The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 11: 1519 - 1525

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.11.009

Basic Research

TMSB10 promotes gastric cancer proliferation and glycolysis based on activation of AMPK/mTOR signaling pathway

  • Wei WANG ,
  • Xinxin ZHANG ,
  • Guanghui WANG ,
  • Jie ZHANG ,
  • Anran CHEN ,
  • Jianguang. JIA
Expand
  • *.Department of Oncological Surgery，Second Affiliated Hospital，Bengbu Medical College，Bengbu 233040，China

Received date: 2023-08-09

  Online published: 2024-06-13

Fold

Abstract

Objective To investigate the expression of Thymosinβ10 （TMSB10） in gastric cancer and its molecular mechanism in promoting the progression of gastric cancer. Methods We collected pathological sections of 70 patients with gastric cancer in our hospital， detected expression of TMSB10 in gastric adenocarcinoma by immunohistochemistry， and analyzed its prognostic effect. In order to study the mechanism of action， the effects of TMSB10 on the proliferation and glycolysis of gastric cancer cells and its related mechanism were observed by transfection technique， CCK8 test， EDU assay， Transwell assay， scratch test， glycolysis assay and Western blot. Results Immunohistochemistry showed that TMSB10was highly expressed in gastric cancer， while the expression level of TMSB10 was correlated with tumor size （P = 0.032）， TNM stage （P = 0.002） and distant metastasis. In the mechanism study， we found that overexpression of TMSB10 promoted the proliferation， invasion， migration and glycolytic phenotype of gastric cancer cells through in vitro CCK-8 test， EDU assay， Transwel assay and glycolysis assay， and vice versa. Western blot results showed that overexpression of TMSB10 may regulate the occurrence of gastric cancer through up-regulation of the AMPK/mTOR signaling pathway. Conclusions TMSB10 promotes the proliferation， invasion， migration and glycolysis gastric cancer through the AMPK/mTOR signaling pathway.

Key words： TMSB10; glycolysis; gastric cancer; AMPK/mTOR signaling pathway

Cite this article

Wei WANG , Xinxin ZHANG , Guanghui WANG , Jie ZHANG , Anran CHEN , Jianguang. JIA . TMSB10 promotes gastric cancer proliferation and glycolysis based on activation of AMPK/mTOR signaling pathway[J]. The Journal of Practical Medicine, 2024 , 40(11) : 1519 -1525 . DOI: 10.3969/j.issn.1006-5725.2024.11.009

