The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 11: 1513 - 1518

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.11.008

Basic Research

Effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα in blood CD4+ Th1 cells of patients with allergic rhinitis

  • Junling WANG ,
  • Mengmeng ZHAN ,
  • Fangqiu GU ,
  • Yifei LI ,
  • Zhaolong ZHANG ,
  • Congyi ZHAO ,
  • Danyang ZHAO ,
  • Hui ZHENG ,
  • Yijie ZHANG ,
  • Bingyu. QIN
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  • *.Department of Critical Care Medicine，Henan Provincial People's Hospital，Zhengzhou University People's Hospital，Zhengzhou 450003，China
    *.Department of Respiration，Huaihe Hospital of Henan University，Kaifeng 475000，China
    *.Allergy and Clinical Immunology Research Centre，the First Affiliated Hospital of Jinzhou Medical University，Jinzhou 121001，China

Received date: 2023-09-28

  Online published: 2024-06-13

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Abstract

Objective To investigate the effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα protein in peripheral blood CD4+ Th1 cells of healthy control subjects （HC） and patients with allergic rhinitis （AR）， and on the expressions of IL-18， IL-18BPa and IL-18Rα mRNA in the peripheral blood CD4+ T cells. Methods Blood samples were collected from patients with rhinitis for negative skin prick test （AR-）， rhinitis for positive skin prick test （AR+） and HC. Flow cytometry was used to evaluate the effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα protein in CD4+ Th1 cells. The expressions of IL-18， IL-18BPa and IL-18Rα mRNA in CD4+ T cells were determined by qPCR. Results Compared with HC， increased IL-18 while decreased IL-18BPa expressions in Th1 cells of AR- and AR+ patients were observed， increased IL-18Rα expression in Th1 cells of AR+ patients was also found. Additionally， allergens induced elevated expression of IL-18Rα protein in Th1 cells of HC， and induced elevated mRNA expressions of IL-18， IL-18BPa and IL-18Rα in isolated blood CD4+ T cells of AR+ patients and HC. Conclusion Allergens may be involved in the pathogenesis of AR by inducing the expressions of IL-18 and IL-18Rα in blood CD4+ Th1 cells.

Key words： allergic rhinitis; CD4+ Th1 cell; IL-18; IL-18Rα

Cite this article

Junling WANG , Mengmeng ZHAN , Fangqiu GU , Yifei LI , Zhaolong ZHANG , Congyi ZHAO , Danyang ZHAO , Hui ZHENG , Yijie ZHANG , Bingyu. QIN . Effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα in blood CD4+ Th1 cells of patients with allergic rhinitis[J]. The Journal of Practical Medicine, 2024 , 40(11) : 1513 -1518 . DOI: 10.3969/j.issn.1006-5725.2024.11.008

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