The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 10: 1382 - 1388

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.10.009

Clinical Research

Correlations of miR⁃203a⁃3p and PHOX2B expression in glioma tissues with clinical features and prognosis

  • Qi ZHANG ,
  • Jia HU ,
  • Hui LI ,
  • Jin WANG ,
  • Zhongde LI ,
  • Zhiyuan. HU
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  • *.Second Department of Neurosurgery，Zhangye People's Hospital，Hexi University，Zhangye 734000，China

Received date: 2023-11-07

  Online published: 2024-05-21

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Abstract

Objective To investigate the correlations microRNA-203a-3p （miR-203a-3p） and paired allographic box protein 2B （PHOX2B） expression levels in glioma tissues with clinical features and prognosis. Methods Ninety-six glioma patients admitted to our hospital from June 2017 to April 2020 were included in the study. The resected glioma tissues and corresponding paracancerous tissues from the patients were collected intraoperatively. qRT-PCR and immunohistochemistry were conducted to detect the expression of miR-203a-3p and PHOX2B mRNA in glioma tissues and paracancerous groups. Pearson's method was used to analyze the correlation between miR-203a-3p and PHOX2B mRNA. Kaplan-Meier survival curves were used to analyze the correlations of miR-203a-3p and PHOX2B mRNA with the prognosis of glioma patients. COX regression was used to analyze the risk factors affecting the prognosis of glioma patients. Results The miR-203a-3p level and positive expression rate of glioma tissues were significantly lower than that of paracancerous tissues （P < 0.05）， and the PHOX2B mRNA level and positive expression rate were significantly higher than that of paracancerous tissues （P < 0.05）. By Pearson correlation analysis， the miR-203a-3p and PHOX2B mRNA levels of glioma patients were negatively correlated （P < 0.05）. miR-203a-3p and PHOX2B were associated with KPS score， tumor tissue necrosis， and WHO grading （P < 0.05）. Kaplan-Meier curve analysis yielded the results that the survival rate of the miR-203a-3p highly expressed group was significantly higher than that of the lowly expressed group （P < 0.05）， and the survival rate of the PHOX2B mRNA highly expressed group was significantly lower than that of the lowly expressed group （P < 0.05）. COX regression analysis showed that miR-203a-3p， PHOX2B mRNA， tumor tissue necrosis， and WHO grading were the risk factors affecting the prognosis of glioma patients （P < 0.05）. Conclusion The expression level of miR-203a-3p is obviously reduced and the expression level of PHOX2B mRNA is obviously increased in glioma， both showing close correlation with clinical characteristics and prognosis.

Key words： micro RNA-203a-3p; paired-like homebox 2B; glioma; clinical characteristics; prognosis

Cite this article

Qi ZHANG , Jia HU , Hui LI , Jin WANG , Zhongde LI , Zhiyuan. HU . Correlations of miR⁃203a⁃3p and PHOX2B expression in glioma tissues with clinical features and prognosis[J]. The Journal of Practical Medicine, 2024 , 40(10) : 1382 -1388 . DOI: 10.3969/j.issn.1006-5725.2024.10.009

