过敏原对过敏性鼻炎患者外周血CD4+ Th1IL-18、IL-18BPa和IL-18Rα表达的影响

doi:10.3969/j.issn.1006-5725.2024.11.008

Abstract

Abstract:

Objective To investigate the effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα protein in peripheral blood CD4⁺ Th1 cells of healthy control subjects （HC） and patients with allergic rhinitis （AR）， and on the expressions of IL-18， IL-18BPa and IL-18Rα mRNA in the peripheral blood CD4⁺ T cells. Methods Blood samples were collected from patients with rhinitis for negative skin prick test （AR^-）， rhinitis for positive skin prick test （AR⁺） and HC. Flow cytometry was used to evaluate the effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα protein in CD4⁺ Th1 cells. The expressions of IL-18， IL-18BPa and IL-18Rα mRNA in CD4⁺ T cells were determined by qPCR. Results Compared with HC， increased IL-18 while decreased IL-18BPa expressions in Th1 cells of AR^- and AR⁺ patients were observed， increased IL-18Rα expression in Th1 cells of AR⁺ patients was also found. Additionally， allergens induced elevated expression of IL-18Rα protein in Th1 cells of HC， and induced elevated mRNA expressions of IL-18， IL-18BPa and IL-18Rα in isolated blood CD4⁺ T cells of AR⁺ patients and HC. Conclusion Allergens may be involved in the pathogenesis of AR by inducing the expressions of IL-18 and IL-18Rα in blood CD4⁺ Th1 cells.

Key words: allergic rhinitis, CD4⁺ Th1 cell, IL-18, IL-18Rα

CLC Number:

R392.8

Junling WANG,Mengmeng ZHAN,Fangqiu GU,Yifei LI,Zhaolong ZHANG,Congyi ZHAO,Danyang ZHAO,Hui ZHENG,Yijie ZHANG,Bingyu. QIN. Effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα in blood CD4⁺ Th1 cells of patients with allergic rhinitis[J]. The Journal of Practical Medicine, 2024, 40(11): 1513-1518.

Figures/Tables 4

References 23

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志愿者	病例	年龄（岁）	性别（例）		病史（年）	发病年龄（岁）
志愿者	病例	年龄（岁）	女	男	病史（年）	发病年龄（岁）
HC	23	28.4 ± 12.9	13	10	0	-
AR^-	21	34.2 ± 14.9	12	9	6.0 ± 4.7	28.3 ± 13.2
AR⁺	24	33.5 ± 12.5	14/10		5.4 ± 4.8	28.2 ± 12.9

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Effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα in blood CD4⁺ Th1 cells of patients with allergic rhinitis

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Effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα in blood CD4+ Th1 cells of patients with allergic rhinitis

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Effects of allergens on the expressions of IL-18， IL-18BPa and IL-18Rα in blood CD4⁺ Th1 cells of patients with allergic rhinitis