMD were detected by q-PCR. The expression of NLRP3 and ASC was detected by immunofluorescence. The protein expressions of TLR2， GSDMD， p-NF-κB p65 and cleave Caspase-1 were detected by Western blot. Results Dual luciferase assay showed that miR-143-3p targeted TLR2 expression. The expressions of NF-κB， TLR2， NLRP3， GSDMD mRNA and TLR2， GSDMD， p-NF-κB p65， cleave Caspase-1， ASC， NLRP3 protein in miR-143-3p mimic group and si TLR2 group were lower than those in model group. The activity of Caspase-1 and the content of LDH， IL-1β and IL-18 decreased. Conclusion miR-143-3p regulates pyroptosis of ulcerative colitis cells by targeting TLR2 gene and regulating NF-κB/NLRP3/Caspase-1 signaling pathway.
Key words： ulcerative colitis; cell pyroptosis; miR-143-3p; TLR2; TLR2/ NF-κB/NLRP3
Xiuli SHI , Jiaqi CHEN , Fan ZHU , Juan ZENG , Na. WU . The effect of miR⁃143⁃3p on pyroptosis of ulcerative colitis cells by regulating TLR2/NF⁃κB/NLRP3[J]. The Journal of Practical Medicine, 2024 , 40(15) : 2056 -2062 . DOI: 10.3969/j.issn.1006-5725.2024.15.003
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