The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 1: 65 - 71

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.01.012

Basic Research

Expression of allograft inflammatory factor⁃1 in the testicular model of diabetes mellitus rats

  • Dechao LI ,
  • Mingjin ZHANG ,
  • Yibi LAN ,
  • Chunlei MA ,
  • Weijin. FU
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  • *.Department of Urology，Hezhou Hospital of Traditional Chinese Medicine，Hezhou 542899，China

Received date: 2023-04-24

  Online published: 2024-01-24

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Abstract

Objective To investigate the expression of allograft inflammatory factor-1 （AIF-1） in the testicular model of diabetes mellitus（DM） rats as well as its significance. Methods The rat model of DM testis （DMT） was established， which were randomly divided into the DM testis 4-week group （DMT4W）， DM testis s 8-week group （DMT8W） and the DM testis 12-week group （DMT12W）. The normal control group （NC group） was randomly divided into three subgroups： NC 4-week （NC4W）， NC 8-week （NC8W） and NC 12-week （NC12W）. The morphologic changes of testis in the different groups was detected by histopathology. The expression of AIF-1 protein was detected by immunohistochemistry. The expression of AIF-1 and NF-κB p65 protein was observed by immunofluorescence. Results The histopathological results suggested that the numbers of spermatogenic cells， sertoli cells， interstitial cells and sperms in the DMT group were significantly decreased， as compared with the NC group. The immunohistochemistry results showed that the expression of AIF-1 protein was significantly increased in the DMT group， as compared with the NC group （P < 0.05）. The intensity of AIF-1 and NF-κB p65 in the DMT group was significantly increased by immunofluorescence， as compared with the NC group. Conclusion The over expression of AIF-1 protein in DMT tissue suggests that it may play an important role in the pathological process of DMT and may become a new therapeutic target and diagnostic marker in the future.

Key words： allograft inflammatory factor-1; diabetes mellitus testis; rat; model; significance

Cite this article

Dechao LI , Mingjin ZHANG , Yibi LAN , Chunlei MA , Weijin. FU . Expression of allograft inflammatory factor⁃1 in the testicular model of diabetes mellitus rats[J]. The Journal of Practical Medicine, 2024 , 40(1) : 65 -71 . DOI: 10.3969/j.issn.1006-5725.2024.01.012