References

1 SIEGEL R L， MILLER K D， WAGLEE N S， et al. Cancer statistics， 2023［J］. CA Cancer J Clin， 2023，73（1）：17-48. doi:10.3322/caac.21763
2 SUNG H， FERLAY J， SIEGEL R L， et al. Global Cancer Statistics 2020： GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries［J］. CA Cancer J Clin， 2021，71（3）：209-249. doi:10.3322/caac.21660
3 BALAKRISHNAN M， GEORGE R， SHARMA A， et al. Changing Trends in Stomach Cancer Throughout the World［J］. Curr Gastroenterol Rep， 2017，19（8）：36-40. doi:10.1007/s11894-017-0575-8
4 YAN Z， YAN Q， SONG Y， et al. TMSB10， a potential prognosis prediction biomarker， promotes the invasion and angiogenesis of gastric cancer［J］. J Gastroenterol Hepatol， 2021，36（11）：3102-3112. doi:10.1111/jgh.15576
5 SONG C， SU Z， GUO J. Thymosin β 10 is overexpressed and associated with unfavorable prognosis in hepatocellular carcinoma［J］. Biosci Rep， 2019，39（3）：BSR20182355. doi:10.1042/bsr20182355
6 张萌， 刘旭明， 麦皓尘，等. SLC7A11在KRAS突变胰腺癌中的表达及临床意义［J］. 实用医学杂志， 2022，38（24）：3125-3128. doi:10.3969/j.issn.1006?5725.2022.24.018
7 ZHOU Y， CHEN Y， SHI Y， et al. FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway［J］. J Clin Invest， 2023，133（3）：1-8. doi:10.1172/jci158705
8 LEE M， CHO H J， PARK K S， et al. ELK3 Controls Gastric Cancer Cell Migration and Invasion by Regulating ECM Remodeling-Related Genes［J］. Int J Mol Sci， 2022，23（7）：1-9. doi:10.3390/ijms23073709
9 CHEN Z， YUAN L， LI X， et al. BMP2 inhibits cell proliferation by downregulating EZH2 in gastric cancer［J］. Cell Cycle， 2022，21（21）：2298-2308. doi:10.1080/15384101.2022.2092819
10 LI S， GUO D， SUN Q， et al. MAPK4 silencing in gastric cancer drives liver metastasis by positive feedback between cancer cells and macrophages［J］. Exp Mol Med， 2023，55（2）：457-469. doi:10.1038/s12276-023-00946-w
11 GUO R， CUI X， LI X， et al. CircMAN1A2 is upregulated by Helicobacter pylori and promotes development of gastric cancer［J］. Cell Death Dis， 2022，13（4）：409-415. doi:10.1038/s41419-022-04811-y
12 LI Y， LI L， LIU H， et al. CPNE1 silencing inhibits cell proliferation and accelerates apoptosis in human gastric cancer［J］. Eur J Pharm Sci， 2022，177（1）：106278-106289. doi:10.1016/j.ejps.2022.106278
13 GENG S， PENG W， WANG X， et al. ARIH2 regulates the proliferation， DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination［J］. Cell Death Dis， 2022，13（6）：564-578. doi:10.1038/s41419-022-04965-9
14 ZHANG G， ZHU Q， YAN X， et al. HECTD3 promotes gastric cancer progression by mediating the polyubiquitination of c-MYC［J］. Cell Death Discov， 2022，8（1）：185-195. doi:10.1038/s41420-022-01001-9
15 LI D， WANG Y， DONG C， et al. CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1［J］. Oncogene， 2023，42（2）：83-98. doi:10.1038/s41388-022-02537-x
16 YAN C， YANG H， SU P， et al. OTUB1 suppresses Hippo signaling via modulating YAP protein in gastric cancer［J］. Oncogene， 2022，41（48）：5186-5198. doi:10.1038/s41388-022-02507-3
17 LI J， ZHOU S， LI H， et al. PTEN/AKT upregulation of TMSB10 contributes to lung cancer cell growth and predicts poor survival of the patients［J］. Biosci Biotechnol Biochem， 2021，85（4）：805-813. doi:10.1093/bbb/zbaa113
18 WANG C， HE Y， YOU Z， et al. TMSB10 Promotes Progression of Clear Cell Renal Cell Carcinoma via JUN Transcription Regulation［J］. Ann Clin Lab Sci， 2022，52（2）：230-239.
19 PAN Q， CHENG G， LIU Y， et al. TMSB10 acts as a biomarker and promotes progression of clear cell renal cell carcinoma［J］. Int J Oncol， 2020，56（5）：1101-1114.
20 XIONG Y， QI Y， PAN Z， et al. Pancancer landscape analysis of the thymosin family identified TMSB10 as a potential prognostic biomarker and immunotherapy target in glioma［J］. Cancer Cell Int， 2022，22（1）：294-305. doi:10.1186/s12935-022-02698-5
21 LEE S H， ZHANG W， CHOI J J， et al. Overexpression of the thymosin beta-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis［J］. Oncogene， 2001，20（46）：6700-6706. doi:10.1038/sj.onc.1204683
22 SRIBENJA S， SAWANYAWISUTH K， KRAIKLANG R， et al. Suppression of thymosin β10 increases cell migration and metastasis of cholangiocarcinoma［J］. BMC Cancer， 2013，13（1）：430-438. doi:10.1186/1471-2407-13-430
23 FANG G， ZHANG P， LIU J， et al. Inhibition of GSK-3β activity suppresses HCC malignant phenotype by inhibiting glycolysis via activating AMPK/mTOR signaling［J］. Cancer Lett， 2019，463（1）：11-26. doi:10.1016/j.canlet.2019.08.003
Options
Outlines

/

〈 〉