The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 3: 365 - 370

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.03.015

Clinical Research

Expression of CENPF and miR⁃1⁃3p in the serum of patients with advanced gastric cancer and their correlation with prognosis

  • Jian ZHAO ,
  • Songjie LIU ,
  • Guanchao ZHANG ,
  • Yuhou SHEN ,
  • Fengchen LI ,
  • Bing XU
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  • General Surgery Department of the Fourth Clinical College of Xinxiang Central Hospital/Xinxiang Medical College，Xinxiang 453000，China

Received date: 2023-08-27

  Online published: 2024-02-22

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Abstract

Objective To investigate the expression of centromeric protein F （CENPF） and microribonucleic acid 1?3p （miR?1?3p） in the serum of patients with advanced gastric cancer and their correlation with prognosis. Methods Sixty patients with advanced gastric cancer admitted to our hospital from March 2019 to March 2020 were collected as the study group， while 60 healthy volunteers who underwent physical examinations at our hospital's physical examination center during the same period were collected as the control group. Real?time fluorescence quantitative PCR （qRT?PCR） method was applied to detect the expression levels of serum CENPF and miR?1?3p in each group； Pearson method was applied to analyze the correlation between serum levels of CENPF and miR?1?3p； Kaplan?Meier method was applied to analyze the relationship between the expression of CENPF， miR?1?3p， and prognosis in patients with advanced gastric cancer； and COX regression was applied to analyze risk factors affecting the prognosis of patients with advanced gastric cancer. Results Compared with the control group， the CENPF level in the study group was obviously increased， while the miR?1?3p level was obviously reduced （P < 0.05）. The correlation analysis results showed that there was a negative correlation between serum CENPF and miR?1?3p levels in patients with advanced gastric cancer （r = -0.650，P < 0.001）. There were obvious differences in CENPF and miR?1?3p levels among different TNM stages and lymph node metastasis status （P < 0.05）. The 3?year survival rate of patients in the high expression group of CENPF was 19/30 （63.33%）， which was obviously lower than that in the low expression group， 28/30 （93.33%） （χ2 = 7.954， P < 0.001）； the 3?year survival rate of patients in high expression group of miR?1?3p was 29/30 （96.67%）， which was obviously higher than that in the low expression group， 18/30 （60.00%） （χ2 = 11.882， P = 0.001）. Multivariate COX regression analysis showed that TNM staging， lymph node metastasis， CENPF， and miR?1?3p expression were risk factors affecting the prognosis of patients with advanced gastric cancer（P < 0.05）. Conclusion The serum CENPF level in patients with advanced gastric cancer obviously increase， while miR?1?3p level obviously decrease， both of which are related to prognosis.

Key words： Centromeric protein F; Micro ribonucleic acid?1?3p; Middle to late stage gastric cancer; Prognosis

Cite this article

Jian ZHAO , Songjie LIU , Guanchao ZHANG , Yuhou SHEN , Fengchen LI , Bing XU . Expression of CENPF and miR⁃1⁃3p in the serum of patients with advanced gastric cancer and their correlation with prognosis[J]. The Journal of Practical Medicine, 2024 , 40(3) : 365 -370 . DOI: 10.3969/j.issn.1006-5725.2024.03.015

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