References

1 TAN S Y， MEI WONG J L， SIM Y J， et al. Type 1 and 2 diabetes mellitus： A review on current treatment approach and gene therapy as potential intervention［J］. Diabetes Metab Syndr，2019，13（1）：364-372.
2 BANDAY M Z， SAMEER A S， NISSAR S.Pathophysiology of diabetes： An overview［J］. Avicenna J Med，2020，10（4）：174-188.
3 FU J， WEI C， ZHANG W， et al. Gene expression profiles of glomerular endothelial cells support their role in the glomerulopathy of diabetic mice［J］. Kidney Int， 2018，94（2）：326-345.
4 罗富强， 余电柏， 谢康麒， 等. 糖尿病足溃疡创面修复相关机制的研究进展［J］.实用医学杂志，2023， 39 （2）： 158-163.
5 HUGHES F M， HIRSHMAN N A， INOUYE B M， et al. NLRP3 Promotes Diabetic Bladder Dysfunction and Changes in Symptom-Specific Bladder Innervation［J］.Diabetes， 2019， 68（2）：430-440.
6 TVRDA E， KOVAC J， BENKO F，et al. Characterization of the structural， oxidative， and immunological features of testis tissue from Zucker diabetic fatty rats［J］. Open Life Sci， 2022，17（1）：1383-1397.
7 WANG J， BAO B， FENG J， et al. Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway［J］. Med Sci Monit， 2021，27：e928232.
8 CONDORELI R A， LA VIGNERA S， MONGIOI L M， et al. Diabetes mellitus and infertility： Different pathophysiological effects in type 1 and type 2 on sperm function［J］. Front Endocrinol （Lausanne）， 2018，9：268.
9 LEISEGANG K， SENGUPTA P， AGARWAL A， et al. Obesity and male infertility： Mechanisms and management［J］. Andrologia， 2021，53（1）：e13617.
10 SIKORA M， KOPEC B， PIOTROWSKA K， et al. Role of allograft inflammatory factor-1 in pathogenesis of diseases［J］. Immunol Lett， 2020，218：1-4.
11 KOTTAISAMY C P D， RAJ D S， PRASANTH KUMAR V， et al.Experimental animal models for diabetes and its related complications-a review［J］. Lab Anim Res， 2021，37（1）：23.
12 FU Y， WANG X， ZHANG L， et al. Allograft inflammatory factor-1 enhances inflammation and oxidative stress via the NF-κB pathway in diabetic kidney disease［J］. Biochem Biophys Res Commun， 2022，614：63-69.
13 HINDI E A， WILLIAMS C J， ZEEF L A H，et al. Experimental long-term diabetes mellitus alters the transcriptome and biomechanical properties of the rat urinary bladder［J］. Sci Rep， 2021，11（1）：15529.
14 YANG K， WANG Q. 50 week ultrasound imaging and ultrastructural abnormalities of bladder after sugar diuresis and diabetes mellitus in rats［J］. Int Urol Nephrol， 2021，53（10）：1995-2005.
15 ZHANG Q， SUN S， ZHU C， et al. Expression of Allograft Inflammatory Factor-1 （AIF-1） in Hepatocellular Carcinoma［J］. Med Sci Monit， 2018，24：6218-6228.
16 MADERKA M， DVORAK V， HAMBALEK J，et al. Serum concentrations of S100-A11 and AIF-1 are elevated in cervical cancer patients with lymph node involvement［J］.Ceska Gynekol，2021，86（1）：17-21.
17 TVRDA E， KOVAC J， BENKO F， et al. Characterization of the structural， oxidative， and immunological features of testis tissue from Zucker diabetic fatty rats［J］. Open Life Sci， 2022，17（1）：1383-1397.
18 WANG J， BAO B， FENG J， et al. Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway［J］. Med Sci Monit， 2021，27：e928232.
19 ZHOU Y， LI X， YUAN X， et al. Role of allograft inflammatory factor-1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells［J］. Cell Biol Int， 2019，43（5）：495-505.
20 PIOTROWSKA K， SLUCZANOWSKA G S， KURZAWSKI M， et al. Over-Expression of Allograft Inflammatory Factor-1 （AIF-1） in Patients with Rheumatoid Arthritis［J］. Biomolecules， 2020，10（7）：1064.
21 OMOLAOYE T S， DU P. The effect of streptozotocin induced diabetes on sperm function： a closer look at AGEs， RAGEs， MAPKs and activation of the apoptotic pathway［J］. Toxicol Res，2020，37（1）：35-46.
22 FADDLADDEEN K A J. The possible protective and therapeutic effects of ginger and cinnamon on the testis and coda epididymis of streptozotocin-induced-diabetic rats： Histological and biochemical studies［J］. Saudi J Biol Sci， 2022，29（12）：103452.
23 HAN Y， JIA R， ZHANG J， et al. Hypoxia Attenuates Colonic Innate Immune Response and Inhibits TLR4/NF-κB Signaling Pathway in Lipopolysaccharide-Induced Colonic Epithelial Injury Mice［J］. J Interferon Cytokine Res， 2023，43（1）：43-52.
24 SHARMA D， BHATTACHARYA P， KALIA K， et al. Diabetic nephropathy： New insights into established therapeutic paradigms and novel molecular targets［J］.Diabetes Res Clin Pract，2017，128：91-108.
25 REN J， LIN Y， TANG J， et al. Allograft Inflammatory Factor-1 Mediates Macrophage-Induced Impairment of Insulin Signaling in Adipocytes［J］. Cell Physiol Biochem， 2018，47（1）：403-413.
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