同种异体移植物炎性因子-1在糖尿病睾丸大鼠模型中的表达及意义

doi:10.3969/j.issn.1006-5725.2024.01.012

Abstract

Abstract:

Objective To investigate the expression of allograft inflammatory factor-1 （AIF-1） in the testicular model of diabetes mellitus（DM） rats as well as its significance. Methods The rat model of DM testis （DMT） was established， which were randomly divided into the DM testis 4-week group （DMT4W）， DM testis s 8-week group （DMT8W） and the DM testis 12-week group （DMT12W）. The normal control group （NC group） was randomly divided into three subgroups： NC 4-week （NC4W）， NC 8-week （NC8W） and NC 12-week （NC12W）. The morphologic changes of testis in the different groups was detected by histopathology. The expression of AIF-1 protein was detected by immunohistochemistry. The expression of AIF-1 and NF-κB p65 protein was observed by immunofluorescence. Results The histopathological results suggested that the numbers of spermatogenic cells， sertoli cells， interstitial cells and sperms in the DMT group were significantly decreased， as compared with the NC group. The immunohistochemistry results showed that the expression of AIF-1 protein was significantly increased in the DMT group， as compared with the NC group （P < 0.05）. The intensity of AIF-1 and NF-κB p65 in the DMT group was significantly increased by immunofluorescence， as compared with the NC group. Conclusion The over expression of AIF-1 protein in DMT tissue suggests that it may play an important role in the pathological process of DMT and may become a new therapeutic target and diagnostic marker in the future.

Key words: allograft inflammatory factor-1, diabetes mellitus testis, rat, model, significance

CLC Number:

R695.5

Dechao LI,Mingjin ZHANG,Yibi LAN,Chunlei MA,Weijin. FU. Expression of allograft inflammatory factor⁃1 in the testicular model of diabetes mellitus rats[J]. The Journal of Practical Medicine, 2024, 40(1): 65-71.

Figures/Tables 7

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References 25

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组别	数量	体质量（g）	血糖（mmol/L）
NC4W	6	171.43 ± 12.36	7.70 ± 1.32
DMT4W	6	178.55 ± 15.29^*	7.47 ± 1.29^*
NC8W	6	179.23 ± 12.89	6.30 ± 0.77
DMT8W	6	182.80 ± 13.52^*	7.20 ± 1.17^*
NC12W	6	172.61 ± 10.34	6.97 ± 0.70
DMT12W	6	173.27 ± 6.29^*	7.35 ± 0.38^*

组别	数量	体质量（g）	血糖（mmol/l）	睾丸（g）
NC4W	6	289.55 ± 32.16	7.23 ± 0.94	1.67 ± 0.11
DMT4W	6	201.47 ± 28.12^**	28.30 ± 3.22^**	1.64 ± 0.10
NC8W	6	384.85 ± 17.84	7.23 ± 0.86	1.87 ± 0.10
DMT8W	5	203.44 ± 23.11^**	26.92 ± 1.98^**	1.65 ± 0.11^*
NC12W	6	469.93 ± 29.37	8.21 ± 1.95	1.94 ± 0.16
DMT12W	5	231.52 ± 36.86^**	26.20 ± 3.72^**	1.52 ± 0.03^*

组别	数量	低表达	高表达	P值	χ²值
4W				0.02	5.33
NC4W	6	5	1
DMT4W	6	1	5
8W				0.04	4.41
NC8W	6	5	1
DMT8W	5	2	3
12W				0.04	4.41
NC12W	6	5	1
DMT12W	5	2	3

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Expression of allograft inflammatory factor⁃1 in the testicular model of diabetes mellitus rats

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