References

1 李琳，刘超，邵将，等. 肿瘤电场治疗在神经胶质瘤治疗中的研究进展［J］. 实用医学杂志， 2022， 38（22）： 2763-2767. doi:10.3969/j.issn.1006-5725.2022.22.001
2 YANG K， WU Z， ZHANG H， et al. Glioma targeted therapy： insight into future of molecular approaches［J］. Mol Cancer， 2022， 21（1）：39. doi:10.1186/s12943-022-01513-z
3 XU S， TANG L， LI X， et al. Immunotherapy for glioma： Current management and future application［J］. Cancer Lett， 2020， 476（1）： 1-12. doi:10.1016/j.canlet.2020.02.002
4 HENDERSON F， ABDULLAH K G， VERMA R， et al. Tractography and the connectome in neurosurgical treatment of gliomas： the premise， the progress， and the potential［J］. Neurosurg Focus， 2020， 48（2）： E6. doi:10.3171/2019.11.focus19785
5 XIN S， HUANG K， ZHU X G. Non-coding RNAs： Regulators of gliomacell epithelial mesenc- hymal transformation ［J］. Pathol Res Pract， 2019， 215（9）：152539-152548. doi:10.1016/j.prp.2019.152539
6 李天雄， 孙志鹏， 尹刚， 等. miR-133a-3p通过抑制氧化还原因子1抑制肝细胞恶性生物学行为［J］.中国免疫学杂志， 2020， 36（10）：1217-1223.
7 SYEDA Z ALI， LANGDEN S S S， MUNKHZUL C， et al. Regulatory Mechanism of MicroRNA Expression in Cancer［J］. Int J Mol Sci， 2020， 21（5）： 1723. doi:10.3390/ijms21051723
8 WANG Z， ZHAO Z， YANG Y， et al. MiR-99b-5p and miR-203a-3pFunction as Tumor Suppressors by Targeting IGF-IR in Gas-trie Cancer［J］.Sei Rep， 2018， 8（1）：10119-10131. doi:10.1038/s41598-018-27583-y
9 CARDANI S， DI LASCIO S， BELPERIO D， et al.Desogestrel down-rgulates PHOX2B and its target genes in pro-gesterone responsiveneuroblastoma cells ［J］.Exp Cell Res， 2018， 370（2） ： 671-679. doi:10.1016/j.yexcr.2018.07.032
10 刘江华，臧博伦，刘炜，等. 高危神经母细胞瘤患儿骨髓中PHOX2B及GD2的表达及临床意义［J］. 中国现代医药杂志， 2022， 24（6）：1-5. doi:10.3969/j.issn.1672-9463.2022.06.001
11 苏昌亮，李丽，陈小伟，等. 2016年WHO中枢神经系统肿瘤分类总结［J］. 放射学实践， 2016， 31（7）： 570-579.
12 中国脑胶质瘤协作组，中国脑胶质瘤基因组图谱计划. 中国脑胶质瘤分子诊疗指南［J］. 中华神经外科杂志， 2014， 30（5）： 435-444. doi:10.3760/cma.j.issn.1001-2346.2014.05.002
13 GUO X， SUI R， PIAO H. Exosomes-mediated crosstalk between glioma and immune cells in the tumor microenvironment［J］. CNS Neurosci Ther， 2023， 29（8）： 2074-2085. doi:10.1111/cns.14239
14 RUFF M， KIZILBASH S， BUCKNER J. Further understanding of glioma mechanisms of pathogenesis： implications for therapeutic development［J］. Expert Rev Anticancer Ther， 2020， 20（5）：355-363. doi:10.1080/14737140.2020.1757440
15 URIBE-CARDENAS R， GIANTINI-LARSEN A M， GARTON A， et al. Innovations in the Diagnosis and Surgical Management of Low-Grade Gliomas［J］. World Neurosurg， 2022， 166（1）： 321-327. doi:10.1016/j.wneu.2022.06.070
16 SEYEDMIRZAEI H， SHOBEIRI P， TURGUT M， et al. VEGF levels in patients with glioma： a systematic review and meta-analysis［J］. Rev Neurosci， 2020， 32（2）： 191-202. doi:10.1515/revneuro-2020-0062
17 GAO Q， ZHOU H， WANG G， et al. A Model for Predicting Clinical Prognosis in Patients with WHO Grade 2 Glioma［J］. J Oncol， 2022， 2022（1）： 2795939. doi:10.1155/2022/2795939
18 ZHANG J， REN C， HAO S J， et al. MiRNA-491-5p inhibits cell proliferation， invasion and migration via targeting JMJD2B and serves as a potential biomarker in gastric cancer［J］. Am J Transl Res， 2018， 10（2）： 525-534.
19 MENON A， ABD-AZIZ N， KHALID K， et al. miRNA： A Promising Therapeutic Target in Cancer［J］. Int J Mol Sci， 2022， 23（19）： 11502. doi:10.3390/ijms231911502
20 LIANG J， SUN T， WANG G， et al. Clinical significance and functions of miR-203a-3p/AVL9 axis in human non-small-cell lung cancer［J］. Per Med， 2020， 17（4）： 271-282. doi:10.2217/pme-2019-0108
21 杨雯迪，李亚军. miR-203a-3p靶向调控VEGF-C表达对卵巢癌恶性生物学特征的影响［J］. 中国妇幼健康研究， 2022， 33（1）： 66-72. doi:10.3969/j.issn.1673-5293.2022.01.012
22 AN N， ZHENG B. MiR-203a-3p Inhibits Pancreatic Cancer Cell Proliferation， EMT， and Apoptosis by Regulating SLUG［J］. Technol Cancer Res Treat， 2020， 19（1）：1533033819898729. doi:10.1177/1533033819898729
23 ALEXANDRESCU S， PAULSON V， DUBUC A， et al. PHOX2B is a reliableimmunomarker in distinguishing peripheral neuroblastie tumourfrom CNS embryonal tumours ［J］. Histopathology， 2018， 73 （3）：483-491，. doi:10.1111/his.13648
24 李静羽，高吉照. 同源异型盒蛋白2B在外周神经母细胞性肿瘤中表达及临床意义［J］. 临床军医杂志， 2021， 49（1）： 89-91.
25 FAN Y， CHEN P， RAZA M U， et al. Alteredexpression of phox2transcription factors in the locus coeruleus in major depressivedisorder mimicked by chronic stress and corticosterone treatment invivo and in vitro［J］. Neuroscience， 2018， 393（1）： 123-137. doi:10.1016/j.neuroscience.2018.09.038
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